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231.
J. W. M. Lagerberg J. VanSteveninck T. M. A. R. Dubbelman 《Cellular and molecular life sciences : CMLS》1997,53(3):257-262
The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in
the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed
membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase
of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H2O2 exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed
increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a
slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed
MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant
BHT in the incubation medium. However, open ghosts exposed to H2O2 showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission
spectra and gel filtration studies showed that MC540 can bind to H2O2-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H2O2 revealed that peroxidation of lipids with H2O2 induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively
denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer
leaflet of the membrane based on increased MC540-staining should be drawn with care.
Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996 相似文献
232.
Two classes of metabolites fromTheonella swinhoei are localized in distinct populations of bacterial symbionts 总被引:7,自引:0,他引:7
C. A. Bewley N. D. Holland D. J. Faulkner 《Cellular and molecular life sciences : CMLS》1996,52(7):716-722
The marine spongeTheonella swinhoei (lithistid Family Theonellidae, Order Astrophorida) has yielded many important, bioactive natural products, most of which share structural features with bacterial natural products. The presence of microbial symbionts inT. swinhoei has been reported, and it was originally suggested that the cytotoxic macrolide swinholide A and many of the bioactive cyclic peptides fromT. swinhoei were all produced by symbiotic cyanobacteria. By transmission electron microscopy, we found four distinct cell populations to be consistently present inT. swinhoei: eukaryotic sponge cells, unicellular heterotrophic bacteria, unicellular cyanobacteria and filamentous heterotrophic bacteria. Purification and chemical analyses of each cell type showed the macrolide swinholide A to be limited to the mixed population of unicellular heterotrophic bacteria, and an anti-fungal cyclic peptide occurred only in the filamentous heterotrophic bacteria. Contrary to prior speculation, no major metabolites were located in the cyanobacteria or sponge cells. 相似文献
233.
A. A. Shaheen A. A. Abd El-Fattah M. Z. Gad 《Cellular and molecular life sciences : CMLS》1996,52(4):336-339
The level of malondialdehyde (MDA), an index of lipid peroxidation, and the antioxidants superoxide dismutase (SOD) and glutathione (GSH), as well as the activity of Na+, K+-ATPase, were assessed in whole rat brain after immobilization, anemic hypoxia (NaNO2) and 72 h starvation. The effect of these stressors on plasma glucose and corticosterone levels was also observed. Hypoxia and starvation stimulated the lipidj peroxide formation in braini as indicated by an increase in the level of MDA, being higher after starvation than hypoxia. Brain SOD activity was also increased in response to hypoxia and starvation while GSH content was only diminished ini hypoxia. However, neither MDA nor antioxidants were affected by immobilization. On the other hand, the activity of brain Na+, K+-ATPase was significantly increased by immobilization and hypoxia but decreased in starvation. A similar pattern of change was also observed in plasma glucose and corticosterone levels in response to these stressors. These results elucidate differences in the biochemical response of animals towards various types of stress, with increased lipid peroxide formation in hypoxia and starvation. 相似文献
234.
论草原生态系统中微气候研究的重要作用 总被引:2,自引:0,他引:2
草原生态系统中微气候的研究工作开始于六七十年代,主要是应用微气候学和生态学研究草原生态系统中微气候的变化规律及其与动植物的关系。这些研究工作不但丰富了生态学理论,而且对草地生产有重要的指导意义。中国在这方面的研究起步晚,报道甚少。文章旨在论述微气候学研究在草原生态系统中的重要地位,推动中国在这一领域的发展。 相似文献
235.
目的:根据本病的特殊性和严重性,探索中西医结合治疗的新途径。方法:中医严格辨证用药。辅以西药治疗。结果:中药为主辅以西药治疗较单纯西医治疗效果明显。结论:认清病因,标本兼治,疗效显著。 相似文献
236.
本书是施普林格出版社2005年出版的一本关于螺旋压缩机的新著。螺旋式压缩机是一种最常见的用于压缩气体的机器,由于其构造简单、工作效率高而受到人们的关注。本书系统阐述了螺旋压缩机的种类、几何参数转子的设计工作原理和螺旋压缩机的计算。 相似文献
237.
张为人 《科技情报开发与经济》2004,14(6):168-169
根据智能住宅火灾形成的特点,简述了消火栓给水系统与自动喷水灭火系统的设置方式与特点,并结合实例对消防给水方式的选择等问题进行了探讨。 相似文献
238.
介绍了一种新型的指纹识别装置(晶体传感器的指纹识别装置),并借此分析和研究了指纹识别系统的识别原理和设计方法,这些原理和方法对指导和优化指纹识别系统的设计提供了依据。 相似文献
239.
张新 《科技情报开发与经济》2004,14(6):49-50
知识管理是知识经济时代的一种全新的管理思想。阐述了高校图书馆在对自身实施知识管理时应注意的环节、存在的问题以及所要避免陷入的几个误区,并对未来的知识管理进行了展望。 相似文献
240.
城市定位与城镇群建设 总被引:2,自引:0,他引:2
城市是一个国家和民族对外开放的窗口、商品国际交换的平台,它决定着整个国家和地区的竞争力。文章对廊坊市城市发展的现状和廊坊市城镇群建设战略进行了论述,希望对我国其他城市的发展有一定的参考作用。 相似文献