Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator （Alligator sinensis）

The Chinese alligator, Alligator sinensis, is a critically endangered species. A conservation project of gene resources for an endangered species first involves the preservation of organs, tissues, gametes, genomic DNA libraries and cell lines. The present study is the first to establish and cryopreserve cell lines of liver, heart and muscle tissues from the Chinese alligator. The study revealed that there was a large discrepancy in cell migration time in primary cultures among liver (11-12 d), heart (13-14 d) and muscle (17-18 d) tissue pieces. The differences in time in primary cell culture suggested that it was relatively easy to build visceral-derived cell lines for reptiles. Biological analysis showed that the population doubling time for thawed cells was approxi- mately 36 h. Karyotyping revealed that the frequency of Chinese alligator cells showing chromosome number as 2n=32 was 88.6%-93.4%. Chinese alligator cell lines established here provide a vital resource for research and are likely to be useful for protection of this rare and critically endangered species. Furthermore, the establishment of these methods may supply technical and theoretical support for preserving genetic resources at the cellular level for other reptile species.     

Experimental study on the explosive boiling in saturated liquid nitrogen

Nowadays,thedevelopmentandapplicationofsuperconductionisbecomingmoreandmoreimpor tantinmanyareas .Duringtheworkingprocessofsu perconductor,somefactors ,forinstance ,deforma tion ,friction ,andmagneticvortex ,canresultinin stantaneousandrandomheatimpulsion ,andmakeitover heatedtothecriticaltemperatureanddeviatefromthenormaloperationcondition .Undersomeex tremeconditions ,thesuperconductorcannotgobacktothenormalstateevenifremovingthetransientheat load ,thusverysevereandunexpectableconse quencema…     

Crystallization and preliminary X-ray diffraction analysis of FKBP12 complexed with a new neurotrophic ligand

Thetherapyofnerveinjuryandneurode generativediseasessuchasAlzheimer’sdiseaseandParkinson’sdiseaseisadifficultprojectforscientiststosurmount.Manydrugsandtherapeuticapproacheshavebeenusedtotreatthesediseases ,buttheresultisunsatisfactory[1] .Inrecent years ,neurotrophinsincludingnervegrowthfactor (NGF) ,neurotrophin3(NT3) ,neurotrophin 4 / 5 (NT4 / 5 )andbrain de rivedneurotrophicfactor (BDNF)werestudiedex tensivelyandfoundtoaffectthesurvival,growth ,and/ordifferentiationofneuronsandsever…     

Hausdorff centered measure of certain linear Cantor sets

Thenotionofpackingmeasure ,introducedinRefs.[1— 3],hasbeenusedincomparisonwithHausdorffmeasuretostudytheregularityandrectifi abilityofsetsintheplane[4 ] .TheHausdorffmeasureisdefinedusingeconomicalcoversofaset,whilethepackingmeasureisdefinedviapackingwithdisjointballscenteredinthegivenset (formoredetailsseeRefs .[5— 7]) .Asasymmetryofthepackingmea sure ,theHausdorffcenteredmeasure ,definedbythecoverswithballscenteredinthegivenset,wasintro ducedinRef .[4 ].RaymondandTricot[8] verifiedthatt…     

单圈连通图的边独立指数

依据图的边独立指数概念,探讨了单圈连通图的边独立指数的相关性质,得到了单圈连通图边独立指数的上、下界,并给出了单圈连通图边独立指数达到上、下界的极图     

基础教育改革下高校体育教育专业人才培养对策研究

通过选择部分高校的体育教育专业的教师和人才培养方案(教学计划)、部分中小学体育教师作为研究对象,运用文献资料法、问卷调查法、数理统计法等研究方法,对基础教育改革下的我国高校体育教育专业人才培养现状进行深入调查和客观分析,找出当前我国高校体育教育专业人才培养目标定位、培养内容,以及体育教育专业学生的教育思想、创新能力、综合素质不能很好地适应基础教育体育与健康课程改革的要求等现实问题,对高校体育专业人才培养的发展定位、目标体系、具体内容提出了相应的对策和建议     

183例小儿重度、特重度烧伤的临床治疗方法分析

目的研究小儿重度、特重度烧伤的临床救治.方法对我院2006年1月至2011年12月收治的183例重度、特重度小儿烧伤临床资料进行回顾总结及分析.结果183例患儿中治愈167例,治愈率为91.26%.结论早期及时有效的抗休克治疗是成功救治的重要保证,全身合理使用抗生素是防治烧伤感染的重要措施,积极预防烧伤并发症、维持气道通畅及合理创面外用药物、早期切削痂植皮封闭创面,是成功救治的重要环节     

中国股票市场的波函数

基于量子力学的基本假设,对 Zhang 和 Huang 提出的股票市场量子模型进行了改进,构建新的股票市场的哈密顿函数.通过求解相应的薛定谔方程,得到描述股票收益率的波函数.从理论上验证了在中国股票市场上,股票的价格受到市场信息的影响,因利好信息而升高,因不利信息而下降     

Research progress in synthesis and catalysis of polyoxometalates

Polyoxometalates (POMs) form a large class ofmetal oxide cluster type compounds. Their wealthytopology, physical and chemical properties have at tracted increasing interests. In this paper, based onour group’s research, we review the latest progress inthe synthetic chemistry and catalysis of POMs and in troduce some POMs with novel structure and theirderivatives that emerged in 2002—2004. Most of thecited references come from those published in 2003.Due to POMs’superio…     

Mutations analysis of STK_(11) gene in Chinese families with Peutz-Jeghers syndrome

Peutz-Jeghers syndrome (PJS) is an autosomal dominant disease that is characterized by multiple gastrointestinal hamartomatous polyps and melanin spots on lips and buccal mucosa at young age[1,2]. Previous studies have demonstrated that PJS predisposes carriers to cancers of gastrointestinal tract, uterus, ovary, testis, breast and other extragastrointestinal organs[3—5]. The STK11 gene, encoding a serine/threonine kinase at chro-mosome 19p13.3, was identified in 1998 as the main causativ…