Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

科技期刊表格通用无线表和横线表的研究

提出科技期刊的表格,通用无线表和横线表的表式进行表达的理论和实践.论述了通用表表式的特点和要求,表头单、复式项目的设计,横视型和竖视型项目头的审改,不同栏数正、反线的合理应用,表身的恰当用线和疏密排.介绍了通用表的合并、转栏、分段、跨页的正确编排方法,以及特殊用表的变通方案.可供撰文、审校、编辑、排版工作借鉴.     

Characterization of the rough endoplasmic reticulum ribosome-binding activity.

The rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.     

公开密钥体制与Euler-Fermat定理通俗证明

随着计算机的广泛使用，文件加密解密已成为相当频繁的工作．本文叙述了公开密钥体制中的一种方法，并对其使用的数学工具给出一个通俗证明，以使加密解密工作更加方便、有效．     

浅议交流电动机调速时的温升变化

文章就交流电动机调速过程中电动机损耗变化作了分析,并讨论了温升所受的影响,详细推导了有关参数的计算方法。     

The Symbolic Meanings of the Forest in The Scarlet Letter by Nathaniel Hawthorn

The symbols in Hawthorne’s masterpiece The Scarlet Letter have the characteristics of indefiniteness and multi-sense. Each character brings out a different side of the forest,however the forest also brings out a different side in each character.The forest reflects multi-dimension. The image of ＂woods＂ or ＂forest＂ in The Scarlet Letter manifests Horthorne＇s understanding and exploring of the human nature. The forest may stand for individual freedom,innocence,truth,danger and evil as well.     

关于改进高职学生数学学习困难的探讨和研究

从学生的认知角度出发,对高职学生数学学习困难的认知特点及元认知特点进行了探讨,并提出了改进的方法和建议。