The medaka draft genome and insights into vertebrate genome evolution

Teleosts comprise more than half of all vertebrate species and have adapted to a variety of marine and freshwater habitats. Their genome evolution and diversification are important subjects for the understanding of vertebrate evolution. Although draft genome sequences of two pufferfishes have been published, analysis of more fish genomes is desirable. Here we report a high-quality draft genome sequence of a small egg-laying freshwater teleost, medaka (Oryzias latipes). Medaka is native to East Asia and an excellent model system for a wide range of biology, including ecotoxicology, carcinogenesis, sex determination and developmental genetics. In the assembled medaka genome (700 megabases), which is less than half of the zebrafish genome, we predicted 20,141 genes, including approximately 2,900 new genes, using 5'-end serial analysis of gene expression tag information. We found single nucleotide polymorphisms (SNPs) at an average rate of 3.42% between the two inbred strains derived from two regional populations; this is the highest SNP rate seen in any vertebrate species. Analyses based on the dense SNP information show a strict genetic separation of 4 million years (Myr) between the two populations, and suggest that differential selective pressures acted on specific gene categories. Four-way comparisons with the human, pufferfish (Tetraodon), zebrafish and medaka genomes revealed that eight major interchromosomal rearrangements took place in a remarkably short period of approximately 50 Myr after the whole-genome duplication event in the teleost ancestor and afterwards, intriguingly, the medaka genome preserved its ancestral karyotype for more than 300 Myr.     

Method to Realize TT&C of Spacecraft In-Orbit from Ground-Based to Space-Ground-Based Mode

Currently,TT&C of spacecraft is performed by a ground system．Because more and more spacecrafts are launched into orbits,it is difficult for the ground system to meet the TT&C requirements of the spacecrafts．To explore a new,effective and economical TT&C mode is absolutely necessary．A method is proposed to use SGBM mode in TT&C spacecrafts in low earth orbits,here．Firstly,the principle of the mode is discussed;secondly,the system to simulate the mode is designed;thirdly,the relative issues to use the mode are indicated;fourthly,the experimental results（simulation）to apply the mode to TT&C spacecrafts are analyzed;and finally dawn is the main conclusion of the method．     

Self reliance and Innovation of Qinshan Phase Ⅱ NPP Project

This article mainly describes the selfreliance and innovation of Qinshan nuclear power project of phase II,inbetween it contains new reactor core design, as well as related experimental and calculation analysis, especially for new reactor design produced fluidinduced vibration model test, theoretical analysis and testing inbuilt reactor; aiming at twoloop NSSS a series improvement made for safety systems and related safety analysis to enhance their reliability and redundancy; according to specialty of twoloop NSSS an optimization made for NPP parameters and design of related equipments, for the purpose to make the output of NPP maximal; design of main reactor building and TG building also improved according to characteristics of twoloop NSSS and site conditions. CRDM and refueling machine are researched and manufactured on base of selfreliance, their performance are better than design requirements, large portion of key equipments are localized through different way. In construction first time realized the integrated erection of containment dome. During the commissioning nonnuclear steam driving of TG set, as well as 500 kV high voltage rising using emergent diesel generator, etc. are carried out. In period of operation still continuous innovation and improvement are made, so that to keep the good record of operation.     

Genome sequence and analysis of the tuber crop potato

Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and is central to global food security. It is clonally propagated, highly heterozygous, autotetraploid, and suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone to sequence and assemble 86% of the 844-megabase genome. We predict 39,031 protein-coding genes and present evidence for at least two genome duplication events indicative of a palaeopolyploid origin. As the first genome sequence of an asterid, the potato genome reveals 2,642 genes specific to this large angiosperm clade. We also sequenced a heterozygous diploid clone and show that gene presence/absence variants and other potentially deleterious mutations occur frequently and are a likely cause of inbreeding depression. Gene family expansion, tissue-specific expression and recruitment of genes to new pathways contributed to the evolution of tuber development. The potato genome sequence provides a platform for genetic improvement of this vital crop.     

Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome

Light is a fundamental signal that regulates important physiological processes such as development and circadian rhythm in living organisms. Phytochromes form a major family of photoreceptors responsible for red light perception in plants, fungi and bacteria. They undergo reversible photoconversion between red-absorbing (Pr) and far-red-absorbing (Pfr) states, thereby ultimately converting a light signal into a distinct biological signal that mediates subsequent cellular responses. Several structures of microbial phytochromes have been determined in their dark-adapted Pr or Pfr states. However, the structural nature of initial photochemical events has not been characterized by crystallography. Here we report the crystal structures of three intermediates in the photoreaction of Pseudomonas aeruginosa bacteriophytochrome (PaBphP). We used cryotrapping crystallography to capture intermediates, and followed structural changes by scanning the temperature at which the photoreaction proceeded. Light-induced conformational changes in PaBphP originate in ring D of the biliverdin (BV) chromophore, and E-to-Z isomerization about the C(15) = C(16) double bond between rings C and D is the initial photochemical event. As the chromophore relaxes, the twist of the C(15) methine bridge about its two dihedral angles is reversed. Structural changes extend further to rings B and A, and to the surrounding protein regions. These data indicate that absorption of a photon by the Pfr state of PaBphP converts a light signal into a structural signal via twisting and untwisting of the methine bridges in the linear tetrapyrrole within the confined protein cavity.     

Structural insight into brassinosteroid perception by BRI1

Brassinosteroids are essential phytohormones that have crucial roles in plant growth and development. Perception of brassinosteroids requires an active complex of BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1-ASSOCIATED KINASE 1 (BAK1). Recognized by the extracellular leucine-rich repeat (LRR) domain of BRI1, brassinosteroids induce a phosphorylation-mediated cascade to regulate gene expression. Here we present the crystal structures of BRI1(LRR) in free and brassinolide-bound forms. BRI1(LRR) exists as a monomer in crystals and solution independent of brassinolide. It comprises a helical solenoid structure that accommodates a separate insertion domain at its concave surface. Sandwiched between them, brassinolide binds to a hydrophobicity-dominating surface groove on BRI1(LRR). Brassinolide recognition by BRI1(LRR) is through an induced-fit mechanism involving stabilization of two interdomain loops that creates a pronounced non-polar surface groove for the hormone binding. Together, our results define the molecular mechanisms by which BRI1 recognizes brassinosteroids and provide insight into brassinosteroid-induced BRI1 activation.     

A novel process for comprehensive utilization of vanadium slag

Traditional processes for treating vanadium slag generate a huge volume of solid residue and a large amount of harmful gas, which cause serious environmental problems. In this study, a new process for the comprehensive utilization of vanadium slag was proposed, wherein zeolite A and a V₂O₅/TiO₂ system were synthesized. The structural properties of the as-synthesized zeolite A and the V₂O₅/TiO₂ system were characterized using various experimental techniques, including X-ray diffraction, X-ray fluorescence, scanning electron microscopy, and infrared spectroscopy. The results reveal that zeolite A and the V₂O₅/TiO₂ system are successfully obtained with high purity. The results of gas adsorption measurements indicate that the prepared zeolite A exhibits high selectivity for CO₂ over N₂ and is a candidate material for CO₂ capture from flue-gas streams.     

Taiga: Performance Optimization of the C4.5 Decision Tree Construction Algorithm

Classification is an important machine learning problem, and decision tree construction algorithms are an important class of solutions to this problem. Rain Forest is a scalable way to implement decision tree construction algorithms. It consists of several algorithms, of which the best one is a hybrid between a traditional recursive implementation and an iterative implementation which uses more memory but involves less write operations. We propose an optimized algorithm inspired by Rain Forest. By using a more sophisticated switching criterion between the two algorithms, we are able to get a performance gain even when all statistical information fits in memory. Evaluations show that our method can achieve a performance boost of 2.8 times in average than the traditional recursive implementation.     

随机变量列一类加权乘积和的强收敛性

讨论了任意r.v.列，两两NOD列和NA列的加权乘积和强收敛性，揭示了正则化因子，矩条件，权函数及r.v.列相关性之间的关系。     

Improved spectrum sharing algorithm based on feedback control information in cognitive radio networks

In order to avoid the system performance deterioration caused by the wireless fading channel and imperfect channel estimation in cognitive radio networks, the spectrum sharing problem with the consideration of feedback control information from the primary user is analyzed. An improved spectrum sharing algorithm based on the combination of the feedback control information and the optimization algorithm is proposed. The relaxation method is used to achieve the approximate spectrum sharing model, and the spectrum sharing strategy that satisfies the individual outage probability constraints can be obtained iteratively with the observed outage probability. Simulation results show that the proposed spectrum sharing algorithm can achieve the spectrum sharing strategy that satisfies the outage probability constraints and reduce the average outage probability without causing maximum transmission rate reduction of the secondary user.