Inhibition of noradrenaline release from sympathetic nerves by pentobarbital

Summary Pentobarbital concentrations of 10–100 M selectively inhibited the noradrenaline release evoked by activation of the nicotinic receptors on the terminals sympathetic nerves of the rabbit heart. Higher concentrations also decreased the noradrenaline release induced by KCl or by electrical stimulation of the nerve axons.This paper is dedicated to Professor Dr. G. Malorny on the occasion of his 65th birthday.Acknowledgment: We thank Mrs G. Thielecke for technical assistance.     

Effect of beta-adrenergic blockade on intramuscular triglyceride mobilization during exercise

Summary It was shown in the rat that mobilization of intramuscular triglyceride during exercise is controlled by the adrenergic system.Acknowledgments. The work was supported by the Polish Academy of Sciences, grant No. 10.4.2.01.3.2.     

Striated inclusions and defective mitochondria in the restricted form of the ‘amycelial’ mutant ofNeurospora crassa

Summary The cytoplasm of the restrictive form of the amycelial mutant ofNeurospora crassa, growing on sucrosemedium, contains paracrystalline, microfilamentous inclusions and mitochondria with internal membranous whorls lacking in acetate-grown cultures.The support of the Fonds national suisse de la recherche scientifique is gratefully acknowledged.     

An assessment of the effect of hormone and neurotransmitters on adenine and gluanine derivatives simultaneously in rat brain cortical slices

Summary A 2-dimensional thin-layer method has been developed for the separation on cellulose of adenine and guanine derivatives. Using incubated rat cerebral cortex slices it was shown that noradrenaline and acetylcholine stirnulated cAMP and cGMP production respectively but glutamate and -aminobutyric acid stimulated production of both cyclic nucleotides.     

The effect ofγ-irradiation on soil enzyme stability

Summary Arylsulphatase, -1,3 glucanase, phosphatase and urease responded differently to -irradiation (5–50 Mrad) in air-dried and moist soils. In all instances phosphatase was the most stable. The variability between enzymes may be due to inherent biochemical and stuctural characteristics or to their location within the soil microenvironment.Supported by a grant from the NERC.     

Large-scale purification of hepatitis B surface antigen using affinity chromatography

Summary Large-scale purification of hepatitis B surface antigen, applicable to the preparation of potential vaccines for prevention of hepatitis B, is described. The method involves the following steps: precipitation of serum with polyethylene glycol 6000, affinity chromatography on concanavalin A-Sepharose and on -aminononyl-Sepharose, and isopycnic centrifugation.This study was supported by grant 09011 from the National Heart, Lung and Blood Institute. We thank Dr. B. Hollinger for testing HB_sAg samples by a complement fixation test.     

The behavioral development of the mutant "staggerer" mouse]

The behavioural study, in particular rearing environmental conditions, of the mutant mouse staggerer has shown that such animals may live more than 90 days. (he behavioural diagnosis of this mutation has been possible from the second week of life, using specific tests. A typical "bat posture" permits one to recognize the mutant from the normal Mouse. Locomotory and feeding behaviours also present late and various qualitatige particularities.     

New toxins from Aspergillus fumigatus Fresenius]

A strain of Aspergillus fumigatus Fres., isolated from sugar-beet draffs, synthesizes in vitro four toxic metabolites which have not yet been described in these fungal species. Toxic effects of the most abundant of these metabolites "fumitoxin A" have been studed on chick embryo. Artemia salina larvae, cell cultures, and on mice.     

Effect of cortisone or triiodothyronine administration to pregnant rats on lysosomal hydrolases in fetal forebrain and cerebellum

Triiodothyronine injected daily to pregnant rats for the last week of gestation (50 microgram/100 g b.wt) increased the specific activities of 5 acid glycosidases in the fetal forebrain and cerebellum. Cortisone (50 mg/100 g b.wt) administered in the same period had no effect on cerebellum acid hydrolases, but decreased their activity in the forebrain.