Preparation of luminescent CdTe quantum dots doped core-shell nanoparticles and their application in cell recognition

Quantum dots (QDs), often referring to “semiconductor nanocrystals”, have gained increasing attention in the past decade due to their unique optical properties compared with conventional organic fluorophore[1]. In the fields of biochemistry, cell biology and molecular biology, water- soluble QDs have played many important roles[2 - 8]. However, luminescent QDs are usually prepared in or-ganic solvents and the products are not water soluble, which make them unsuitable for biological applica…     

Effect of proton irradiation on structure relaxation of Zr<Subscript>41.5</Subscript>Ti<Subscript>14.9</Subscript>Cu<Subscript>12.6</Subscript>Ni<Subscript>10.5</Subscript>Be<Subscript>20.4</Subscript> bulk metallic glass

Effects of proton irradiation on structure re-laxation of Zr_(41.5)Ti_(14.9)Cu_(12.6)Ni_(10.5)Be_(20.4) bulk metallic glass areinvestigated by means of X-ray diffraction, differential scan-ning calorimetric and electronic resistance measurements.The results show that, at 203 K, the structure ofZr_(41.5)Ti_(14.9)Cu_(12.6)Ni_(10.5)Be_(20.4) bulk metal glass is relaxed obvi-ously by proton irradiation with the energy and the dosebeing of 160 keV and 1.65×10~(16) ions/cm~2, respectively. How-ever, Zr_(41 .5)Ti_(14.9)Cu_(12.6)Ni_(10.5)Be_(20.4) bulk metallic glass becomesmore steady amorphous state under two conditions, one isthat the energy and the dose are 160 keV and 1.1×10~(15)ions/cm~2, respectively. Another is that the energy and thedose are 120 keV and 6.5×10~(15) ions/cm~2, respectively.     

Expression and inhibitory activity analysis of a 25-kD Bowman-Birk protease inhibitor in rice

Bowman-Birk protease inhibitors (BBI), belonging to serine protease inhibitors, are well-studied storage and defense proteins widely existing in both dicoty- ledonous and monocotyledonous plants[1―3]. In dicoty- ledonous plants, the BBIs are about 8-kD p…     

In silico identification of silkworm selenoproteomes

Selenium (Se) is an essential trace element in vivo. Its biological function is mainly exerted through selenoproteins. Selenocysteine (Sec), the active site of selenoproteins, is incorporated into the protein at an in-frame TGA codon under the guidance of Sec insertion sequence (SECIS) element in the 3′-untranslated region (UTR) of the gene. In this work, a method was developed and a series of programs were edited by PERL language to in silico identify selenoproteomes from the genome of domesticated silkworm (Bombyx mori). Out of 18510 annotated genes, 6348 was terminated with TGA codons, 249 containing both in-frame TGAs and SECIS elements in the 3′-UTRs. Alignments of those selenoprotein candidates with their cysteine (Cys)-containing homologs revealed that 52 genes had TGA/Cys pairs and similar flanking regions around the in-frame TGAs. Restricted by the patterns of SECIS elements only 5 genes were screened out to fully meet the requirements for selenoproteins. Among them glutathione S-transferase (GST) has been reported as a microbial selenoprotein, the other four are novel selenoproteins annotated as CG6024, CG5195, ATP-binding cassette transporter subfamily A (ABCA), and nuclear VCP-like protein. Derived from the general properties of GST, ABCA and VCP, silkworm selenoproteins may play important roles in redox regulation, Se storage and transportation, as well as cell apoptosis.     

Secure Order-Specified Multisignature Scheme Based on DSA

0 IntroductionAmultisignature scheme is useful in the case thatplural signers generate a signaturefor a single mes-sage. Many multisignature schemes have been pro-posed[1-7]upto date.These schemes can be classifiedintotwo types by the basicintractable problemassumption aswell as single signature schemes ,i .e.discrete logarithmproblem(DLP) based schemes and RSA problem basedschemes .In an order-specified scheme the message and sig-ning order must be guaranteed. Mitomi and Miyaji[6]proposed…     

Identification and evolutionary implication of four novel box H/ACA snoRNAs from Giardia lamblia

During the biogenesis of eukaryotic ribosomes, the nascent pre-rRNA undergoes a complex pattern of nu- cleotide modifications of its mature rRNA sequences, including 2′-O-ribose methylation and pseudouridyla- tion, which are critical for the biogenesis a…     

Half-sandwich β-diketonato complexes of zirconium and their application as catalysts to ethylene polymerization

The bis(β-diketonato) Zr complexes CpZr(acac)2Cl (acac = acetylacetonato) (1) and Cp*Zr(acac)2Cl (2) can be synthesized by the reac-tion of Cp′ZrCl3(Cp′ = Cp or Cp*) with 2 equiv of lith-ium acetylacetonate. CpZr(acac)2Cl (1) can be quan-titatively prepared by treatment of Cp2ZrCl2 with ex-cess acetylacetone. The tris(β-diketonato) Zr com-plex CpZr(tfac)3 (tfac = trisfluoroacetylacetonato) (3) has been prepared by treatment of CpZrCl3 with so-dium trisfluoroacetylacetonate. The replacement of both chlorine atoms and one Cp group from zir-conocene dichloride by nonfluorinated diketone,acetylacetone,readily occurs at room temperature in triethylamine medium,offering the tris(β-diketonato) Zr complex CpZr(acac)3 (4). The complexes were characterized by 1H NMR spectroscopic methods. In addition,the molecular structures of complexes 3 and 4 have been determined by X-ray diffraction methods. When activated by excess methylaluminoxane (MAO),these complexes were active for ethylene polymerization,offering polyethylenes with high mo-lecular weights and narrow molecular weight distribu-tions. Complex 4 showed a very high ethylene po-lymerization activity of up to 7100 kg PE/mol Zr·h at 5 atm. Complexes 1 and 2 were also active for ethyl-ene/1-hexene copolymerization.     

Imaging mechanical shear waves induced by piezoelectric ceramics in magnetic resonance elastography

MRI is a noninvasive medical imaging modality for stimulating the development of medical diagnosis tech-nology. Scientists and engineers are now trying to extend the use of traditional MRI scanner to a wider field. The magnetic resonance elastography (MRE…     

Myotoxic activity of a Gln-49 phospholipase A2 from Agkistrodon blomhoffii ussurensis snake venom

A novel myotoxic protein phospholipase A₂ (PLA₂), denoted as Gln49-PLA₂, has been isolated from snake venom of Agkistrodon blomhoffii ussurensis, which has weak lethal effect and apparent anticoagulant activity, but lacks the PLA₂ and hemorrhagenic activity. Gln49-PLA₂ obviously increases of plasma creatine-kinase (CK) upon intramuscular injection in mice, suggesting that it may induce a dose-dependent myonecrosis. Histological studies also reveal morphological changes in mouse skeletal muscles, including extensive myonecrosis, hemorrhage and neutrophil infiltration in the treated animals. The myotoxic ability induced by Gln49-PLA₂ can be partially inhibited by heparin.