层次分析法在彩色钥匙销售预测中的应用

本文利用层次分析法对唐山地区的彩色钥匙销售市场进行了分析、预测。在分析了影响消费者购买彩色钥匙各项指标的基础上，将所有人群分为三种类型，对彩色钥匙的款式和图案等因素进行了分析。     

辱虐管理对知识型员工离职倾向的影响及其作用机制

以随机抽取的320名知识型员工为样本,依据社会交换和社会认同等理论,实证研究了中国情境下辱虐管理对知识型员工离职倾向的影响,并进一步分析了组织认同和人格特质所起的中介和调节作用.研究结果表明,辱虐管理与知识型员工的离职倾向存在着显著的正相关关系;组织认同在辱虐管理对知识型员工离职倾向的影响过程中起部分中介作用;人格特质会在一定程度上调节缓冲辱虐管理对知识型员工离职倾向的影响.因此,企业应加强制度和文化建设,通过减少领导的辱虐管理,增强员工的组织认同等,避免知识型员工离职倾向的产生.     

蓝莓籽不同组分抗氧化作用的比较

目的:为了加强蓝莓籽的综合利用开发,研究蓝莓籽不同组分的抗氧化能力。方法:对蓝莓籽的醇提取物、蓝莓籽多酚、蓝莓籽蛋白、蓝莓籽多糖进行提取分离,采用AAPH法、DPPH法对各组分进行抗氧化能力测定。结果:AAPH法测定的抗氧化能力由高到低的顺序为:蓝莓籽多糖﹥蓝莓籽蛋白≈α-VC﹥醇提取物﹥蓝莓籽多酚;DPPH法测定的清除自由基能力由高到低顺序为:蓝莓籽多酚﹥醇提取物≈α-VC﹥蓝莓籽蛋白﹥蓝莓籽多糖。结论:蓝莓籽不同组分均显示出一定的抗氧化能力,其中以蓝莓籽多糖、蓝莓籽蛋白的抗氧化能力最强,蓝莓籽多酚的清除自由基能力最强。     

不同厂家豆豉理化指标及降血压ACE抑制活性的测定与评价简

对3个厂家(Y1,Y2,Y3)生产的永川豆豉的理化指标、水提物血管紧张素转化酶(ACE)抑制活性等指标进行测定与评价,分别用直接干燥法、灼烧重量法、半微量凯氏定氮法、索氏抽提法对其水分、灰分、总蛋白、脂肪等营养指标进行测定,还原糖和总糖百分比通过DNS比色法测定,褐变度和ACE抑制活性分别采用分光光度法和HPLC法测定.结果显示,样品Y2的ACE抑制率和脂肪百分比均最高,分别为83.74%,12.92%;Y1中蛋白质百分比和褐变程度最高,其余指标为Y3最高,说明不同厂家生产的豆豉产品,其营养、感官及功能活性等指标均存在一定差异,工艺过程对豆豉相关品质的影响需要进一步研究.     

PTC124 targets genetic disorders caused by nonsense mutations

Nonsense mutations promote premature translational termination and cause anywhere from 5-70% of the individual cases of most inherited diseases. Studies on nonsense-mediated cystic fibrosis have indicated that boosting specific protein synthesis from <1% to as little as 5% of normal levels may greatly reduce the severity or eliminate the principal manifestations of disease. To address the need for a drug capable of suppressing premature termination, we identified PTC124-a new chemical entity that selectively induces ribosomal readthrough of premature but not normal termination codons. PTC124 activity, optimized using nonsense-containing reporters, promoted dystrophin production in primary muscle cells from humans and mdx mice expressing dystrophin nonsense alleles, and rescued striated muscle function in mdx mice within 2-8 weeks of drug exposure. PTC124 was well tolerated in animals at plasma exposures substantially in excess of those required for nonsense suppression. The selectivity of PTC124 for premature termination codons, its well characterized activity profile, oral bioavailability and pharmacological properties indicate that this drug may have broad clinical potential for the treatment of a large group of genetic disorders with limited or no therapeutic options.     

