Host cell reactivation of ozone-treated T3 bacteriophage by different strains ofEscherichia coli

Summary Host cell reactivation capacity for ozone T3 phage was determined for differentE. coli strains deficient in one or more of the DNA repair mechanisms. The results indicate that DNA polymerase I appears to play a key role in the repair of damage produced on the DNA by ozone while thelexA gene product seems to play a minor one.This research was sponsored by the National Sciences and Engineering Council of Canada. One of us (PLH) acknowledges a scholarship from the NSERCof Canada.     

伪随机序列的均匀性评价

伪随机序列生成方法有很多种 ,因生成方法不同而导致其性质的差异较大 .文中描述了产生均匀分布的U (0 ,1 )伪随机序列的数学原理 ,提出了评估均匀分布伪随机序列均匀性的几种方法 ,对由软件产生的几种均匀分布序列的均匀性作出了评估     

杨辉三角与行列式

本文用杨辉三角构造了两种类型的行列式，然后逐一讨论了这两类行列式的值，并由此得到了它们一些特例的简洁结果。     

动力学分析法测定银的进展

本文综述了动力学分析法测定银的发展概况.并对测定方法列表进行了比较,文章引用国内外文献90篇.     

Increased assembly of cytoskeletal proteins associated with the transformation of human liver cells into fibroblast-like cells.

A topoisomerase II inhibitor, novobiocin, and a deacetylase inhibitor, butyrate, synergistically transformed human liver cells into fibroblast-like cells. This morphological change was associated with an increased production of procollagen type III peptide and a simultaneous assembly of actin, tubulin, vimentin and cytokeratin. Novobiocin and butyrate had no marked effect on the phosphorylation state of cytokeratin proteins, but synergistically enhanced [3H]acetate uptake. From these results, it can be speculated that protein acetylation plays an important role in inducing the assembly of cytoskeletal proteins and the morphological transformation of human liver cells.     

三氮烯类化合物作为酸碱指示剂的研究及应用

本文报道了三氮烯类化合物作为酸碱指示剂的研究及应用.以我们新近合成的此类试剂之一——2-氯一4一硝基苯基重氮氨基偶氮苯为研究主要对象,分别讨论了有无表面活性剂存在、以及不同类型表面活性剂存在下,试剂的离解常数及变色区域.在TritonX-100存在下,其试剂的pKa=9.25±0.01,变色区域为pH8.40(黄)～10.0(紫);在CTMAB存在下,pKa=7.35士0.03,变色区域pH6.85～7.90;在混合表面活性剂TritonX-100-CTMAB存在下,pKa=8.20±0.05,变色域pH7.45～9.00(黄～紫).特别是在阳离子表面活性剂存在下,滴定终点颜色变化十分敏锐清晰,是一种优于酚酞和甲基橙的指示剂.最后并对此类试剂与表面活性剂的相互作用作了简单探讨.     

Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

水下沉桩施工新技术

肇庆换流站供水工程之一的五尺峡取水泵站施工中，在受施工条件限制的情况下，采用将钢筋混凝土预制桩改为开口钢管桩，将双排桩改为单排桩，尝试搭设水上竹平台作沉桩施工平台等措施均取得成功，保证了质量、安全、进度，降低了施工成本．     

食物安全的内涵及其与相关概念的比较

食物是人类赖以生存和发展的基本物质基础,食物安全状况不仅关系到人民的身体健康,更影响到国家的安全和民族的整体素质.因此,食物安全成为政府重视、社会关注和人民群众极大关心的焦点,也必然地成为科学研究的重点课题.     

双间隙加速管设计中几个问题的研究

对加速管的放电或击穿与绝缘环，电极材料，极间距离和真空度等因素的关系进行了分析，为加速管的设计提供了依据。以此研制的加速算运行正常。