The mating behavior of individuals ofDrosophila pseudoobscura from New Zealand

Summary Recently discovered individuals in New Zealand of the North American speciesDrosophila pseudoobscura were analyzed for any divergence in mating behavior. Cultures collected from five localities in North American were mated with a stock from Rotorua, New Zealand. No significant divergence was obtained in any of the within North Americ, and more importantly, between North American and New Zealand mating experiments. Further analyses also showed no development of sterility between recently caught New Zealand and North American flies. We discuss our results in relation to others of this type.Publication No. 5 from the Evolutionary Genetics Laboratory, University of Auckland.Acknowledgements. We thank the University Grants Committee, grant No. 140Z88, for financial assistance. We thank D.G. Futch., L. Barr, A. Beckenach and H. Spencer for their kind assistance in sending us wild caught cultures.     

Murine arylsulfatase C: Evidence for two isozymes

Summary SWR/J mice posses high arylsulfatase C, estrone sulfatase, and dehydroepiandrosterone sulfatase activities in liver, spleen and kidney compared to A/J mice. This internstrain activity variation appears to be determined by at least 1 autosomal gene. Murine arylsulfatase C activity occurs in both hydrophobic and hydrophilic forms which differ with respect to certain biochemical properties and exhibit different subcellular distributions. The hydrophilic isozyme is a major component in kidney and brain extracts and a minor isozyme in liver and spleen extracts. The hydrophobic arylsulfatase C isozyme appears to be identical to steroid sulfatase. The hydrophilic arylsulfatase C isozyme does not possess steroid sulfatase activity; however, hydrophilic and hydrophobic arylsulfatase C share certain properties, suggesting that they may be structurally related. The autosomal gene(s) affects both arylsulfatase isozymes.This research was supported in part by National Institutes of Health grant GM 27707.     

Superoxide dismutase activity during the plasmodial life cycle ofPhysarum polycephalum

Summary Superoxide dismutase activity was slow throughout the cell cycle of surface cultures ofPhysarum polycephalum. This activity increased markedly when the organism was induced to spherulate. Glutathione (GSH) and hydrogen peroxide (H₂O₂) concentrations changed very little during the cell cycle. During spherulation GSH decreased; H₂O₂ and the cyanide-resistant respiration of plasmodial homogenates increased.     

Affinity chromatography of glucose dehydrogenase

Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies.     

Alteration in the SAT-chromosome ofAllium cepa affecting the size of the nucleolus

Resumen Se describe el hallazgo de un bulbo deAllium cepa en el cual los dos nucleolos de cada nÚcleo muestran una clara diferencia de tamaño. El nucleolo menor posee aproximadamente la mitad del diámetro del nucleolo normal. Esta alteración de la morfología nucleolar se correlaciona con la ausencia de satélite en uno de los dos cromosomas normalmente satelizados.

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The work reported in this paper was undertaken during the tenure of a Research Training Fellowship awarded by the International Agency for Research on Cancer.     

有机硫对维多利亚褐煤液化的催化作用之穆斯堡尔研究

我们以Morwell和Coolungoolun两种维多利亚褐煤进行实验,对铁和锡在煤的液化中的催化作用进行了比较,发现锡对低硫的Morwell煤较为有效,而铁则更适合于高硫的Coolungoolun煤。对液化余渣的穆斯堡尔分析的结果,使我们可以用低硫煤中元素态锡的形成以及在高硫煤中磁黄铁矿（Fe_(0.92)S）的形成来对观察到的现象作出解释。硫的损耗数据表明,磁黄铁矿的存在使得煤中的硫转化为气相的量增加了。     

Synthesis of rhodotorucine A,the inducing factor of mating tube formation ofRhodosporidium toruloides

Summary The inducing factor of mating tube formation ofRhodosporidium turuloides, named rhodotorucine A (H-Tyr-Pro-Glu-Ile-Ser-Trp-Thr-Arg-Asn-Gly-Cys(S-farnesyl)-OH), has been synthesized to confirm the structure proposed for the natural lipopeptide. The synthetic S-farnesyl undecapeptide has identical Rf values on TLC using several different solvents, and also the same biological activity as the natural hormone.Acknowledgments. We wish to express our thanks to Drs E. Ohmura, M. Nishikawa and M. Yoneda of Takeda Chemical Industries and Dr I. Banno of Institute for Fermentation, Osaka, for their encouragement throughout this work.