Gene silencing in cancer by histone H3 lysine 27 trimethylation independent of promoter DNA methylation

Epigenetic silencing in cancer cells is mediated by at least two distinct histone modifications, polycomb-based histone H3 lysine 27 trimethylation (H3K27triM) and H3K9 dimethylation. The relationship between DNA hypermethylation and these histone modifications is not completely understood. Using chromatin immunoprecipitation microarrays (ChIP-chip) in prostate cancer cells compared to normal prostate, we found that up to 5% of promoters (16% CpG islands and 84% non-CpG islands) were enriched with H3K27triM. These genes were silenced specifically in prostate cancer, and those CpG islands affected showed low levels of DNA methylation. Downregulation of the EZH2 histone methyltransferase restored expression of the H3K27triM target genes alone or in synergy with histone deacetylase inhibition, without affecting promoter DNA methylation, and with no effect on the expression of genes silenced by DNA hypermethylation. These data establish EZH2-mediated H3K27triM as a mechanism of tumor-suppressor gene silencing in cancer that is potentially independent of promoter DNA methylation.     

Management Factors Influencing Location Selection Decisions of Independent Filmmakers: An Exploratory Case Study

This is an exploratory case study of a management issue that confronts filmmakers: how do they select locations for their films? The outcome of those decisions can have vast and immediate effects upon the profitability of a film. This is a sometimes shadowy, but significant industry with a major economic impact. For systems theory advocates, students, and explorers, this study demonstrates that investigative, immersive action research techniques can be used to explore what are otherwise hidden, somewhat cloistered business organizations and industry structures that are ordinarily closed to outsiders. The soft-system of this industry that is rooted upon trust, loyalty, alliances, relationships, and other non-financial bonds influence its decisions, and its institutional performance. While this research is focused upon a fragment of the overall film industry worldwide, and is thus limited in the universality of the propositions that subsequently emerge, this study can serve as a springboard for research into other soft systems, from which new knowledge of managerial perspectives can emerge.     

刺五加对清除氧自由基的作用

氧自由基是机体正常代谢的中间产物。心肌细胞缺血损伤时,可产生大量的氧自由基。超氧化物歧化酶(SOD)是氧自由基的清除剂。本文通过测定SOD活性,发现豚鼠于静滴哇巴因中毒出现室早时,SOD活性有明显降低。给予静注刺五加后,SOD活性迅速提高,与对照组比较,有非常显著的差异(P<0.01)。     

稀士元素钇对NbTi50wt%超导合金塑性影响研究

1 样品制备样品分两组:第一组研究添加 Y 的不同含量对合金塑性的影响,选用0.1,0.2,0.3wt%添加量,在预抽真空为10~(-1)mmHg 后充以工业氩气(纯度为98%)保护下,在水冷铜坩埚内反复熔炼成小钮扣锭.第二组样品是在真空度为10~(-4)mmHg 的自耗电弧炉中熔炼而成,一种添加0.3wt%Y,另一种是纯 NbTi50wt%.熔炼电压30kV,电流密度5A/mm~2.所用原料为标准国产 NbTi50wt%棒和纯度为99.95%的块状 Y.所有样品都经过1350℃、5小时均匀化热处理,950℃、20min 固溶处理,热锻,水淬(高于800℃),然后作拉伸试验.     

异步电动机球轴承的状态维修与剩余寿命预估

本文通过在计算机上用作图的方法，对大量轴承故障等级发展现场数据的分析，建立了球轴承等级ＡｄＢ发展的随机过程数学模型；研究该随机过程的均值函数和均方差函数，提出了电动机球轴承剩余寿命预估的方差曲线的近拟折一法；以及均值曲线趋势外法，实际应用证明，这两种方法均可用于预估球轴承利余使用寿命。这种剩余寿命预估的技术用于工厂设备维护，具有较大的经济价值。     

毛冬青甲素对心脏功能和血流动力学的作用

在整体猫心脏实验表明毛冬青甲素能增强心叽收缩性,增加心输出量、动脉血压和外周血管阻力,轻度减慢心率,增加分钟张力——时间指数,以低效率的方式增加心脏作功;轻度改善心脏的舒张特性。离体豚鼠心脏制备也表明毛冬青甲素增强心脏收缩;该药的量效曲线与异丙肾上腺素的量效曲线不平行,且不受心得安(10~(-7)M)的影响而右移,提示该药的正性肌力作用可能是对心肌直接作用,并可能不是通过β受体而实现。毛冬青甲素与哇巴因合用,不影响豚鼠哇巴因LD100。本文报导了一种利用(dp/dt)/P曲线测取左室等容舒张期压力降低的时间常数(T)的简便可行方法。     

群和线性半群的准完备性

本文提出了群完备和准完备的充要条件。同时,还较详尽地讨论了线性半群中的完备性问题。定出其所有的准完备类(即极大封闭集)     

最小二乘配点法解壳体弯曲问题

本文用最小二乘配点法分析弹性壳体弯曲问题。采用了加权残数法中的混合法—事先既不满足壳体弯曲定解微分方程式亦不满足边界条件,所选试函数为文献[1]中提到的双重幂级数。对于4边简支圆柱壳,其数值计算解与经典解析解误差不超过1.5％;对于悬臂圆柱壳,取其特例一悬臂效分析时,其结果与解析解误差亦不大。用本法可以编制出壳体弯曲问题的通用计算程序。