The free radical oxidation of the tetrahydrocannabinols

Summary The free radical oxidation of ¹ and ⁶-tetrahydrocannabinol has been examined by spin trapping techniques and intermediates that would lead to cannabinol have been trapped. The 1st step in the oxidation of ¹-THC involves the removal of 3-H, while for ⁶-THC, either 2-H or 5-H. Intermediates were isolated which could be pyrolysed to the diene and cannabinol.     

The activity of monoamine oxidases A and B in gamma-irradiated rabbit brains

Summary The activities of monoamine oxidases A and B towards 5-hydroxytryptamine and -phenethylamine, respectively, were compared in the left and right caudatus, hippocampus, parietal cortex, cerebellum and frontal cortex 6 months after gamma-irradiation (single dose of 23 Gy) of either the right hemisphere or of the whole rabbit brain (in which case, a dose of 16 Gy). No difference in monoamine oxidase A or B activities were found in any of the brain regions.Acknowledgment. This study was supported by grants from the Swedish Medical Research Council (12x–5664 and 04x–4145), Hansson's, Lion's, Mångberg's and Sundblad's funds.     

Leydig cell andΔ 5-3β-hydroxysteroid dehydrogenase activity in the testis of toad (Bufo melanostictus) following cold exposuredehydrogenase activity in the testis of toad (Bufo melanostictus) following cold exposure

Summary Leydig cell nuclear area and ⁵-3-hydroxysteroid dehydrogenase activity were increased in the testis ofBufo melanostictus 2 days after a single short-term cold exposure. Both parameters returned to normal values 4 days later.     

Production and detection of antibody against estradiol receptor in the calf uterus. Interaction with the estrogen receptor from the hen oviduct]

The antibodies against estrogen receptor were obtained after injecting Rabbits with a cytoplasmic receptor fraction isolated from Calf uterus. The estrogen receptor was partially proteolysed by the action of trypsin and subsequently purified by affinity chromatography (purification 4,000 to 10,000 fold, to a purity of 5-20%). The affinity of the antibody for the proteolysed receptor is KD approximately 1 nM and serum titres have reached values of approximately 50 nM. The values remained constant after the third injection. Preliminary results indicate that the antibody has approximately the same affinity for "native" cytoplasmic estrogen receptor from Calf uterus, as well as for the "trypsinized" forms of estrogen receptor isolated from Calf uterine cytosol and Hen oviduct nuclei.     

Comparative study, in the Allium test, of the cytotoxicity of tubulosine and of 6 synthetized pseudo-tubulosines]

Comparing cytotoxicity of tubulosine and of six newly synthesized pseudo-tubulosines in the Allium test, we have characterized all the current features of protein inhibition. Nevertheless cytotoxicity of the six pseudotubulosines is much weaker. Furthermore, "floating poles anaphasis", a disturbance characteristic of the action of tubulosine, has been found with a lesser intensity with the other compounds.     

Field bioassay of male Douglas-fir beetle compound 3-methylcyclohex-3-en-1-one

Summary Synthetic 3-methylcyclohex-3-en-1-one, or 3,3-MCH, decreased by 77% the flight aggregation ofDendroctonus pseudotsugae (Scolytidae) to sticky traps containing attractant (frontalin, -pinene, and resin). However, windthrown trees were protected by 3,3-MCH much less than is known to occur with the isomer 3,2-MCH.This research was supported in part by NSF grants BMS 72-02522 and DEB 77-11361 and the O.S.U. School of Forestry. We thank the administration of the Siuslaw National Forest for study sites, and Dr L.M. Libbey for GC/MS of 3,3-MCH. Oregon State University Ag. Exp. Sta. Tech. Paper No. 4313.     

Effects of several preoperative medications on fat cell lipolysis, and activity of adipose tissue cyclic AMP phosphodiesterase.

Effects were examined of atropine, diazepam, pethidene, promethazine, scopolamine, omnopon and papaverine on basal and noradrenaline-stimulated lipolysis in rat isolated fat cells and on rat adipose tissue cyclic AMP phosphodiesterase activity. Papaverine at high concentration (1 mM) inhibited both basal and hormone-stimulated lipolysis, whereas diazepam enhanced basal lipolysis. At a 'clinical dose', omnopon increased both basal and noradrenaline-stimulated lipolysis. Adipose tissue cAMP phosphodiesterase activity was strongly inhibited by 1 mM diazepam, papaverine, promethazine and omnopon (280 microgram ml-1). Lack of enhancement of lipolysis by the established cAMP phosphodiesterase antagonist papaverine, is compatible with simultaneous inhibition also of adipose adenyl cyclase. Diazepam-stimulated lipolysis is compatible with its phosphodiesterase inhibitory activity. It is proposed that papaverine-containing omnopon may offer some survival advantages during surgical stress by facilitating a caloric supply.     

Influence of ethanol on thyroxine accumulation in the hypothalamus,pituitary gland and cerebrospinal fluid in the newborn rabbit

Summary The effect of ethanol on thyroxine (T₄) accumulation in the hypothalamus (H), pituitary gland (P) and cerebrospinal fluid (CSF) has been investigated in 1–15-day-old rabbits. It has been found that H or CSF serum ratios decreased with age by about 2 in the course of 13 postnatal days. Stable T₄ resulted in an increase of¹²⁵I-T₄ in H, P and CSF. Ethanol per se caused an increase in transfer and accumulation of radiothyroxine or made the changes after loading animals with carrier T₄ more pronounced.     

Mechanical responses of the isolated cervix of the day-22 pregnant rat to field stimulation.

Field stimulation of isolated, spirally-cut cervix from day-22 pregnant rats produced contractions which could be inhibited by tetrodotoxin or hyoscine and potentiated by propranolol. The rat cervix would appear to receive both cholinergic and noradrenergic innervations whose transmitters activate muscarinic cholinoceptors and beta-adrenoceptors respectively.