Mapping the Nuclear Fertility Restorer Gene in Rice with Simple Sequence Length Polymorphism （SSLP）

Bulked segregant analysis (BSA) of a F2 population derived from D62A/Ruby B was used to map the nuclear fertility restorer gene for wild abortive (WA) cytoplasmic male sterility. Three hundred and ninety-seven microsatellite primer pairs which distributed on 12 chromosomes were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants. One microsatellite marker RM182 located on chromosome 7 produced polymorphic products. The nuclear fertility restorer gene for WA cytoplasmic male sterility was mapped on chromosome 7, 7.4cM from RM182.     

α-amanitin线性肽骨架融合基因表达条件的优化

利用加端PCR技术在pGEX—1λT质粒BamHⅠ和pstⅠ位点间(片段BamHⅠ端)加入α-amanitin线性八肽碳骨架编码序列，再将两次连续加端PCR产物连入BamHⅠ、PstⅠ双酶切的pGEX—1λT载体，得到含α-amanitin线性碳骨架编码序列的阳性重组子pGAT-1．序列测定和结果分析显示，构建的融合基因相位正确，阅读框通读．通过对融合基因在大肠杆菌中表达条件的优化，目的融合蛋白的表达量可提高33．5％，占总蛋白表达量的28．3％．     

运用科技手段 实现单片机技术在装置艺术中的应用

随着科学技术的不断发展,计算机的应用越来越广,它不仅在工业、农业、国防、科研、教育等领域得到了普遍应用,而且在艺术领域也日益显示出强大的生命力,在设计装置艺术作品的过程中亦体现了科学和艺术的结合.     

俄罗斯优质水稻种质资源耐盐性鉴定和耐盐指标的评价

土壤盐渍化是全球性的问题,世界五大洲及其大多岛屿的滨海地区和干旱、半干旱地带都有分布．据联合国教科文组织（UNESCO）和粮农组织（FAO）的不完全统计,全世界盐渍土壤的面积为9．54亿公顷,约占世界陆地面积的7．6％．在我国盐渍土约有0．2亿公顷,分布于全国许多地区．此外,随着全球气温升高,降雨量减少,加之不适当灌溉等因素,使土壤次生盐渍化日趋加重．盐渍化土壤严重抑制作物生长发育,造成大幅度减产．因此,土壤盐渍化已经严重影响了全球农业的可持续发展．     

Infection Mechanism of Neovossaia horrida in Male Sterile Lines of Rice （Oryza sativa L. ）

After male sterile rice plants are inoculated with secondary sporidia of Neovossia horrida in the field,the process of infection is comparatively studied in four male sterile rice lines by scanning electmnmi-croscope. The infection process of Neovossia horrida is similar in these four male sterile lines. The results show, that the hyphae of fungus penetrates directly to the stigma, and then to the ovary after inoculation for 8h. The initial teliospore is formed between the aleurone layer and seed coat after 7d and matured in 9d. Although fungus penetrates through stigma without pollination, teliospore occurs only in the healthy developing endosperma.     

计算机辅助教学中应注意的几个问题

计算机辅助教学(Computer Asisted Instruction)简称CAI，是将计算机作为教学媒体并借助于预先制作的教学软件来展开的教学活动过程，是一种先进的教学手段，为学生提供良好的学习环境，目前，已经受到社会各界的普遍的关注。多媒体课件也越来越多地受到教师的青睐，传统教学手段的局限性越来越明显。在教学中，广泛地运用现代化的多媒体教学手段，已成为教学改革的必由之路。     

NCD系统下的损失分布参数估计

对投保人使用的奖惩系统下的追奖,使部分事故不出现索赔而导致损失频率高于报告索赔频率,对于有比例免赔和保险限额的汽车第三者责任保险,本文用动态规划方法评价和估计最优自留额.