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In this article,current application,materials,key equipments,finite element(FE) simulation and parts properties of hot stamping are introduced.The investigations of all processes and further excellent processes are described.The survey of existing works,especially key equipments has revealed several gaps.Some new ideas and programs are proposed on the basis of traditional process.This article aims at providing an insight into a whole process backgrounds and pointing out the great potential for further investigations and innovations of hot stamping.  相似文献   
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Polyethyleneimine (PEI) is a well known gene transfection reagent that has been extensively studied in the pass two decades. In this work, we have explored its physiochemical properties by examining its interfacial adsorption at the hydrophilic silicon oxide/water interface and its ability to immobilize bioactive DNA under physiological conditions. Its surface excess was found to show a gradual increase with pH between pH 3 and pH 10. At pH 7 the surface adsorbed amount increased with increasing salt concentration. The amount of DNA immobilized was found to be controlled by the charge ratio between PEI and DNA under these conditions. Thus, the amount of bioactive DNA immobilized can be tuned by adjusting the amount of PEI preadsorbed. This study provides useful information for fabrication of biosensors and gene chips via interfacial adsorption.  相似文献   
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复合式静电感应电极水雾喷头的研究   总被引:1,自引:0,他引:1  
研究设计了一种复合式静电感应电极水雾喷头,它具有结构简单,荷电效果好,安全可靠等特点,并且克服了由于雾滴水流造成裸露电极的短路现象,同时该结构还避免了荷电后的水雾对密闭电极的静电屏蔽效应。  相似文献   
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建立了一种德语语料词性标注方法以及基于词性标注的统计方法.初步实验证明了上述方法对德语语料标注和标注后的语料进行单词、词类、短语结构和句子的统计是正确和有效的.  相似文献   
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Magnetic cloud is an important interplanetary dis-turbance structure generated by the coronal mass ejections(CMEs), it has been widely investigated[1—5] since it wassuggested by Burlaga et al[1]. However, the boundary ofthe magnetic cloud has no objective definition yet. Manyobservational signatures have been used to identify thecloud boundary[6—13], as Burlaga[14] indicated, there is noconsistency among these various approaches. The problemof the BLs was not been solved because of its co…  相似文献   
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基于线粒体COI基因序列探讨长江华溪蟹的遗传分化   总被引:6,自引:0,他引:6  
测定了长江华溪蟹3个亚种,包括指名亚种,桐柏亚种和陕县亚种共39个样本的线粒体细胞色素氧化酶亚基I(COI)基因部分的序列.重建的系统发生结果显示,长江华溪蟹分化成2个分支,一支代表雄性第1腹肢末节呈平直型的指名亚种支;另一支则代表雄性第1腹肢末节弯向背内方的内弯型支,包括稍向内弯的桐柏亚种和明显内弯的陕县亚种在内.在内弯型支内,桐柏亚种和陕县亚种之间的遗传距离为0.018,是两大分支间的1/10.提示长江华溪蟹COI基因的遗传分化与其雄性第1腹肢末节弯曲程度的形态分化基本一致.  相似文献   
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Kinetic knowledge is of great importance in achieving good control of the pyrolysis and gasification process and optimising system design. An overall kinetic pyrolysis scheme is therefore addressed here. The ki-netic modelling incorporates the following basic steps: the degradation of the virgin biomass materials into pri-mary products ( tar, gas and semi-char), the decomposition of primary tar into secondary products and the continuous interaction between primary gas and char. The last step is disregarded completely by models in the literature. Analysis and comparison of predicted results from different kinetic schemes and experimental data on our fixed bed pyrolyser yielded very positive evidence to support our kinetic scheme.  相似文献   
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Objective:The aims of this research were to purify and identify the 130 kDa(CagA) protein of H.pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa(CagA) pro-tein and gastric diseases.Methods:The procedure for isolating the protein included 6 mol/L guanidine ex-tract,size exclusion chromatography and elusion from gel.Sera of 68 patients with gastric diseases(44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer) were obtained,and the serological response to CagA was studied by Westem-blot using the purified protein,Results;The pu-rified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera,43 sera could recognize the purified protein associated with chronic agastritis 47.7%(21/44),atrophic gastritis 86.7%(13/15),peptic ulcer disease 100%(7/7),gastric cancer 100%(2/2).Compared with each other,the difference was significant(x^2=13.327,P=0.004),and 130 kDa(CagA) protein was associated with severe gastric diseases(rs=0.442,P=0.001).Conclusion:The 130 kDa(CagA) protein was associated wih severe gastric diseases.  相似文献   
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