反应器类型对好氧颗粒污泥形成与运行稳定性的影响试验分析

为探讨如何能快速形成好氧颗粒污泥,在不同的SBR反应器内分别加设网板直径为16cm和6cm,孔径为8mm,孔间距为1mm的水平网板,并在此基础上改变反应器的高径比(H/D),分析了网板及H/D对好氧颗粒污泥快速颗粒化的影响.试验结果表明:在反应器加设网板的条件下,H/D≥75∶7时可培养出好氧颗粒污泥,而H/D=1∶1时不能形成好氧颗粒污泥.     

浅析鸡西矿区矿井水利用及发展前景

根据国家产业政策的要求,结合当地实际情况,产述矿井水产生的原因、种类及矿井水处理后的利用途径,分析鸡西矿区矿井水利用及发展前景,矿井水的利用既保护淡水资源,又实现节能降耗减排,从而促进水资源结构的优化。提高企业水资源利用率,这也是企业降低用水成本、提高经济效益、增强竞争力的有效措施。     

Diversity and high nitrogenase activity of endophytic diazotrophs isolated from Oryza rufipogon Griff.

Diversity and nitrogenase activity of endophytic diazotrophs colonized in the wild rice Oryza rufipogon Griff grown in Boluo, Huilai County in Guangdong Province and Lingshui County in Hainan Province were studied. Thirty-seven isolates obtained from Oryza rufipogon were identified as putative endophytic nitrogen-fixing bacteria by ARA （acetylene reduction assay） test and further confirmed by PCR amplification of nifH gene fragments. All obtained strains have ARA activity and the same sized nifH gene fragments. Above the similarity level of 80%, the obtained isolates were assigned as Group Ⅰ to Ⅷ by the clustering of IS-PCR fingerprints. The SDS-PAGE whole-cell protein patterns were similar to those of IS-PCR fingerprints. Components and contents of fatty acid methyl esters （FAMEs） were used to differentiate the representative strains （Ls13, Ls8, BL1, BL12, HL6, Ls4） from Group Ⅰ to Group Ⅵ. The six representative strains showed significant difference in contents and components of cellular fatty acid methyl ester. 16S rDNA sequencing analysis showed that strains of Group Ⅰ to Ⅶ were located in Enterobacteraceae （y-proteobacteria）. Strains of Group Ⅰ and Group Ⅱ were closely related to Klebsiella sp.; Strain Ls8 of Group Ⅱ was a little far away from the genus of Pantoea （homology level 96% with Pantoea agglomerans）, which may represent a new species or genus in Enterobacteraceae; Strains of Groups Ⅳ and Ⅴ belonged to different Enterobacter sp.; Strain Ls4 and Ls 9 representing Group Ⅵ were close to Citrobacter amalonaticus with 98% sequence similarity; Strain Ls15 of Group Ⅶ showed 98% sequence identity with Pantoea sp.; Strains of Group Ⅷ were assigned to the genus Ideonella （β-proteobacteria）. Based on the above results, endophytic diazotrophs isolated from O. rufipogon showed great diversity and some diazotrophs showed high nitrogenase activity with 42.52 μmol/mL. h C2H4. Inoculation to rice tests indicated that the isolated endophytic diazotrophs significantly promoted the rice growth.     

Microwave-assisted ring-opening copolymerization of ε-caprolactone and 2-Phenyl-5,5-bis（oxymethyi） trimethylene carbonate

A series of polycarbonate copolymers were synthesized by microwave-assisted ring-opening polymerization of ε-caprolactone （CL） and 2-phenyl-5,5-bis（oxymethyl） trimethylene carbonate （PTC） with Tin （Ⅱ） 2-ethylhexanoate as a catalyst. These copolymers obtained were detected by 1H NMR, FT-IR, UV, gel permeation chromatography （GPC）, differential scanning calorimetry （DSC） and automatic contact angle meter. The influences of the feed molar ratio of monomers, catalyst concentration, reaction time and reaction temperature, as well as microwave irradiation power on the copolymerization process were also studied. In vitro degradation tests indicated that these polycarbonate copolymers possess the slow degradation rates and strong hydrophobicity. In vitro release profiles of 5-Fu from copolymers showed that copolymers have the steady drug release rates and good controlled release properties.     

