全文获取类型
收费全文 | 12153篇 |
免费 | 123篇 |
国内免费 | 143篇 |
专业分类
系统科学 | 743篇 |
丛书文集 | 821篇 |
教育与普及 | 573篇 |
理论与方法论 | 55篇 |
现状及发展 | 996篇 |
研究方法 | 1323篇 |
综合类 | 7897篇 |
自然研究 | 11篇 |
出版年
2018年 | 22篇 |
2017年 | 31篇 |
2016年 | 39篇 |
2015年 | 33篇 |
2014年 | 61篇 |
2013年 | 59篇 |
2012年 | 661篇 |
2011年 | 840篇 |
2010年 | 255篇 |
2009年 | 61篇 |
2008年 | 774篇 |
2007年 | 795篇 |
2006年 | 986篇 |
2005年 | 993篇 |
2004年 | 789篇 |
2003年 | 720篇 |
2002年 | 665篇 |
2001年 | 542篇 |
2000年 | 764篇 |
1999年 | 269篇 |
1998年 | 78篇 |
1997年 | 49篇 |
1996年 | 50篇 |
1995年 | 56篇 |
1994年 | 64篇 |
1993年 | 84篇 |
1992年 | 48篇 |
1991年 | 72篇 |
1990年 | 49篇 |
1989年 | 60篇 |
1988年 | 48篇 |
1987年 | 52篇 |
1986年 | 68篇 |
1985年 | 69篇 |
1984年 | 64篇 |
1983年 | 60篇 |
1982年 | 68篇 |
1981年 | 48篇 |
1980年 | 26篇 |
1979年 | 34篇 |
1971年 | 34篇 |
1970年 | 61篇 |
1966年 | 21篇 |
1959年 | 206篇 |
1958年 | 369篇 |
1957年 | 271篇 |
1956年 | 221篇 |
1955年 | 219篇 |
1954年 | 249篇 |
1948年 | 76篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
91.
孟小璐 《重庆工商大学学报(自然科学版)》2018,35(4):44-51
针对成本在经济学系统中变化的非对称,与其影响因素之间的非线性关系,提出采用神经网络分位数回归法来研究成本与各影响因素之间的联系,并进行成本预测;该方法不仅可以通过神经网络结构模拟经济系统中非线性关系,还可以通过分位数回归功能揭示各影响因素对成本整个条件分布的影响规律;通过实证结果分析,神经网络分位数回归模型相较于OLS回归模型和分位数回归模型其预测精度更高,且揭示了各因素的影响规律,所以神经网络分位数回归模型的分析结果更科学,更有价值,更有助于相关管理决策者进行成本分析、控制和管理。 相似文献
92.
The pedigrees of three sequenced rice cultivars were analyzed to show that a majority of the genetic composition of 'Nipponbare' originates from japonica cultivars while the minority originates from indica cultivars. In contrast, '93-11' is derived mainly from indica cultivars with a smaller contribution from japonica cultivars. All ancestors of 'Guang lu ai 4' appeared to be indica lines. A set of molecular markers (46 InDels and 53 SSRs) polymorphic between 'Nipponbare' and '93-11' were examined in 46 typical indica and 47 typical japonica cultivars selected from 443 accessions according to Cheng's index. All cultivars were divided into indica and japonica groups without overlapping when clustered by Cheng's index, InDels and SSRs. Much higher InDel and SSR diversity between groups than within groups implies that the marker polymorphisms between 'Nipponbare' and '93-11' represent a large proportion of inter-subspecific diversity. About 85% of indica cultivars and more than 90% of japonica cultivars were confirmed to have the same PCR banding patterns as '93-11' and 'Nipponbare', respectively. Some polymorphic loci between 'Nipponbare' and '93-11' cannot be validated in other indica and japonica cultivars, either as subspecies-specific but not predominant alleles, or alleles not specific between the two groups. It was concluded that molecular markers developed from sequence polymorphism between 'Nipponbare' and '93-11' often represent inter-subspecific diversity, although some exceptions were sensitive to either particular marker loci or particular cultivars. 相似文献
93.
