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71.
Cellular biochemical networks have to function in a noisy environment using imperfect components. In particular, networks involved in gene regulation or signal transduction allow only for small output tolerances, and the underlying network structures can be expected to have undergone evolution for inherent robustness against perturbations. Here we combine theoretical and experimental analyses to investigate an optimal design for the signalling network of bacterial chemotaxis, one of the most thoroughly studied signalling networks in biology. We experimentally determine the extent of intercellular variations in the expression levels of chemotaxis proteins and use computer simulations to quantify the robustness of several hypothetical chemotaxis pathway topologies to such gene expression noise. We demonstrate that among these topologies the experimentally established chemotaxis network of Escherichia coli has the smallest sufficiently robust network structure, allowing accurate chemotactic response for almost all individuals within a population. Our results suggest that this pathway has evolved to show an optimal chemotactic performance while minimizing the cost of resources associated with high levels of protein expression. Moreover, the underlying topological design principles compensating for intercellular variations seem to be highly conserved among bacterial chemosensory systems. 相似文献
72.
Hjorth J Watson D Fynbo JP Price PA Jensen BL Jørgensen UG Kubas D Gorosabel J Jakobsson P Sollerman J Pedersen K Kouveliotou C 《Nature》2005,437(7060):859-861
It has long been known that there are two classes of gamma-ray bursts (GRBs), mainly distinguished by their durations. The breakthrough in our understanding of long-duration GRBs (those lasting more than approximately 2 s), which ultimately linked them with energetic type Ic supernovae, came from the discovery of their long-lived X-ray and optical 'afterglows', when precise and rapid localizations of the sources could finally be obtained. X-ray localizations have recently become available for short (duration <2 s) GRBs, which have evaded optical detection for more than 30 years. Here we report the first discovery of transient optical emission (R-band magnitude approximately 23) associated with a short burst: GRB 050709. The optical afterglow was localized with subarcsecond accuracy, and lies in the outskirts of a blue dwarf galaxy. The optical and X-ray afterglow properties 34 h after the GRB are reminiscent of the afterglows of long GRBs, which are attributable to synchrotron emission from ultrarelativistic ejecta. We did not, however, detect a supernova, as found in most nearby long GRB afterglows, which suggests a different origin for the short GRBs. 相似文献
73.
C. A. Baile F. Anne Scott J. Mayer 《Cellular and molecular life sciences : CMLS》1967,23(12):1033-1034
Résumé Par des lésions bilatérales hypothalamiques on a rendu des rats aphagiques et adipsiques. Durant la période de convalescence, on a injecté dans le ventricule latéral du pentotal de sodium (ce qui normalement fait manger les animaux) et une solution saline hypertonique (qui normalement les fait boire). On a trouvé qu'après les lésions les rats ne réagissent que lorsqu'ils sont redevenus capables de manger et de boire spontanément.
This work was supported in part by a grant from the National Institute of Neurological Diseases and Blindness, No. NB 01941, of the National Institutes of Health, U.S. Public Health Service, Bethesda, Maryland; by a grant from the National Science Foundation, No. GB-4594, Washington, D.C.; and the Fund for Research and Teaching of the Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts. 相似文献
This work was supported in part by a grant from the National Institute of Neurological Diseases and Blindness, No. NB 01941, of the National Institutes of Health, U.S. Public Health Service, Bethesda, Maryland; by a grant from the National Science Foundation, No. GB-4594, Washington, D.C.; and the Fund for Research and Teaching of the Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts. 相似文献
74.
Two decades of scientific ocean drilling have demonstrated widespread microbial life in deep sub-seafloor sediment, and surprisingly high microbial-cell numbers. Despite the ubiquity of life in the deep biosphere, the large community sizes and the low energy fluxes in this vast buried ecosystem are not yet understood. It is not known whether organisms of the deep biosphere are specifically adapted to extremely low energy fluxes or whether most of the observed cells are in a dormant, spore-like state. Here we apply a new approach--the D:L-amino-acid model--to quantify the distributions and turnover times of living microbial biomass, endospores and microbial necromass, as well as to determine their role in the sub-seafloor carbon budget. The approach combines sensitive analyses of unique bacterial markers (muramic acid and D-amino acids) and the bacterial endospore marker, dipicolinic acid, with racemization dynamics of stereo-isomeric amino acids. Endospores are as abundant as vegetative cells and microbial activity is extremely low, leading to microbial biomass turnover times of hundreds to thousands of years. We infer from model calculations that biomass production is sustained by organic carbon deposited from the surface photosynthetic world millions of years ago and that microbial necromass is recycled over timescales of hundreds of thousands of years. 相似文献
75.
