Stem cells find their niche.

The concept that stem cells are controlled by particular microenvironments known as 'niches' has been widely invoked. But niches have remained largely a theoretical construct because of the difficulty of identifying and manipulating individual stem cells and their surroundings. Technical advances now make it possible to characterize small zones that maintain and control stem cell activity in several organs, including gonads, skin and gut. These studies are beginning to unify our understanding of stem cell regulation at the cellular and molecular levels, and promise to advance efforts to use stem cells therapeutically.     

Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution.

Ion transport proteins must remove an ion's hydration shell to coordinate the ion selectively on the basis of its size and charge. To discover how the K+ channel solves this fundamental aspect of ion conduction, we solved the structure of the KcsA K+ channel in complex with a monoclonal Fab antibody fragment at 2.0 A resolution. Here we show how the K+ channel displaces water molecules around an ion at its extracellular entryway, and how it holds a K+ ion in a square antiprism of water molecules in a cavity near its intracellular entryway. Carbonyl oxygen atoms within the selectivity filter form a very similar square antiprism around each K+ binding site, as if to mimic the waters of hydration. The selectivity filter changes its ion coordination structure in low K+ solutions. This structural change is crucial to the operation of the selectivity filter in the cellular context, where the K+ ion concentration near the selectivity filter varies in response to channel gating.     

Vegetation characteristics of mountainous northeastern Nevada sagebrush community types

Vegetation characteristics of 15 sagebrush community types identified on the Humboldt National Forest, northeastern Nevada, are described. A total of 218 plant species were found over the 372 relatively undisturbed rangeland communities sampled. The dominant plant families encountered were the Asteraceae (45 taxa), Poaceae (32 taxa), Scrophulariaceae (14 taxa), and the Fabaceae (12 taxa). Average annual dry weight production of the community types ranged from about 400 kg/ha/yr on types with Artemisia nova as the dominant sagebrush species to 1,200 kg/ha/yr on some A. tridentata ssp. vaseyana community types. A general increase in species richness and vegetation plus litter ground cover was observed within community types as the dominant sagebrush species changed from A. nova to A. arbuscula to A. longiloba to A. tridentata spp. tridentata to A. tridentata ssp. vaseyana . Major differences in plant species production and constancy exist between the sagebrush community types studied.     

Body fat, body water, and total caloric value of Uinta ground squirrels

The weight of body water and fat-free dry weight of Uinta ground squirrels ( Spermophilus armatus ) is given as a function of body weight. Body fat is presented as a function of body weight and body length. An equation for calculating the total caloric content of Uinta ground squirrels is given. Fat index (weight of fat/fat-free dry weight) values of juveniles increased from 0.11 to 0.22 from 5 June to 28 July. In male yearlings and adults the fat index increased about 8 to 9 times in the latter half of June and early July.         

Catastrophic wildfire and number of populations as factors influencing risk of extinction for Gila trout ( Oncorhynchus gilae )

We used the computer program RAMAS to explore the sensitivity of an extinction-risk model for the Gila trout ( Oncorhynchus gilae ) to management of wildfires and number of populations of the species. The Gila trout is an endangered salmonid presently restricted to very few headwaters of the Gila and San Francisco river tributaries in southwestern New Mexico. Life history data for 10 extant populations were used to examine sensitivity of the species viability to changes in a variety of factors including population size, fecundity, life stage structure, number of populations, severity and probability of forest fires, and a regulated fishery. The probability and severity of forest fires and number of populations had the greatest effect on viability. Results indicate that successful conservation of Gila trout requires establishment of additional populations and reduction of the severity of forest fires through a program incorporating more frequent, but less severe, fires.     

