Geological record of fluid flow and seismogenesis along an erosive subducting plate boundary

Tectonic erosion of the overriding plate by the downgoing slab is believed to occur at half the Earth's subduction zones. In situ investigation of the geological processes at active erosive margins is extremely difficult owing to the deep marine environment and the net loss of forearc crust to deeper levels in the subduction zone. Until now, a fossil erosive subduction channel-the shear zone marking the plate boundary-has not been recognized in the field, so that seismic observations have provided the only information on plate boundary processes at erosive margins. Here we show that a fossil erosive margin is preserved in the Northern Apennines of Italy. It formed during the Tertiary transition from oceanic subduction to continental collision, and was preserved by the late deactivation and fossilization of the plate boundary. The outcropping erosive subduction channel is approximately 500 m thick. It is representative of the first 5 km of depth, with its deeper portions reaching approximately 150 degrees C. The fossil zone records several surprises. Two décollements were simultaneously active at the top and base of the subduction channel. Both deeper basal erosion and near-surface frontal erosion occurred. At shallow depths extension was a key deformation component within this erosive convergent plate boundary, and slip occurred without an observable fluid pressure cycle. At depths greater than about 3 km a fluid cycle is clearly shown by the development of veins and the alternation of fast (co-seismic) and slow (inter-seismic) slip. In the deepest portions of the outcropping subduction channel, extension is finally overprinted by compressional structures. In modern subduction zones the onset of seismic activity is believed to occur at approximately 150 degrees C, but in the fossil channel the onset occurred at cooler palaeo-temperatures.     

PML targeting eradicates quiescent leukaemia-initiating cells

The existence of a small population of 'cancer-initiating cells' responsible for tumour maintenance has been firmly demonstrated in leukaemia. This concept is currently being tested in solid tumours. Leukaemia-initiating cells, particularly those that are in a quiescent state, are thought to be resistant to chemotherapy and targeted therapies, resulting in disease relapse. Chronic myeloid leukaemia is a paradigmatic haematopoietic stem cell disease in which the leukaemia-initiating-cell pool is not eradicated by current therapy, leading to disease relapse on drug discontinuation. Here we define the critical role of the promyelocytic leukaemia protein (PML) tumour suppressor in haematopoietic stem cell maintenance, and present a new therapeutic approach for targeting quiescent leukaemia-initiating cells and possibly cancer-initiating cells by pharmacological inhibition of PML.     

The critical role of disks in the formation of high-mass stars

Although massive stars (commonly defined as those in excess of about eight solar masses, or with initial luminosities of a thousand times the solar luminosity or more) have an enormous impact on the galactic environment, how they form has been a mystery. The solution probably involves the existence of accretion disks. Rotational motions have been found in the gas surrounding young high-mass stars, which suggests that non-spherical accretion could be the fundamental ingredient of the massive-star formation recipe.     

Piola’s contribution to continuum mechanics

This paper examines the contribution of Gabrio Piola to continuum mechanics.Though he was undoubtably a skilled mathematician and a good mechanician, little is commonly known about his papers within the international scientific community, principally because a large part of the Italian school of mechanics was isolated in the first half of the XIXth century.We examine and comment on Piola’s most important papers, and compare them with those of his contemporaries Cauchy, Poisson and Kirchhoff.     

Thiamine and its mono-, di-, and triphosphoric esters content of normal rat tissues

Riassunto L'uso di un nuovo metodo cromatografico per la separazione della Tiamina e dei suoi esteri mono, di, e trifosforico nei tessuti animali ha permesso di definire, per la prima volta in termini quantitativi, la presenza del trifosfato nei tessuti stessi. L'organo più ricco di questo estere è il fegato, seguito dal cuore, dal rene e dal cervello nell'ordine.     

Phosphorylation of thiamine in the intestinal wall during absorption in vivo

Riassunto 2 h dopo l'introduzione di tiamina cloridrato o di tiaminpropildisolfuro nell'intestino tenue di ratto in vivo si trova un aumento significativo di tiamina fosforilata nella parete intestinale, essenzialmente a carico del tiamindifosfato.     

8-oxo-guanine bypass by human DNA polymerases in the presence of auxiliary proteins

Specialized DNA polymerases (DNA pols) are required for lesion bypass in human cells. Auxiliary factors have an important, but so far poorly understood, role. Here we analyse the effects of human proliferating cell nuclear antigen (PCNA) and replication protein A (RP-A) on six different human DNA pols--belonging to the B, Y and X classes--during in vitro bypass of different lesions. The mutagenic lesion 8-oxo-guanine (8-oxo-G) has high miscoding potential. A major and specific effect was found for 8-oxo-G bypass with DNA pols lambda and eta. PCNA and RP-A allowed correct incorporation of dCTP opposite a 8-oxo-G template 1,200-fold more efficiently than the incorrect dATP by DNA pol lambda, and 68-fold by DNA pol eta, respectively. Experiments with DNA-pol-lambda-null cell extracts suggested an important role for DNA pol lambda. On the other hand, DNA pol iota, together with DNA pols alpha, delta and beta, showed a much lower correct bypass efficiency. Our findings show the existence of an accurate mechanism to reduce the deleterious consequences of oxidative damage and, in addition, point to an important role for PCNA and RP-A in determining a functional hierarchy among different DNA pols in lesion bypass.