Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

SARS研究进展

本文对SARS病原学、流行病学、预防和治疗的研究进展进行了综述。     

设计式鉴定未知物实验教学初探

传统有机物性质实验教学体系和内容费时而意义不大,偏离了有机实验课教学主要目的和任务,不符合时代要求,改革势在必行.该文作者根据在理论上、实践上对传统有机物性质实验教学体系和内容进行的分析对其进行了重大改革,尝试新方法,提出了设计式鉴定未知物实验.实验学时从原来占40%减少到12%,大大提高了实验课效率,学生实验的主动性、运用知识分析问题解决问题能力和综合创新能力明显提高.     

中外低保管理面面观

目前我国城市居民最低生活保障制度的执行情况进展良好。低保政策的执行在保障下岗和失业人员基本生活及促进就业和再就业等方面发挥了明显的作用。但贫困问题是一个世界性问题 ,我们应借鉴各国在缓解贫困方面的作法 ,促进我国城市扶贫工作的顺利进行     

平顶山矿区塌陷地利用与可持续发展

矿区塌陷地是一种重要的土地资源,优化配置,合理利用,充分实现其资源价值为城市可持续发展提供资源保障。     

利雅得两性风景线(下)

一夫多妻制是真的吗?宗教警察可以掀开人们的盖头,看他有没有洗头。快乐在这里有着强烈的张力。     

发展中的苏州民营科技型企业

民营科技型企业，是在我国科技体制和经济体制改革的风暴之中诞生的产物，它既包括以科技人员为主体创办的，实行集体经济、合作经济、股份制经济和个体经济、私营经济的民办科技机构；又包括由国有科研院所、大专院校、大中型企业创办的，实行国有民营的科技型企业。     

农田变量作业机械导航系统

一、引言 20世纪90年代出现的精准农业技术是一种关于农业管理系统的战略思想,是信息科学技术和计算机技术在农业中的应用典范.精准农业技术是21世纪农业的新模式,是农业发展的必然趋势,对合理利用和保护农业资源、保护环境、降低成本、提高产品质量、减轻劳动强度、提高工作效率、增加经济效益等均起到积极的推进作用.     

GSM移动通信系统中语音隐藏算法研究

提出了一种能够在GSM移动通信网络中使用的信息隐藏算法。隐藏容量为50bit/s。实验结果表明算法对GSM、ADPCM等语音压缩编码以及滤波操作有很强的鲁棒性，携密语音的分段平均信噪比接近35dB，可以达到透明性要求。算法简单易行并且是基于盲检测的，具有很大的实用性。     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.