Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel

The complete amino-acid sequence of the cyclic GMP-gated channel from bovine retinal rod photoreceptors, deduced by cloning and sequencing its complementary DNA, shows that the protein contains several putative transmembrane segments, followed by a region that is similar to the cyclic GMP-binding domains of cyclic GMP-dependent protein kinase. Expression of the complementary DNA produces cyclic GMP-gated channel activity in Xenopus oocytes.     

Mutation in codon 713 of the beta amyloid precursor protein gene presenting with schizophrenia.

Following reports of mutations of codon 717 in exon 17 of the amyloid precursor protein (APP) gene in early-onset familial Alzheimer's disease, we screened exon 17 for new mutations in presenile dementia. The majority of the 105 patients screened had definite or probable Alzheimer's disease, but we also included atypical cases and some chronic schizophrenics. We identified a single abnormal case--a chronic schizophrenic with cognitive defects. Sequencing revealed a C to T nucleotide substitution which produces an alanine to valine change at codon 713. We were unable to detect the mutation in the remaining members of the original cohort nor in a further 100 chronic schizophrenics and 100 non-demented controls. Nonetheless, the position of the mutation in a critical portion of the APP gene suggests that it may well prove to be pathogenic.     

The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus.

T-associated maternal effect (Tme) is the only known maternal-effect mutation in the mouse. The defect is nuclear-encoded and embryos that inherit a deletion of the Tme locus from their mother die at day 15 of gestation. There are many genomically imprinted regions known in the mouse genome but so far no imprinted genes have been cloned. The Tme locus is absent in two chromosome-17 deletion mutants, Thp and the tLub2, and its position has been localized using these deletions to a 1-cM region. We report here that the genes for insulin-like growth factor type-2 receptor (Igf2r) and mitochondrial superoxide dismutase-2 (Sod-2) are absent from both deletions. Probes for these genes and for plasminogen (Plg) and T-complex peptide 1 (Tcp-1) were used in pulsed-field gel mapping to show that Tme must lie within a region of 800-1,100 kb. We also demonstrate that embryos express Igf2r only from the maternal chromosome, and that Tcp-1, Plg and Sod-2 are expressed from both chromosomes. Therefore Igf2r is imprinted and closely linked or identical to Tme.     

Maximale tubuläre Zuckerresorption (Tmg) und Phosphorylierung

Summary Tmg (maximal tubular reabsorption of glucose) can be raised enormously by intravenous administration of Percorten. Therefore we suggest that the degree of reabsorption of glucose depends on the power of phosphorylation of the tubular tissue.     

Vasopressin analogues with selective pressor activity

Zusammenfassung Die Synthese und die pharmakologischen Haupteigenschaften von Orn⁸-Vasopressin, Phe²-Orn⁸-Vasopressin, Orn⁸-Oxytocin und Phe²-Orn⁸-Oxytocin werden beschrieben. Die pressorische Aktivität dieser Peptide zeigt eine in der Reihenfolge zunehmende Selektivität.     

The formation of androgens in human foetal liver in vitro

Zusammenfassung Nach Inkubation des homogenisierten Lebergewebes menschlicher, 7–20 Wochen alter, Foeten mit 3-Hydroxy-5-androsten-17-on wird die Bildung von Testosteron, 4-Androsten-3, 17-dion und 5-Androsten-3, 17-diol nachgewiesen.     

Autoradiographische Befunde an den « direkten » Kerneinschlüssen im Hauptstückepithel der Rattenniere nach experimenteller Bleivergiftung

Summary During a chronic lead intoxication of the white rat, intranuclear inclusions appear in the convoluted tubules of the kidney. Following the administration of H³-cytidine and H³-l-phenylalanine in direct intranuclear inclusions an incorporation of the precursors cannot be observed at all by autoradiography.

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Mit Unterstützung des Bundesministeriums für Wissenschaftliche Forschung.