SET domain protein lysine methyltransferases: Structure, specificity and catalysis

Site- and state-specific lysine methylation of histones is catalyzed by a family of proteins that contain the evolutionarily conserved SET domain and plays a fundamental role in epigenetic regulation of gene activation and silencing in all eukaryotes. The recently determined three-dimensional structures of the SET domains from chromosomal proteins reveal that the core SET domain structure contains a two-domain architecture, consisting of a conserved anti-parallel β-barrel and a structurally variable insert that surround a unusual knot-like structure that comprises the enzyme active site. These structures of the SET domains, either in the free state or when bound to cofactor S-adenosyl-L-homocysteine and/or histone peptide, mimicking an enzyme/cofactor/substrate complex, further yield the structural insights into the molecular basis of the substrate specificity, methylation multiplicity and the catalytic mechanism of histone lysine methylation. Received 10 June 2006; accepted 22 August 2006     

Cancer and blood coagulation

In human patients, blood coagulation disorders often associate with cancer, even in its early stages. Recently, in vitro and in vivo experimental models have shown that oncogene expression, or inactivation of tumour suppressor genes, upregulate genes that control blood coagulation. These studies suggest that activation of blood clotting, leading to peritumoral fibrin deposition, is instrumental in cancer development. Fibrin can indeed build up a provisional matrix, supporting the invasive growth of neoplastic tissues and blood vessels. Interference with blood coagulation can thus be considered as part of a multifaceted therapeutic approach to cancer. Received 30 November 2005; received after revision 7 February 2005; accepted 8 February 2006     

Functional interactions of protein kinase A and C in signalling networks: a recapitulation

On the basis of evidence collected from the literature, we propose a general model by which protein kinase (PK) A and the different PKC isoforms can inversely affect cell growth. Molecular switches, which are able to direct the signal towards antiproliferative or mitogenic pathways, are the different isoforms of Raf and PKC. Conflicting data are also reported and discussed in an attempt to reconcile them. Received 10 November 2005; received after revision 28 December 2005; accepted 3 January 2006     

喜马拉雅山中段达索普粒雪芯中夏季风和尘埃信号记录研究

通过粒雪芯δ＾１８Ｏ和主要离子（Ｃａ＾２＋,Ｍｇ＾２＋,ＮＨ＾＋４,ＳＯ＾２－４和ＮＯ＾－３）的分析表明,该粒雪芯年平均积累量为０．７５ｍ水当量。粒雪芯中δ＾１８Ｏ和主要离子浓度的季节变化表明,达索普冰川中高精度的记录了现代夏季风和尘埃信号。夏季风期间降水中δ＾１８Ｏ值由“降水量效应”所控制,雪层中较低δ＾１８Ｏ值则代表了夏季风信号。亚洲中部干旱半干旱区的春季尘暴输入是造成粒雪芯中Ｃａ＾２＋,Ｍｇ     

北极科学钻探计划

人们普遍认为,全球大洋水循环和气候演化同北极水域的水文地质和大气圈作用过程有关。对格陵兰岛冰心的最新研究表明,在最近的一个问冰期,仅仅由于格陵兰岛范围内冰的融化,就使洋而升高了6米。鉴于北极地质调查对于了解新生代时期地球大洋史和气候史的重要意义,现已制定了国际北极科学钻探计划（NADP）。俄罗斯、挪威、丹麦、英国、荷兰、德国、加拿大、美国、日本等国的科学家将联合研究两个主要问题：北极水域岩石圈的演化和新生代古环境演化。北极水域的岩石圈化研究表明,大约在1亿年前,由于北极微板块发生位移,北美板块在白垩…     

基于因子分析的新疆土地利用经济效益分析

运用经济学理论、因子分析方法,从选定的多个指标体系中提取少量具有代表性的指标,通过这些指标对新疆维吾尔自治区15个地州(市)土地利用经济效益状况进行分析;研究了各地州(市)土地利用的现状和特点,以及这些地区土地利用模式所带来的经济效益.从而探究新疆不同地区提高土地利用经济效益的对策,在此基础上提出了新疆提高土地利用经济效益的一些可行措施,以及进一步提高工业化程度、大力发展循环经济的对策.通过研究土地利用的经济效益,为确定合理的土地利用方式、结构及布局提供基础和依据,从而实现土地资源的可持续利用.充分体现出推动区域协调发展,优化国土开发格局;缩小区域发展差距;严格保护耕地,增加农业投入,促进农业科技进步,增强农业综合生产能力.图1,表4,参24.     

BEM Analysis of Wave Propagation in a Water-Filled Borehole in an Anisotropic Solid

This paper describes a time-domain boundary element method developed to analyze the interactions of acoustic and elastic waves near the interfaces between water and an anisotropic elastic solid. Two models are analyzed with one being the interface between two half spaces of fluid and solid and the other being a fluid region sandwiched by half space domains of anisotropic elastic solids. Both monopole and dipole point sources are used to generate an initial pressure wave in the fluid. Some snapshots of the transient wave behavior near the fluid-solid interfaces are given. The effect of the anisotropy in the solid on the pressure waveforms in the fluid is discussed. The numerical results allow detailed arrival identification and interpretation of acoustic and elastic waves propagating along the fluid-solid interfaces.     

蒙药好必斯清糖片质量标准的研究

"好必斯清糖片"是根据传统蒙医验方研制出的一种蒙药新型片剂.研究该药的质量标准,采用薄层色谱法(TLC)对其中的蒲黄、血竭、当归和益母草进行鉴别;用反相高效液相色谱法(HPLC)测定蒲黄中异鼠李素-3-O-新橙皮糖苷的含量.结果显示TLC色谱中能很好的检出蒲黄、血竭、当归和益母草的相应的化学成分;HPLC测定中异鼠李素-3-O-新橙皮糖苷含量在0.1-0.5μg范围内呈线性关系,回收率为98%(RSD=1.12%).这说明此方法灵敏,稳定,可以作为蒙药好必斯清糖片质量检测方法.     

基于EXCEL和MATLAB的矩形薄片热传导计算与仿真研究

对二维导热问题进行了离散化研究,推导出了节点差分方程,以矩形薄片为例,用MS.Excel对节点温度进行了计算,用MATLAB对计算结果进行了仿真,得到了薄片的温度分布仿真图.     

互联网大数据技术在智慧交通发展中的应用

随着互联网大数据技术的发展,各种互联网+交通的新业态不断涌现,行业内出现了大量丰富的数据资源。在交通强国建设背景下,更好地利用互联网大数据技术为智慧交通发展赋能成为重要任务。针对传统信号控制系统优化频率低,数据采集设备完备度差等缺陷,采用网约车轨迹大数据技术研究智慧信号控制优化系统,介绍了系统架构、特征和信号控制优化技术。该系统在济南、武汉和柳州等城市的实践结果表明,在不依赖渠化及路口改造的前提下,仅通过信号控制优化及软件升级的方式,能有效地降低重点路段和路口的工作日早晚高峰平均延误时间和停车次数等关键指标,其降幅可达10%～20%,能够有效缓解交通拥堵现状。