94.
Nuclear transfer into mouse zygotes 总被引:6,自引:0,他引:6
95.
Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency 总被引:13,自引:0,他引:13
Irwin WA Bergamin N Sabatelli P Reggiani C Megighian A Merlini L Braghetta P Columbaro M Volpin D Bressan GM Bernardi P Bonaldo P 《Nature genetics》2003,35(4):367-371
Collagen VI is an extracellular matrix protein that forms a microfilamentous network in skeletal muscles and other organs. Inherited mutations in genes encoding collagen VI in humans cause two muscle diseases, Bethlem myopathy and Ullrich congenital muscular dystrophy. We previously generated collagen VI-deficient (Col6a1-/-) mice and showed that they have a muscle phenotype that strongly resembles Bethlem myopathy. The pathophysiological defects and mechanisms leading to the myopathic disorder were not known. Here we show that Col6a1-/- muscles have a loss of contractile strength associated with ultrastructural alterations of sarcoplasmic reticulum (SR) and mitochondria and spontaneous apoptosis. We found a latent mitochondrial dysfunction in myofibers of Col6a1-/- mice on incubation with the selective F1F(O)-ATPase inhibitor oligomycin, which caused mitochondrial depolarization, Ca2+ deregulation and increased apoptosis. These defects were reversible, as they could be normalized by plating Col6a1-/- myofibers on collagen VI or by addition of cyclosporin A (CsA), the inhibitor of mitochondrial permeability transition pore (PTP). Treatment of Col6a1-/- mice with CsA rescued the muscle ultrastructural defects and markedly decreased the number of apoptotic nuclei in vivo. These findings indicate that collagen VI myopathies have an unexpected mitochondrial pathogenesis that could be exploited for therapeutic intervention. 相似文献
96.
Chiang C Jacobsen JC Ernst C Hanscom C Heilbut A Blumenthal I Mills RE Kirby A Lindgren AM Rudiger SR McLaughlan CJ Bawden CS Reid SJ Faull RL Snell RG Hall IM Shen Y Ohsumi TK Borowsky ML Daly MJ Lee C Morton CC MacDonald ME Gusella JF Talkowski ME 《Nature genetics》2012,44(4):390-7, S1
We defined the genetic landscape of balanced chromosomal rearrangements at nucleotide resolution by sequencing 141 breakpoints from cytogenetically interpreted translocations and inversions. We confirm that the recently described phenomenon of 'chromothripsis' (massive chromosomal shattering and reorganization) is not unique to cancer cells but also occurs in the germline, where it can resolve to a relatively balanced state with frequent inversions. We detected a high incidence of complex rearrangements (19.2%) and substantially less reliance on microhomology (31%) than previously observed in benign copy-number variants (CNVs). We compared these results to experimentally generated DNA breakage-repair by sequencing seven transgenic animals, revealing extensive rearrangement of the transgene and host genome with similar complexity to human germline alterations. Inversion was the most common rearrangement, suggesting that a combined mechanism involving template switching and non-homologous repair mediates the formation of balanced complex rearrangements that are viable, stably replicated and transmitted unaltered to subsequent generations. 相似文献
97.
Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes 总被引:56,自引:0,他引:56
Double-stranded RNA interference (RNAi) is an effective method for disrupting expression of specific genes in Caenorhabditis elegans and other organisms. Applications of this reverse-genetics tool, however, are somewhat restricted in nematodes because introduced dsRNA is not stably inherited. Another difficulty is that RNAi disruption of late-acting genes has been generally less consistent than that of embryonically expressed genes, perhaps because the concentration of dsRNA becomes lower as cellular division proceeds or as developmental time advances. In particular, some neuronally expressed genes appear refractory to dsRNA-mediated interference. We sought to extend the applicability of RNAi by in vivo expression of heritable inverted-repeat (IR) genes. We assayed the efficacy of in vivo-driven RNAi in three situations for which heritable, inducible RNAi would be advantageous: (i) production of large numbers of animals deficient for gene activities required for viability or reproduction; (ii) generation of large populations of phenocopy mutants for biochemical analysis; and (iii) effective gene inactivation in the nervous system. We report that heritable IR genes confer potent and specific gene inactivation for each of these applications. We suggest that a similar strategy might be used to test for dsRNA interference effects in higher organisms in which it is feasible to construct transgenic animals, but impossible to directly or transiently introduce high concentrations of dsRNA. 相似文献
98.