Quadrat and sample sizes for frequency sampling mountain meadow vegetation

Proper quadrat and sample sizes for sampling species frequency vary among vegetation types. This study found 10 × 10-cm quadrats most appropriate for frequency sampling dry mountain meadows when using a single quadrat size. If large, broad-leaved forbs are of special interest, 25 × 25-cm, 25 × 50-cm, or 50 × 50-cm quadrats may be useful. One hundred 10 × 10-cm quadrats adequately sampled most common species at alpha = 0.20 ± 10% frequency. In contrast, more than five hundred 0.29-m 2 circular plots usually were needed to sample individual species yield at alpha = 0.20 ± 20% of the mean.     

Effects of elevation on litter-size variation among lizard populations in the Sceloporus grammicus complex (Phrynosomatidae) in Mexico

We examined the effect of elevation on litter-size variation in viviparous lizards of the Sceloporus grammicus complex in 10 states of Mexico. Female snout–vent length (SVL) decreased with increasing elevation, and absolute litter size based on embryos also tended to de crease with increasing elevation. However, after controlling for variation in female body size, we found that litter sizes tended to be relatively larger at higher elevation. Elevation therefore appears to influence litter size in these lizards; however, relatively little of the variation is explained by elevation; thus, other factors are likely making substantial contributions to the observed litter-size variation. The S. grammicus complex appears to be a good model system for examining the underlying causes of geographic and elevational variation in lizard life histories. Examinamos el efecto del altitud en la variación del tamaño de camada de las lagartijas vivíparas del complejo Sceloporus grammicus en 10 estados de México. La LHC de las hembras disminuyó con la altitud, y el tamaño absoluto de camada, calculado con base en el número de embriones, también tendió a disminuir. No obstante, después de controlar la variación en el tamaño corporal de las hembras, encontramos que los tamaños de camada tendieron a ser relativamente más grandes en altitudes mayores. La altitud, por tanto, parece influir en el tamaño de camada de estas lagartijas; sin embargo, la altitud explica relativamente poco de la variación, por lo que, es probable que otros factores contribuyan substancialmente a la variación observada en el tamaño de camada. El complejo S. grammicus parece ser un buen sistema para estudiar las causas fundamentales de la variación geográfica y altitudinal en la historia de vida de las lagartijas.     

Richard Watson

The development of the circular dividing engine in England is traced from Henry Hindley and Jesse Ramsden through the improvements introduced by Ramsden's successors to the self-acting engine of William Simms. Particular emphasis is given to the invention, evolution and transmission of the methods used to achieve accuracy in: dividing the wheel; ratching the teeth and matching them to the endless screw; and mounting the cutter. The procedures adopted by Ramsden and Troughton for correcting initial dividing marks are also described.     

Soil properties associated with vegetation patches in a Pinus ponderosa —bunchgrass mosaic

Since Euro-American settlement, fire exclusion and other factors have dramatically altered interior western coniferous forests. Once open and parklike, present-day structure in many southwestern Pinus ponderosa forests consists of dense stands of young, small-diameter trees, with small patches of larger, old trees, and relict open bunchgrass areas. Our objectives were to assess differences in soil properties associated with these different vegetation patches. We examined soil morphological characteristics, pH, organic C concentration, total N concentration, C:N ratio, and phytolith concentration from profiles within 6 transects (18 soil pedons) crossing patches of dense stands of small diameter trees, patches of old-growth trees, and open grassy areas. Results indicate that old-growth plots had significantly lower A horizon pH and thicker O horizons than grass plots. In general, we found vegetation patches had statistically similar C and N concentrations and C:N ratios for A and B horizons; however, C in the A horizon was positively correlated with O horizon accumulation ( r 2 = 0.79). Greater accumulation of organic C in the A horizon of forested areas contrasts with commonly reported results from mesic, mid-continental prairie-forest ecosystems but is typical for many arid, semiarid, and humid savanna ecosystems. Phytolith concentration was similar among old-growth pine, dense younger pine, and open grassy plots; the lack of a spatial pattern in phytolith distribution could indicate that grass cover was more spatially continuous in the past. Additionally, this interpretation is consistent with current theories regarding historical vegetation change in these forests.     

Occurrence of ticks on mule deer in central Utah

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