钢筋混凝土牛腿抗剪承载力设计修正系数研究

为了研究钢筋混凝土牛腿的抗剪承载力,并评估我国和欧美规范牛腿设计方法的准确性和安全性,基于收集的209组牛腿竖向受剪试验数据对ACI 318-19、EC2、CSA A23.3-04和其他主要的牛腿抗剪承载力计算方法进行评估,发现欧美规范拉压杆模型方法较为保守,而软化拉压杆模型(softened strut-and-tie model,SSTM)能够较好地预测牛腿的抗剪承载力.在此基础上,设计了一系列满足我国规范要求的钢筋混凝土牛腿,主要变化的参数包括剪切跨度、混凝土强度、钢筋强度等,通过将牛腿的设计剪力与软化拉压杆模型计算得到的抗剪承载力进行对比,分析了不同设计参数下我国规范方法的准确性和安全性.并根据参数分析结果拟合了牛腿承载力修正系数的简化公式,并通过试验数据验证了简化公式的准确性和合理性.最后对牛腿在剪力作用下的设计方法提出了改进建议,可供钢筋混凝土牛腿设计参考.     

花生AhNCED1重组蛋白原核表达与二级结构初步分析

9-顺式环氧类胡萝卜素双加氧酶(NCED)是调控ABA生物合成的关键限速酶.根据花生叶片中克隆得到基因AhNCED1,构建融合蛋白重组表达载体,将重组质粒转化到大肠杆菌DH5a中诱导表达,SDS-PAGE分析显示在0.2 mmol/L IPTG、4 h、28 ℃的条件,目的蛋白以可溶形式高效表达,分子质量在66.0 ku左右.AhNCED1重组蛋白可与制备抗体特异性结合,呈现典型的不规则卷曲结构.     

食品中常见添加剂的测定与评价

随着社会主义经济体制的不断完善,人们物质生活水平越来越高,对食品的健康要求越来越严格。食品添加剂是提升食品色香味的重要成分,同时还可以起到防腐和提高食品品质的作用。然而近年来由于苏丹红,孔雀石绿,柠檬黄等食品添加剂带来的食品安全问题引起了社会各界的高度重视,在食品健康日益被关注的今天,添加剂的安全性能已经成为了时下的热点问题。本文结合个人多年实际工作经验,就食品中常见添加剂的测定和评价展开探讨,希望能够引起业内人士的广泛关注,起到抛砖引玉的作用。     

Sperm viability in the reproductive tract of females in a population of Sceloporus mucronatus exhibiting asynchronous reproduction

Asynchronous reproduction is a common phenomenon in high-elevation populations of lizards from Central México. Sperm storage in the reproductive tract of females is the mechanism for making oocyte fertilization possible. Our study addresses questions related to functional oviductal sperm storage of females mating on different dates throughout the reproductive season. A population of Sceloporus mucronatus with copulation in the summer and ovulation in the fall was chosen for this experiment. Eleven females that copulated in the field during June and 13 females that copulated in captivity during August were maintained in the laboratory until parturition. The number of pregnant females and the litter sizes produced in each experimental group were indicative of the viability of the stored sperm. Sperm stored in the reproductive tract of females were able to fertilize eggs after 4 months. No significant differences were found in the number of pregnant females between the 2 experimental groups nor in the litter sizes that they produced. We found that the amount of time sperm were held in the female reproductive tract (ca. 3 months) had no effect on the capacity of sperm to fertilize eggs. Histological examination of 8 oviducts collected before the mating season eliminated the possibility of sperm storage from one year to the next. In this system, sperm retention could have evolved as a response mechanism to deal with the asynchrony between sexes in the reproductive cycles. However, we cannot rule out alternative hypotheses.     

中原油田洒落原油对地下水污染的研究

洒落石油对地下水的污染主要分布在井下作业频繁的采油井井场内。在原油污染中心地带，表层土壤受污染最重，边缘地带稍轻；原油组分中非饱和烃运移能力最强；在天然条件下，原油污染物自然降解率较小；在污染区内，包气带中油污染向下运移并不与表层油污染浓度成正比。油污染在包气带中迁移受油污染浓度大小的控制，当石油污染物的浓度大于近似残余饱和度，污染物向下迁移，对地下水产生污染。     

钢铁企业产成品物流控制中转库流向的优化问题

以我国大型钢铁企业上海宝山钢铁集团(公司)为背景,描述了其产成品物流控制中的产成品转库问题,建立了转库流向优化问题的数学规划模型·使用遗传算法,提出一种称为“两段式可重复非负整数编码”的编码策略来获得大规模01规划模型的近优解·计算结果表明,当可用能力足够大并且准发数较少时,结果与SAS/OR的计算结果非常接近·当可用能力不是足够大,并且准发数较多时,计算结果要好于实际调度结果,此时SAS/OR软件在规定的时间内无法算出优化解·     

Simulating FAS-induced apoptosis by using P systems

In contrast to differential equations, P systems are an unconventional model of computation which takes into consideration the discrete character of the quantity of components and the inherent randomness that exists in biological phenomena. The key feature of P systems is their compartmentalised structure which represents the heterogeneity of the structural organisation of the cells, and where one can take into account the role played by membranes in the functioning of the system, for example signalling at the cell surface, selective uptake of substances from the media, diffusion across different compartments, etc. We show here that P systems can be a reliable tool for Systems Biology and could even outperform in some cases the current simulation techniques based on differential equations. We will also use a strategy based on the well known Gillespie algorithm but running on more than one compartment called Multi-compartmental Gillespie Algorithm.     

Genetic background of phenotypic variation

A noteworthy feature of the living world is its bewildering variability. A key issue in several biological disciplines is the achievement of an understanding of the hereditary basis of this variability. Two opposing, but not necessarily irreconcilable conceptions attempt to explain the underlying mechanism. The gene function paradigm postulates that phenotypic variance is generated by the polymorphism in the coding sequences of genes. However, comparisons of a great number of homologous gene and protein sequences have revealed that they predominantly remained functionally conserved even across distantly related phylogenic taxa. Alternatively, the gene regulation paradigm assumes that differences in the cis-regulatory region of genes do account for phenotype variation within species. An extension of this latter concept is that phenotypic variability is generated by the polymorphism in the overall gene expression profiles of gene networks. In other words, the activity of a particular gene is a system property determined both by the cis-regulatory sequences of the given genes and by the other genes of a gene network, whose expressions vary among individuals, too. Novel proponents of gene function paradigm claim that functional genetic variance within the coding sequences of regulatory genes is critical for the generation of morphological polymorphism. Note, however, that these developmental genes play direct regulatory roles in the control of gene expression.     

┱?例杨金彪;福建省南平市第

鸨?114尼美舒利致肝功能损伤1例陈瑶;李岚;厦门市妇幼保健院药剂科,上海儿童医学中心药剂科 厦门361000,上海200000尼美舒利;;肝功能损害0海峡药学Strait Pharmaceutical Journal118-119R725.9E06938E;E069_38陈瑶;114-115门诊药房实行专科药师制度的必要性陈成辉;何丽芳;     

Self-Dual Hadamard Bent Sequences

A new notion of bent sequence related to Hadamard matrices was introduced recently,motivated by a security application(Solé, et al., 2021). The authors study the self-dual class in length at most 196. The authors use three competing methods of generation: Exhaustion, Linear Algebra and Gr?bner bases. Regular Hadamard matrices and Bush-type Hadamard matrices provide many examples. The authors conjecture that if v is an even perfect square, a self-dual bent sequence of length v always exists. The ...