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Relationship Between Stimulated Ethylene Production and Alternative Respiration Pathway in “Royal Gala” Apple Fruit 总被引:1,自引:0,他引:1
Endogenous ethylene production and alternative oxidase (AOX) protein expression in “Royal Gala”apple fruits were investigated after treatments with cold (0℃ for 1 week) and heat (38℃ for 1 h). Amonoclonal antibody to the terminal oxidase of the alternative pathway from Sauromatum guttatum was used to identify the AOX protein in apple fruits. The molecular mass of AOX in “Royal Gala” apple fruits is approximately 38 kDa, similar to those reported in tobacco and tomato. The cold treatment depressed the release of endogenous ethylene production before the climacteric ethylene production and obviously induced the expression of AOX protein expression. The heat treatment had the opposite effects on the ethylene production and AOX protein expression. In addition, the climax of endogenous ethylene production preceded the maximum AOX expression after the cold temperature treatment. It is therefore proposed that in climacteric fruits the production of induced ethylene is not coordinated with the level of AOX protein. 相似文献
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研究胸腺细胞体外培养条件下的~3H-Tdr、~3H-Ura及~3H-Phe的掺入能力,并对胸腺组织超氧化物歧化酶(SOD)的活性进行了测定。与对照组相比,经褪黑激素(Melatonin)处理的动物,其~3H-Tdr、~3H-Phe的掺入量分别增加57.1%与42.8%,~3H-Ura的掺入量变化不明显(1.10~1.48×10~)-2)dpm/cell);SOD活性增加了12.5%;同时发现,实验组动物的胸腺-体重比提高了14.1%。结果表明,褪黑激素对延缓小鼠胸腺的随龄退化有一定的效果。 相似文献
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IntroductionReactive oxygen species(ROS) generation has beenfound to be an important event in apoptotic tumorcell death induced by various anti- cancer agentssuch as camptothecin,vinblastine,inostamycinand adrimycin[1] .On the other hand,ROSscavengers such as N- acetyl- L- cysteine(NAC) andglutathione(GSH) have been found to inhibitapoptosis induced by TGFβ- 1 ,TNF-α and growthfactor deprivation in IL- 3 - dependent murine pro- Blymphocyte,murine T cell hybridoma,humanovarian- carci… 相似文献
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