Dystrophic mutation (dy2J) affecting regulation of lactate dehydrogenase (LDH) and pyruvate kinase (PK) in C57BL/6J mice

Summary The genotype difference (dystrophic vs nondystrophic) in the LDH isozymes is observed in kidney. These differences are evident only at birth and at early developmental stages (before the expression of dystrophic symptoms). The tissue specific genotype differences for PK are limited to the thigh muscle (M form) and heart (L form), after the onset of the condition. These differences may reflect the pleiotropic effect of the dy^2J locus during the temporal regulation of these and other enzymes implicated in muscular dystrophy (MD).This research was financed by a Natural Sciences and Engineering Research Council Canada grant to S.M.S.     

The antennae and mating behaviour ofDrosophila females

Summary The females ofDrosophila bipectinata andD. malerkotliana are able to discriminate between their own and alien males in the absence of antennae. Thus mate recognition seems to depend on contact chemoreceptors in these 2 species.We are grateful to the Head of the Department of Zoology, B.H.U. for providing the necessary facilities. Thanks are due to the U.G.C., New Delhi, India for awarding a junior research fellowship to O.P.S.     

Development of an enzyme linked immunosorbent assay (ELISA) for measurement of vitellogenin concentrations in cockroaches

Summary A competitive enzyme linked immunosorbent assay was developed for the quantification of a large lipoprotein, namely the yolk protein vitellogenin, in the haemolymph of cockroaches (Nauphoeta cinerea). This assay was found to be specific, reproducible and it has a high sensitivity (approximately 10 ng).Acknowledgments. We would like to express our thanks to Prof. H. Fey and Ms H. Pfister, Veterinary Bacteriological Institute, University of Berne, for introducing us into the ELISA methodology, to P. Beyeler for technical assistance and to M. Kaltenrieder for drawing the graphs. We also thank the Sandoz Foundation for a fellowship to M. Dumas and the Swiss National Science Foundation (grant No. 3. 188-0.77) for financial support.     

Receptors for thymosin fraction V on rat thymic lymphocytes

Summary Binding by rat thymus lymphocytes of thymosin V, labeled with colloidal gold, was studied. Under the experimental conditions employed at least 2.8% cells exhibited thymosin binding sites.This article originally appeared in Experientia 38 (5) 1982, p. 618 but was accompanied by imporperfigures. Supported by Polish Academy of SciencesThe authors thank Dr A.L. Goldstein for the gift of thymosin. Also are grateful to Dr J. Jaroszewski for helpful discussion.