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51.
采用酶法和复合作用法对酵母菌株 13 0 0 (2N)和 2 0 10 (2N)的单倍体细胞 (1N)的获得率分别为3 0 %和 2 0 %左右 由实验确定脱子囊壁的最佳条件为采用生理盐水 ,pH5 .4 ,温度为 2 7℃ ,获得A型 ,B型两种单倍体细胞 (IN) 通过群体杂交法 ,从 75个平板上 ,挑选出 14 5个小菌落 ,得到 5个营养缺陷型菌株 ,该菌株的原生质体在 3 5 %PEG的作用下进行原生质体的融合 ,获得成功 但是仍需进一步对融合体进行生理、生态的研究 相似文献
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In this article,current application,materials,key equipments,finite element(FE) simulation and parts properties of hot stamping are introduced.The investigations of all processes and further excellent processes are described.The survey of existing works,especially key equipments has revealed several gaps.Some new ideas and programs are proposed on the basis of traditional process.This article aims at providing an insight into a whole process backgrounds and pointing out the great potential for further investigations and innovations of hot stamping. 相似文献
54.
Polyethyleneimine (PEI) is a well known gene transfection reagent that has been extensively studied in the pass two decades. In this work, we have explored its physiochemical properties by examining its interfacial adsorption at the hydrophilic silicon oxide/water interface and its ability to immobilize bioactive DNA under physiological conditions. Its surface excess was found to show a gradual increase with pH between pH 3 and pH 10. At pH 7 the surface adsorbed amount increased with increasing salt concentration. The amount of DNA immobilized was found to be controlled by the charge ratio between PEI and DNA under these conditions. Thus, the amount of bioactive DNA immobilized can be tuned by adjusting the amount of PEI preadsorbed. This study provides useful information for fabrication of biosensors and gene chips via interfacial adsorption. 相似文献
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复合式静电感应电极水雾喷头的研究 总被引:1,自引:0,他引:1
研究设计了一种复合式静电感应电极水雾喷头,它具有结构简单,荷电效果好,安全可靠等特点,并且克服了由于雾滴水流造成裸露电极的短路现象,同时该结构还避免了荷电后的水雾对密闭电极的静电屏蔽效应。 相似文献
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建立了一种德语语料词性标注方法以及基于词性标注的统计方法.初步实验证明了上述方法对德语语料标注和标注后的语料进行单词、词类、短语结构和句子的统计是正确和有效的. 相似文献
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WEI Fengsi FENG Xueshang YANG Fang & ZHONG Dingkun SIGMA Weather Group Laboratory for Space Weather Center for Space Science Applied Research Chinese Academy of Sciences Beijing China Correspondence should be addressed to Feng Xueshang 《科学通报(英文版)》2004,49(23):2533-2537
Magnetic cloud is an important interplanetary dis-turbance structure generated by the coronal mass ejections(CMEs), it has been widely investigated[1—5] since it wassuggested by Burlaga et al[1]. However, the boundary ofthe magnetic cloud has no objective definition yet. Manyobservational signatures have been used to identify thecloud boundary[6—13], as Burlaga[14] indicated, there is noconsistency among these various approaches. The problemof the BLs was not been solved because of its co… 相似文献
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Kinetic knowledge is of great importance in achieving good control of the pyrolysis and gasification process and optimising system design. An overall kinetic pyrolysis scheme is therefore addressed here. The ki-netic modelling incorporates the following basic steps: the degradation of the virgin biomass materials into pri-mary products ( tar, gas and semi-char), the decomposition of primary tar into secondary products and the continuous interaction between primary gas and char. The last step is disregarded completely by models in the literature. Analysis and comparison of predicted results from different kinetic schemes and experimental data on our fixed bed pyrolyser yielded very positive evidence to support our kinetic scheme. 相似文献
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Purification and relationship with gastric disease of a 130 kDa(CagA) protein of Helicobacter pylorl
Objective:The aims of this research were to purify and identify the 130 kDa(CagA) protein of H.pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa(CagA) pro-tein and gastric diseases.Methods:The procedure for isolating the protein included 6 mol/L guanidine ex-tract,size exclusion chromatography and elusion from gel.Sera of 68 patients with gastric diseases(44 with chronic gastritis,15 with atrophic gastritis,7 with peptic ulcer disease,2 with gastric cancer) were obtained,and the serological response to CagA was studied by Westem-blot using the purified protein,Results;The pu-rified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera,43 sera could recognize the purified protein associated with chronic agastritis 47.7%(21/44),atrophic gastritis 86.7%(13/15),peptic ulcer disease 100%(7/7),gastric cancer 100%(2/2).Compared with each other,the difference was significant(x^2=13.327,P=0.004),and 130 kDa(CagA) protein was associated with severe gastric diseases(rs=0.442,P=0.001).Conclusion:The 130 kDa(CagA) protein was associated wih severe gastric diseases. 相似文献