14-3-3sigma controls mitotic translation to facilitate cytokinesis

14-3-3 proteins are crucial in a wide variety of cellular responses including cell cycle progression, DNA damage checkpoints and apoptosis. One particular 14-3-3 isoform, sigma, is a p53-responsive gene, the function of which is frequently lost in human tumours, including breast and prostate cancers as a result of either hypermethylation of the 14-3-3sigma promoter or induction of an oestrogen-responsive ubiquitin ligase that specifically targets 14-3-3sigma for proteasomal degradation. Loss of 14-3-3sigma protein occurs not only within the tumours themselves but also in the surrounding pre-dysplastic tissue (so-called field cancerization), indicating that 14-3-3sigma might have an important tumour suppressor function that becomes lost early in the process of tumour evolution. The molecular basis for the tumour suppressor function of 14-3-3sigma is unknown. Here we report a previously unknown function for 14-3-3sigma as a regulator of mitotic translation through its direct mitosis-specific binding to a variety of translation/initiation factors, including eukaryotic initiation factor 4B in a stoichiometric manner. Cells lacking 14-3-3sigma, in marked contrast to normal cells, cannot suppress cap-dependent translation and do not stimulate cap-independent translation during and immediately after mitosis. This defective switch in the mechanism of translation results in reduced mitotic-specific expression of the endogenous internal ribosomal entry site (IRES)-dependent form of the cyclin-dependent kinase Cdk11 (p58 PITSLRE), leading to impaired cytokinesis, loss of Polo-like kinase-1 at the midbody, and the accumulation of binucleate cells. The aberrant mitotic phenotype of 14-3-3sigma-depleted cells can be rescued by forced expression of p58 PITSLRE or by extinguishing cap-dependent translation and increasing cap-independent translation during mitosis by using rapamycin. Our findings show how aberrant mitotic translation in the absence of 14-3-3sigma impairs mitotic exit to generate binucleate cells and provides a potential explanation of how 14-3-3sigma-deficient cells may progress on the path to aneuploidy and tumorigenesis.     

论食品安全学的理论基础与技术体系

食品安全学（foodsafetiology）是研究食物对人体健康危害的风险和保障食物无危害风险的学问,是食 品科学的一个分支,也是近３０年来发展起来的一门新兴学科。讨论、交流和理解食品安全学的理论基础与技术 体系将有助于促进食品安全学的科学研究、学科建设和人才培养,有助于加强国家食品安全管理和监管能力建 设。在研究大量有关国际组织文件、学术报告、会议文集,以及作者亲自考察、学术研究、自身理解的基础上, 提出了食品安全学的概念,归纳了食品安全学的理论基础和技术体系,探讨了食品安全学的学科构架。     

耐有机溶剂脂肪酶产生菌的筛选和鉴定

从国内各地采取土样分离筛选产耐有机溶剂脂肪酶的新菌株,通过形态和生理生化特征以及系统进化树分析,筛选到的新菌株命名为Pseudomonas aeruginosa CS-2.来自Pseudomonas aeruginosa CS-2的粗脂肪酶液在乙腈中酶活提高,在苯、氯仿、正己烷、石油醚和异辛烷中表现出较高的稳定性.     

临南洼陷油气藏分布特征与油气富集主控因素分析

临南洼陷具有良好的油气资源潜力,但随着油气勘探的逐渐深入,临南洼陷的勘探难度越来越大.为了更好的指导今后的油气勘探工作,在研究临南洼陷油气藏基本特征的基础上,从烃源岩、储集层、构造特征等多方面入手,总结油气藏分布特征和规律,分析油气富集成藏的主控因素,剖析各要素对油气富集的影响.结果表明,临南洼陷油气藏类型丰富,主要为断块油气藏及与断层有关的复合油气藏,油气藏主要分布在馆陶组、沙二段和沙三段.临南洼陷油气藏平面上主要分布在临商断裂、夏口断裂及其伴生构造带上,呈环状分布;浊积扇、冲积扇及河流沉积是油气藏分布的主要沉积类型;其富集主要受烃源岩、沉积相、断层等因素控制.油气藏受烃源岩控制围绕生油洼陷展布;有利沉积相带在平面上连续展布,纵向上叠置连片,具有良好的储集条件;断层作为油气的运移通道控制油气的运移,并与地层产状、构造等相配合形成圈闭,控制油气的聚集.     

基于软迭代的叠加训练序列信道估计方法

提出了一种基于软迭代的叠加训练序列信道估计方法.该方法将周期性训练序列与用户信息序列叠加后一起发送,通过软迭代方法,在接收端利用信道译码器输出的软信息来计算信息序列的一阶统计量,并将其反馈给信道估计器,用于消除观测量中来自未知信息的干扰,因此提高了信道的利用率和信道参数的估计精度.仿真结果表明,该方法有效地改善了信道估计性能,降低了接收机的误码率,提高了功率效率.