Dependence on pH of polarized sorting of secreted proteins

The plasma membranes of epithelial cells are divided into apical and basolateral domains. These two surfaces are characterized by markedly different protein compositions, reflecting the ability of the cell to target newly synthesized membrane proteins to specific regions of the cell surface. This targeting capability is also apparent in the polarized release of secretory products. Recent studies using canine renal tubule (MDCK) cells have suggested that distinct sets of secretory proteins are released from their apical and basolateral poles. We report experiments designed to examine secretory protein sorting by MDCK cells. We have shown that secretion of basement membrane components (laminin and heparan sulphate proteoglycan (HSPG] takes place from the basolateral cell surface and that this polarized release results from active sorting. The sorting process which mediates this polarized secretion requires an acidic intracellular compartment. MDCK cells treated with NH4Cl to raise the pH of their intracellular compartments, secrete laminin and HSPG by a default pathway which leads to their release in roughly equal quantities into the medium of both the apical and basolateral compartments.     

Oximinoacids in the inorganic nitrogen metabolism

Zusammenfassung Es wird gezeigt, dass in Zellen vonCryptococcus albidus und in Kulturlösung vonEndomycopsis lipolytica die Oxime bis zur Mitte der exponentiellen Zuwachsphase in steigenden Konzentrationen vorkommen, während sie in Ammonium-kultivierten Zellen fehlen.     

Activation of protein kinase C potentiates isoprenaline-induced cyclic AMP accumulation in rat pinealocytes

The pineal gland has proven to be an excellent model for the study of adrenergic control systems. Noradrenaline, released from sympathetic nerve terminals in the pineal gland, regulates a large nocturnal increase in melatonin synthesis by stimulating the activity of arylalkylamine N-acetyltransferase (NAT, EC 2.3.1.87) 30-70-fold. An essential step in both the induction and maintenance of high NAT activity is an increase in intracellular cyclic AMP. Noradrenaline acts via beta-adrenoceptors to increase pineal cyclic AMP by activating adenylate cyclase, and the activation of pineal alpha 1-adrenoceptors potentiates beta-adrenergic stimulation not only of NAT but of both cyclic AMP and cyclic GMP. Here we describe investigations designed to test whether alpha 1-adrenergic potentiation of beta-adrenergic stimulation of pineal cyclic AMP involves protein kinase C. Our results suggest that kinase activation is involved and the data provide the first demonstration of a synergistic interaction between Ca2+-phospholipid-dependent protein kinase (protein kinase C) and neurotransmitter-dependent stimulation of cyclic AMP.     

Note on the occurrence of Siphlonurus autumnalis (Ephemeroptera: Siphlonuridae) in a Montana spring brook

The little-known mayfly Siphlonurus autumnalis McDunnough (Ephemeroptera: Siphlonuridae) was collected from several locations in its floodplain habitats along the Middle Fork of the Flathead River in western Montana. To confirm identification, nymphs were reared along with an associated species, S. occidentalis Eaton (Ephemeroptera: Siphlonuridae). Adults emerged from 6 September until 16 October. Habitats are described and the first photos of the 2 sexes are provided.     

Nutrient relationships between Orobanche fasciculata Nutt. and its host Artemisia pygmaea Gray in the Uinta Basin of Utah, USA

Patterns of mineral nutrient uptake and distribution within the roots, stems, and leaves of Artemisia pygmaea and in the vascular parasite Orobanche fasciculata were investigated. All nutrients studied were magnified over concentrations found in the soil into the host and parasite. Nitrogen, phosphorus, potassium, and zinc were magnified along the flow gradient of soil-roots-stems-leaves of the host. All others increased in the roots and then decreased in the stems and leaves. Orobanche fasciculata concentrated phosphorus, potassium, and sodium over soil and root concentrations while excluding to some degree all others.     

Cattail decline at Farmington Bay Waterfowl Management Area

For the last seven years, 1969 to 1976, a steady decline of cattails ( Typha latifolia L.) has been observed at Farmington Bay Waterfowl Management Area, Davis County, Utah. Several parameters of Farmington Bay's environment that could cause or enhance a decline of the marshland vegetation were studied. These parameters included temperature, pH, phenols, oil and grease, heavy metals, fungus pathogens, and salt water intrusion from the Great Salt Lake. Elevated soluble salt concentrations were found to be responsible for the decline. Cattails are weakened or killed when they are exposed to soluble salt concentrations greater than 5.0 gm/liter, and there is a significant (1 percent level) negative correlation ( - 0.68) between soluble salt concentration and cattail height. Symptoms of elevated salt concentrations include stunted growth, leaf tip necrosis (burning), and occasional browning of an entire cattail clone. Furthermore, those salt concentrations that cause physiological stress in cattails also facilitate the growth of a decomposition fungus, Chaetophoma confluens. This fungus causes a rot consisting of irregularly scattered lesions on the surface of the rhizomes and was consistently isolated from rhizomes of declining plants.