Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator （Alligator sinensis）

The Chinese alligator, Alligator sinensis, is a critically endangered species. A conservation project of gene resources for an endangered species first involves the preservation of organs, tissues, gametes, genomic DNA libraries and cell lines. The present study is the first to establish and cryopreserve cell lines of liver, heart and muscle tissues from the Chinese alligator. The study revealed that there was a large discrepancy in cell migration time in primary cultures among liver (11-12 d), heart (13-14 d) and muscle (17-18 d) tissue pieces. The differences in time in primary cell culture suggested that it was relatively easy to build visceral-derived cell lines for reptiles. Biological analysis showed that the population doubling time for thawed cells was approxi- mately 36 h. Karyotyping revealed that the frequency of Chinese alligator cells showing chromosome number as 2n=32 was 88.6%-93.4%. Chinese alligator cell lines established here provide a vital resource for research and are likely to be useful for protection of this rare and critically endangered species. Furthermore, the establishment of these methods may supply technical and theoretical support for preserving genetic resources at the cellular level for other reptile species.     

无机抗菌剂的研究进展

本文介绍了无机抗菌剂的分类,通过对无机抗菌剂机理的介绍引出了对纳米抗菌剂的深刻探讨。最后,展望未来几年无机抗菌剂的发展方向。     

Experimental study on the explosive boiling in saturated liquid nitrogen

Nowadays,thedevelopmentandapplicationofsuperconductionisbecomingmoreandmoreimpor tantinmanyareas .Duringtheworkingprocessofsu perconductor,somefactors ,forinstance ,deforma tion ,friction ,andmagneticvortex ,canresultinin stantaneousandrandomheatimpulsion ,andmakeitover heatedtothecriticaltemperatureanddeviatefromthenormaloperationcondition .Undersomeex tremeconditions ,thesuperconductorcannotgobacktothenormalstateevenifremovingthetransientheat load ,thusverysevereandunexpectableconse quencema…     

Crystallization and preliminary X-ray diffraction analysis of FKBP12 complexed with a new neurotrophic ligand

Thetherapyofnerveinjuryandneurode generativediseasessuchasAlzheimer’sdiseaseandParkinson’sdiseaseisadifficultprojectforscientiststosurmount.Manydrugsandtherapeuticapproacheshavebeenusedtotreatthesediseases ,buttheresultisunsatisfactory[1] .Inrecent years ,neurotrophinsincludingnervegrowthfactor (NGF) ,neurotrophin3(NT3) ,neurotrophin 4 / 5 (NT4 / 5 )andbrain de rivedneurotrophicfactor (BDNF)werestudiedex tensivelyandfoundtoaffectthesurvival,growth ,and/ordifferentiationofneuronsandsever…     

Hausdorff centered measure of certain linear Cantor sets

Thenotionofpackingmeasure ,introducedinRefs.[1— 3],hasbeenusedincomparisonwithHausdorffmeasuretostudytheregularityandrectifi abilityofsetsintheplane[4 ] .TheHausdorffmeasureisdefinedusingeconomicalcoversofaset,whilethepackingmeasureisdefinedviapackingwithdisjointballscenteredinthegivenset (formoredetailsseeRefs .[5— 7]) .Asasymmetryofthepackingmea sure ,theHausdorffcenteredmeasure ,definedbythecoverswithballscenteredinthegivenset,wasintro ducedinRef .[4 ].RaymondandTricot[8] verifiedthatt…     

平面多层介质中空域Green函数的高效算法与应用

基于偶极子场理论，经过4个基本函数重构所有的矢量势和标量势谱域Green函数，并利用二级离散复镜像方法和高阶Sommerfeld恒等式，提出一种平面多层介质中的空域Green函数的高效算法．算法的优势在于：避免了利用传输线理论时需重写谱域Green函数各个分量，从而在不改变当前积分路径的前提下，直接提取表面波的影响，得到近区场和远区场都适用的空域Green函数，同时，有效地避免了电磁场混合势积分方程中电流（磁流）产生磁场（电场）的旋度因子，十分方便分析平面分层介质中三维结构的电磁散射和辐射特征，通过平面微带周期光子带隙（PBG）结构的S参数、大型微带天线阵列的雷达散射截面和平面分层介质中具有三维结构的目标的辐射场、散射场特性的数值分析，证明算法的有效性和正确性。     

单圈连通图的边独立指数

依据图的边独立指数概念,探讨了单圈连通图的边独立指数的相关性质,得到了单圈连通图边独立指数的上、下界,并给出了单圈连通图边独立指数达到上、下界的极图     

基础教育改革下高校体育教育专业人才培养对策研究

通过选择部分高校的体育教育专业的教师和人才培养方案(教学计划)、部分中小学体育教师作为研究对象,运用文献资料法、问卷调查法、数理统计法等研究方法,对基础教育改革下的我国高校体育教育专业人才培养现状进行深入调查和客观分析,找出当前我国高校体育教育专业人才培养目标定位、培养内容,以及体育教育专业学生的教育思想、创新能力、综合素质不能很好地适应基础教育体育与健康课程改革的要求等现实问题,对高校体育专业人才培养的发展定位、目标体系、具体内容提出了相应的对策和建议     

183例小儿重度、特重度烧伤的临床治疗方法分析

目的研究小儿重度、特重度烧伤的临床救治.方法对我院2006年1月至2011年12月收治的183例重度、特重度小儿烧伤临床资料进行回顾总结及分析.结果183例患儿中治愈167例,治愈率为91.26%.结论早期及时有效的抗休克治疗是成功救治的重要保证,全身合理使用抗生素是防治烧伤感染的重要措施,积极预防烧伤并发症、维持气道通畅及合理创面外用药物、早期切削痂植皮封闭创面,是成功救治的重要环节