Chromatin-modifying enzymes as modulators of reprogramming 总被引:2,自引:0,他引:2
Onder TT Kara N Cherry A Sinha AU Zhu N Bernt KM Cahan P Marcarci BO Unternaehrer J Gupta PB Lander ES Armstrong SA Daley GQ 《Nature》2012,483(7391):598-602
76.
77.
The mechanisms by which mafic rocks become converted to denser eclogite in the lower crust and mantle are fundamental to our understanding of subduction, mountain building and the long-term geochemical evolution of Earth. Based on larger-than-expected gradients in argon isotopes, Camacho et al. propose a new explanation--co-seismic injection of hot (700 degrees C) aqueous fluids into much colder (400 degrees C) crust--for the localized nature of eclogite metamorphism during Caledonian crustal thickening, as recorded in the rocks of Holsn?y in the Bergen arcs, western Norway. We have studied these unusual rocks, which were thoroughly dehydrated under granulite facies conditions during a Neoproterozoic event (about 945 million years (945 Myr) ago); we also concluded that fracture-hosted fluids were essential as catalysts and components in the conversion to eclogite about 425 Myr ago. However, we are sceptical of the assertion by Camacho et al. that eclogite temperatures were reached only in the vicinity of fluid-filled fractures. Determining whether these rocks were strong enough to fracture at depths of 50 km because they were cold or because they were very dry is crucial to understanding the mechanics of the lower crust in mountain belts, including, for example, the causes of seismicity in the Indian plate beneath the modern Himalayas. 相似文献
78.
79.
Centrioles are necessary for flagella and cilia formation, cytokinesis, cell-cycle control and centrosome organization/spindle assembly. They duplicate once per cell cycle, but the mechanisms underlying their duplication remain unclear. Here we show using electron tomography of staged C. elegans one-cell embryos that daughter centriole assembly begins with the formation and elongation of a central tube followed by the peripheral assembly of nine singlet microtubules. Tube formation and elongation is dependent on the SAS-5 and SAS-6 proteins, whereas the assembly of singlet microtubules onto the central tube depends on SAS-4. We further show that centriole assembly is triggered by an upstream signal mediated by SPD-2 and ZYG-1. These results define a structural pathway for the assembly of a daughter centriole and should have general relevance for future studies on centriole assembly in other organisms. 相似文献
80.
Epigenetic concepts are fundamentally shaped by a legacy of negative definition, often understood by what they are not. Yet the function and implication of negative definition for scientific discourse has thus far received scant attention. Using the term epimutation as exemplar, we analyze the paradoxical like-but-unlike structure of a term that must simultaneously connect with but depart from genetic concepts. We assess the historical forces structuring the use of epimutation and like terms such as paramutation. This analysis highlights the positive characteristics defining epimutation: the regularity, oxymoronic temporality, and materiality of stable processes. Integrating historical work, ethnographic observation, and insights from philosophical practice-oriented conceptual analysis, we detail the distinctive epistemic goals the epimutation concept fulfils in medicine, plant biology and toxicology. Epimutation and allied epigenetic terms have succeeded by being mutation-like and recognizable, yet have failed to consolidate for exactly the same reason: they are tied simultaneously by likeness and opposition to nouns that describe things that are assumed to persist unchanged over space and time. Moreover, negative definition casts the genetic-epigenetic relationship as an either/or binary, overshadowing continuities and connections. This analysis is intended to assist practitioners and observers of genetics and epigenetics in recognizing and moving beyond the conceptual legacies of negative definition. 相似文献