基于遗传算法的多agent规划协调研究

在多agent问题求解中,关键是如何规划各agent的活动,当问题非常复杂时,一般规划中所采用的经典搜索算法效率不高.在前人工作基础上,结合遗传算法理论.对于周围世界是部分可观察的或非确定性的多agent系统,给出一种改进的基于遗传算法的多agent协调规划算法,并分析了多agent协调规划中的通信技术,给出一种多agent系统的通信模型.     

高校师范类女大学生运动健身认知行为的研究

运用问卷调查法,文献综述法和数理统计法对高校师范类女大学生运动健身认知、行为的现状进行分析研究.研究结果表明:师范类女大学生对运动健身的"知、信、行"有明显的偏差.通过分析研究,建议在对师范类女大学生的运动健身活动给予适当的技术指导,促进女大学生体育运动健身以及健康的开展.     

条斑星鲽连续性鳍细胞系的建立与鉴定

为了建立条斑星鲽鳍细胞系,为其细胞工程及病毒学研究奠定基础,对经Ⅱ型胶原酶和透明质酸酶联合消化法所得鳍组织碎块分别用DMEM/F12、L-15和M199培养液(pH7.2)在18～26℃进行了鳍组织的体外培养,并在所筛选出的最适培养液和培养温度下通过添加羧甲基壳寡糖、碱性成纤维样生长因子(bFGF)和I型胰岛素样生长因子(IGF-I)启动了鳍细胞的原代培养。体外培养结果显示,条斑星鲽鳍细胞的最适培养液为DMEM/F12培养液,最适培养温度为22℃,原代培养鳍细胞的生长分裂状态旺盛,细胞形态主要为成纤维样,20d后便可形成汇合细胞单层,经过连续的继代培养已建立了条斑星鲽的连续性鳍细胞系,目前已传至第135代;生长特性检测结果显示,第60代鳍细胞系细胞的群体倍增时间为56.9h,其生长分裂状态依然十分旺盛;染色体分析结果显示,第60代鳍细胞系细胞虽然出现了染色体的非整倍性,但其特征性染色体数目仍为46条,并具有2sm+44t的正常二倍体核型,证明所建立的细胞系确为条斑星鲽连续性鳍细胞系。该细胞系为条斑星鲽的细胞工程育种和病毒-细胞相互作用提供了一个理想的体外研究体系,具有重要的理论意义和应用价值。     

羊场湾煤矿大断面巷道支护参数分析与选择

大断面巷道受赋存环境、开采扰动及人工支护的耦合影响,其稳定与否直接决定矿井的安全生产。以破碎围岩环境下羊场湾矿大断面巷道为背景,采用理论分析、FLAC3D数值模拟和锚杆测力计、压力表等现场监测方法,综合分析了锚杆受力和巷道变形情况,并定量确定了巷道的支护参数。实践表明:顶板和两帮锚杆(索)采用850mm×850mm,850mm×900mm的支护能够满足矿井安全开采的需要。     

Periodic flow characteristics during RH vacuum circulation refining

The circulation period of RH vacuum refining was studied to promote the refining efficiency. The influences of the lift gas flow rate and submersion depth of snorkels on the circulation period, and the relationship between mixing time and circulation flow were discussed. The effects of the lift gas flow rate and submersion depth on the degassing rate in one circulation period were studied by water modeling. The results show that the circulation period is shortened by increasing the lift gas flow rate. The circulation period is the shortest when the submersion depth of snorkels is 560 mm. The whole ladle can be mixed thoroughly after three times of circulation. Increasing the lift gas flow rate can enhance the degassing rate of RH circulation.     

基于约束规划的小天体接近段鲁棒制导控制方法

以小天体接近段任务为背景,提出了一种基于约束规划的鲁棒制导控制方法。该方法将目标天体引力模型引入轨迹规划过程中,并将具有控制约束与轨迹状态约束的非线性动力学路径规划问题转化为以优化燃耗为性能指标的二阶圆锥规划(second order cone program, SOCP)问题|同时考虑目标天体引力模型与外界干扰等不确定因素对轨迹跟踪的影响,基于模型不确定界设计鲁棒反馈控制律。数学仿真证明,在相同条件下,基于凸规划的制导控制性能无论在制导控制偏差或是状态与控制约束满足方面均优于传统多项式拟合制导控制算法。