A high-sensitive detection method for carvone odor by implanted electrodes in rat olfactory bulb

Mammalian olfactory systems have extraordi- nary ability to sense and identify various trace odorants. Taking advantages of cell culture and micro-fabrication technologies, olfactory cell- or tissue-based biosensor represent a promising platform for in vitro odorant detec- tion. However, in vitro conditions lead to shortened cell/ tissue survivals, and the working life of neuron chips is short. The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation. In this study, we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities. Then, we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains. The stable, repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks. Further, we explored the concentration detection sensitivity limitation of developed method, and found the detection low limit of carvone was below 10-a~ mol/L. The result demonstrates that the concentra- tion range of in vivo odorant detection method is much wider than in vitro method.     

EXISTENCE OF AUTHENTICATION CODES

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CSⅡ不育剂对光肩星天牛的小面积林间喷雾防治试验

在不育剂室内筛选试验已获成功的基础上，为弄清野外应用的效果，采用CSⅡ不育剂（可湿性粉剂）对光肩星天牛Anoplophora glabripennis(Motschulsky)进行了小面积林间喷雾防治试验。结果表明，采用此不育剂20倍液在天牛成虫羽化盛期进行林间喷雾可得到理想的不育效果，天牛卵及初孵幼虫的校正总不育率达72．08％。     

Multi-index analysis of the melting process of laterite metallized pellet

Herein, a multi-index analysis of the nickel content of an alloy, output rate of the alloy, nickel recovery rate, and iron recovery rate during the melting of laterite metallized pellets was performed. The thermodynamic reduction behavior of oxides such as NiO, FeO, Fe₃O₄, and Cr₂O₃ was studied using the FactSage software, which revealed that SiO₂ is not conducive to the reduction of iron oxides, whereas the addition of basic oxides such as CaO and MgO is beneficial for the reduction of iron oxides. On the basis of a comprehensive analysis to achieve greater nickel recovery and lower iron recovery rates, the optimum experimental parameters in the orthogonal experiment were A3B1C3 (t=30 min, C/O=0.4, R=1.2); the indicators w_Ni, φ_alloy, η_Ni, and η_Fe had values of 15.0wt%, 12.1%, 44.9%, and 96.4%, respectively. In single-factor experiments, increasing basicity (R) substantially improved the separation effect in the low-basicity range 0.5 ≤ R ≤ 0.8 but not in the high-basicity range 0.8 ≤ R ≤ 1.2. Similar results were obtained for the effect of the C/O ratio. Moreover, the recovery rate of nickel increased with increasing recovery rate of iron.     

Investigation on Coal-Oxygen Scrap Melting Process

Investigation on the coal-oxygen scrap melting process shows that using internal-combustion coal-oxygen burner to melt scrap is feasible. Among four types of coal-oxygen furnaces-oxygen burner melting furnace is better than other types on the quotas of process. The metal yield is 95%~97%, the melt down carbon and sulphur contents are >1.0% and <0.080% respectively for this melting furnace. The coal-oxygen scrap melting process would have a significant effect on the technological transformation of EAF and BOF in China.     

FICW： Frequent Itemset Based Text Clustering with Window Constraint

Most of the existing text clustering algorithms overlook the fact that one document is a word sequence with semantic information. There is some important semantic information existed in the positions of words in the sequence. In this paper, a novel method named Frequent Itemset-based Clustering with Window （FICW） was proposed, which makes use of the semantic information for text clustering with a window constraint. The experimental results obtained from tests on three （hypertext） text sets show that FICW outperforms the method compared in both clustering accuracy and efficiency.     

An all-silicon Raman laser

The possibility of light generation and/or amplification in silicon has attracted a great deal of attention for silicon-based optoelectronic applications owing to the potential for forming inexpensive, monolithic integrated optical components. Because of its indirect bandgap, bulk silicon shows very inefficient band-to-band radiative electron-hole recombination. Light emission in silicon has thus focused on the use of silicon engineered materials such as nanocrystals, Si/SiO2 superlattices, erbium-doped silicon-rich oxides, surface-textured bulk silicon and Si/SiGe quantum cascade structures. Stimulated Raman scattering (SRS) has recently been demonstrated as a mechanism to generate optical gain in planar silicon waveguide structures. In fact, net optical gain in the range 2-11 dB due to SRS has been reported in centimetre-sized silicon waveguides using pulsed pumping. Recently, a lasing experiment involving silicon as the gain medium by way of SRS was reported, where the ring laser cavity was formed by an 8-m-long optical fibre. Here we report the experimental demonstration of Raman lasing in a compact, all-silicon, waveguide cavity on a single silicon chip. This demonstration represents an important step towards producing practical continuous-wave optical amplifiers and lasers that could be integrated with other optoelectronic components onto CMOS-compatible silicon chips.     

Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac

The ability to taste the sweetness of carbohydrate-rich foodstuffs has a critical role in the nutritional status of humans. Although several components of bitter transduction pathways have been identified, the receptors and other sweet transduction elements remain unknown. The Sac locus in mouse, mapped to the distal end of chromosome 4 (refs. 7-9), is the major determinant of differences between sweet-sensitive and -insensitive strains of mice in their responsiveness to saccharin, sucrose and other sweeteners. To identify the human Sac locus, we searched for candidate genes within a region of approximately one million base pairs of the sequenced human genome syntenous to the region of Sac in mouse. From this search, we identified a likely candidate: T1R3, a previously unknown G protein-coupled receptor (GPCR) and the only GPCR in this region. Mouse Tas1r3 (encoding T1r3) maps to within 20,000 bp of the marker closest to Sac (ref. 9) and, like human TAS1R3, is expressed selectively in taste receptor cells. By comparing the sequence of Tas1r3 from several independently derived strains of mice, we identified a specific polymorphism that assorts between taster and non-taster strains. According to models of its structure, T1r3 from non-tasters is predicted to have an extra amino-terminal glycosylation site that, if used, would interfere with dimerization.     

Effects of C and Mn elements on deformation-enhanced ferrite transformation in low carbon (Mn) steels

Effects of C and Mn contents on the deformation-enhanced ferrite transformation (DEFT) in low carbon (Mn) steels have been investigated by hot compression. The microstructures of 2-4μm ultra-fine equiaxed ferrite grains with minors distributed homogeneously can be obtained by DEFT in all the tested steels. The more pronounced refinement is achieved as the C or Mn content increasing because of the higher-density nucleating sites and lower growth rate. The effectiveness of C on the level of refinement is more obvious than that of Mn.