Structural Changes of Water Molecules Upon the Reduction of Quinones in The Reaction Center from Rhodobactery Sphaeroides

1 Results The photosynthetic bacterial reaction center (RC) is a membrane protein complex.The RC is composed of three protein subunits and redox components such as bacteriochlorophylls, bacteriopheophytins,and quinones.The RC performs the photochemical electron transfer from the bacteriochlorophyll dimer through a series of electron donor and acceptor molecules to a secondary quinone,QB.QB accepts electrons from a primary quinone,QA,in two sequential electron transfer reactions.The second electron trans...     

浅谈随刊附盘的著录与管理

介绍了随刊附盘的种类及其验收与著录的方法,提出了随刊附盘管理和利用的措施。     

升巨散对实验性血小板减少症的治疗作用研究

采用水提醇沉法将升巨散制成冲剂,对实验性血小板减少症的治疗,表明升巨散具有升高外周血小板数量之功效,差异显著(P<0.15).相继在出血时问、凝血时间和24h 血块收缩实验中,也间接证实本品有升高外周血小板数量之作用。又在骨髓象检查中,发现升巨散能提高骨髓之巨核细胞及血小板数量,差异显著。本品 LD_(50)及95%可信限为70.88±2.62g/kg。     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)     

膦酰甲基甘氨酸的制备及其对过氧化氢稳定性的研究

用三氯化磷、甲醛、甘氨酸进行反应制得膦酰甘氨酸（草甘膦），草甘膦对过氧化氢有稳定作用，实验结果表明草甘膦在含０．６％时对过氧化氢具有较好的稳定作用。     

磁场中载流线圈力和力矩的公式

本文给出计算载流线圈在外磁场中所受力和力矩的普遍公式.     

漫谈音乐院校钢琴副科教学的艺术

钢琴副科课是我国音乐院校针对非钢琴专业的学生开设的一门钢琴课程.本文以如何上好钢琴副科课为探讨的主要目的,从基础和提高视谱能力这两个角度来展开讨论,力图使学生学习更富有热情和效率,对知识的掌握更为主动和全面.     

分子间作用力对制备含有机废弃之水泥浆体影响研究

研究以水泥处理固体有机物时，有机物对水泥水化的影响。以膜流模式说明水份在水泥浆体内传输的现象。水膜存在半干之水泥浆体，其厚度小于100nm，膜流由离分压所驱动，而离分压来自分子间作用力。膜拟结果发现，水泥浆体内局部位置可用以进行水化之水量为Q=-Aslv／6vπ。当有机物添加在水泥浆体内时，会影响Hamaker常数，Aslv因此可能使膜流不稳定或完全抑制膜流，因而使水泥浆体内局部缺水，影响水化进行，造成成品强度降低，影响品质。