Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya

Primary cilia are microtubule-based organelles that project from the surface of nearly every animal cell. Although important functions of primary cilia in morphogenesis and tissue homeostasis have been identified, the mechanisms that control the formation of primary cilia are not understood. Here we characterize a zebrafish gene, termed duboraya (dub), that is essential for ciliogenesis. Knockdown of dub in zebrafish embryos results in both defects in primary cilia formation in Kupffer's vesicle and randomization of left-right organ asymmetries. We show that, at the molecular level, the function of dub in ciliogenesis is regulated by phosphorylation, which in turn depends on Frizzled-2-mediated noncanonical Wnt signaling. We also provide evidence that, at the cellular level, dub function is essential for actin organization in the cells lining Kupffer's vesicle. Taken together, our findings identify a molecular factor that links noncanonical Wnt signaling with the control of left-right axis specification, and provide an entry point for analyzing the mechanisms that regulate primary cilia formation.     

CXorf6 is a causative gene for hypospadias

46,XY disorders of sex development (DSD) refer to a wide range of abnormal genitalia, including hypospadias, which affects approximately 0.5% of male newborns. We identified three different nonsense mutations of CXorf6 in individuals with hypospadias and found that its mouse homolog was specifically expressed in fetal Sertoli and Leydig cells around the critical period for sex development. These data imply that CXorf6 is a causative gene for hypospadias.     

新型城市共享单车智能借还系统的设计与实现

就目前而言,我国共享产品种类越发增多,共享单车作为最为常见且受欢迎的共享产品之一,于我国各个城市之中都得到了快速的普及推广。本文就新型城市共享单车智能借还系统的设计进行研究,旨在规范共享单车的借还操作,在方便单车借还的同时,有效延长单车的使用寿命。     

Deep levels,transport and THz emission properties of SiGe/Si quantum-well structures

In coal mines in such countries as China and Russia,most of the coal mine methane(CMM) generated during mining is emitted to the atmosphere without any effective usage,because the methane concentration of CMM is relatively low and not allowed to be used as fuel for safety reasons.Methane is one of the greenhouse gases.Therefore,if it becomes possible to concentrate CMM to an acceptable level for use as fuel,this will greatly contribute to reduction of greenhouse gas emissions.With the aim of gaining approva...     

和谐教学法在《机械制造工程》教学中的应用

《机械制造工程》是高校机械类专业中的一门主干专业基础课程,该课程对专业中的其他专业课程起着基础作用.基于当前部分高校的《机械制造工程》课堂气氛紧张、师生关系疏远、教学质量不高的现状,结合高校机械课堂教学实践,尝试在《机械制造工程》教学中运用和谐教学理念,探索一种随意并严谨、启发式的课堂互动,使课堂充满活泼的求知氛围,教学成为一种特殊的创新载体.     

VPN技术在企业网络中的应用

企业为了提升管理水平，加强信息沟通，提高运营效率，适应日益激烈的市场竞争和信息化发展，必须要建立一个高效稳定、覆盖面广、安全可靠的企业专用网络，使得企业内部、各子公司、分支机构及合作伙伴之间能实现信息的快速传递及数据的交换和共享。传统企业专用网络的组建一般是租用或自建专用传输线路，其建设及后期的维护、管理费用高昂。而VPN技术的出现，使得企业能根据自己的实际需求，以较低的成本和技术门槛，方便、快捷、灵活地组建属于自己的VPN虚拟专用网络。本文将对VPN技术的原理，其所具有的优势及在企业网络中的应用进行论述。     

枣南油田原油非牛顿渗流特性的实验研究

用实验方法研究了枣南孔一段、孔二段原油在油藏岩中渗流时、渗流速度、流度、视粘度及压力梯度等彼此间的变化关系。实验结果表明枣南油田地下原油具有非牛顿渗流特性，孔一段孔二段两种原油具有触变性拟塑型非牛顿流体性质。     

炸药柱热爆炸临界环境温度的研究

测定了改性１１０５（Ｇ１１０５）和聚奥１５９（ＪＯ１５９）两种塑料粘结炸药柱的３种尺寸（ｄ×ｌ分别为１０ｍｍ×２０ｍｍ，２０ｍｍ×３０ｍｍ，３０ｍｍ×４０ｍｍ）在非限定条件下的热爆炸临界环境温度；计算了在Ｔｈｏｍａｓ边界条件下（０〈Ｂｉ〈∞），３种长径比圆柱的热爆炸临界判据，结果表明，使用由ＤＳＣ分析出的热动力学数据可以很好地计算热稳定性较好的炸药柱的热爆炸临界环境温度。     

四甲基苯醌在氯化正丁基吡啶－AlCl＿3室温熔盐中的循环伏安行为

四甲基苯醌在氯化正丁基吡啶－ＡｌＣｌ＿３室温熔盐中的电化学行为，和室温熔盐的酸碱度有关，在碱性熔盐中，四甲基苯醌以不络合的形式存在，电极上的还原过程是一个ＥＣＥ过程；在酸性熔盐中，形成络合物ＴＱ（ＡｌＣｌ＿３）＿ｍ，络合数ｍ为１，２，３，随熔盐酸度变化，此外，醌类物质芳香环上的取代基，也影响其电化学性质。吸电子基团使还原电位正移，推电子基团使还原电位负移。