Mutations in the gene encoding pejvakin, a newly identified protein of the afferent auditory pathway, cause DFNB59 auditory neuropathy 总被引:5,自引:0,他引:5
Delmaghani S del Castillo FJ Michel V Leibovici M Aghaie A Ron U Van Laer L Ben-Tal N Van Camp G Weil D Langa F Lathrop M Avan P Petit C 《Nature genetics》2006,38(7):770-778
Auditory neuropathy is a particular type of hearing impairment in which neural transmission of the auditory signal is impaired, while cochlear outer hair cells remain functional. Here we report on DFNB59, a newly identified gene on chromosome 2q31.1-q31.3 mutated in four families segregating autosomal recessive auditory neuropathy. DFNB59 encodes pejvakin, a 352-residue protein. Pejvakin is a paralog of DFNA5, a protein of unknown function also involved in deafness. By immunohistofluorescence, pejvakin is detected in the cell bodies of neurons of the afferent auditory pathway. Furthermore, Dfnb59 knock-in mice, homozygous for the R183W variant identified in one DFNB59 family, show abnormal auditory brainstem responses indicative of neuronal dysfunction along the auditory pathway. Unlike previously described sensorineural deafness genes, all of which underlie cochlear cell pathologies, DFNB59 is the first human gene implicated in nonsyndromic deafness due to a neuronal defect. 相似文献
99.
Purification and cloning of amyloid precursor protein beta-secretase from human brain 总被引:40,自引:0,他引:40
Sinha S Anderson JP Barbour R Basi GS Caccavello R Davis D Doan M Dovey HF Frigon N Hong J Jacobson-Croak K Jewett N Keim P Knops J Lieberburg I Power M Tan H Tatsuno G Tung J Schenk D Seubert P Suomensaari SM Wang S Walker D Zhao J McConlogue L John V 《Nature》1999,402(6761):537-540
Proteolytic processing of the amyloid precursor protein (APP) generates amyloid beta (Abeta) peptide, which is thought to be causal for the pathology and subsequent cognitive decline in Alzheimer's disease. Cleavage by beta-secretase at the amino terminus of the Abeta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment. Cleavage of the C-terminal fragment by gamma-secretase(s) leads to the formation of Abeta. The pathogenic mutation K670M671-->N670L671 at the beta-secretase cleavage site in APP, which was discovered in a Swedish family with familial Alzheimer's disease, leads to increased beta-secretase cleavage of the mutant substrate. Here we describe a membrane-bound enzyme activity that cleaves full-length APP at the beta-secretase cleavage site, and find it to be the predominant beta-cleavage activity in human brain. We have purified this enzyme activity to homogeneity from human brain using a new substrate analogue inhibitor of the enzyme activity, and show that the purified enzyme has all the properties predicted for beta-secretase. Cloning and expression of the enzyme reveals that human brain beta-secretase is a new membrane-bound aspartic proteinase. 相似文献
100.
<正> 在3、4月间,对产后20—31天正在泌乳的母羊诱导发情。发情时配种,配种后11、12和13天,用一种盐水或绒毛膜促性腺激素处理母羊(HCG;100国际单位)。对经过妊娠诊断的母羊,不论妊娠或未妊娠,发现配种后12、14和16天,用绒毛膜促性腺激素处理过的比用盐水处理过的血清中孕酮浓度增加(P<0.01)。用绒毛膜促性腺激素处理的母羊比用盐水(29%58标准液;P<0.05)处理的母羊妊娠率较高。从试验提供的资料指出,用绒毛膜 相似文献