Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)     

一种改进的实信号IFFT算法

由于重量轻、体积小、功耗低、功能强的小卫星工作在无人干预的低温 ,高辐射的空间环境中 ,因此星上计算机系统必须满足集成度高、寿命长、可靠性高、抗辐射能力强的要求。针对立体测绘小卫星的特点 ,我们设计了满足上述要求的集集中控制与分布控制优点于一身的星上计算机系统 ,对其可靠性进行了分析 ,并对提高其可靠性应采取的关键技术进行了简要的论述。     

发现一种声波传入内耳的新途径

对一位先天性外耳道缺失的患者调查发现，声波传入内耳，除了正常的骨传导和气传导以外，还存在着一种口鼻—咽—咽鼓管—中耳—内耳途径．     

最小二乘解存在的充分必要条件

本文提出了当观测值的权矩阵为正定或半正定的前提下，其最小二乘解存在的充分必要条件为并以间接平差为例验证了该结论的正确性。     

漫谈音乐院校钢琴副科教学的艺术

钢琴副科课是我国音乐院校针对非钢琴专业的学生开设的一门钢琴课程.本文以如何上好钢琴副科课为探讨的主要目的,从基础和提高视谱能力这两个角度来展开讨论,力图使学生学习更富有热情和效率,对知识的掌握更为主动和全面.     

An Improved S-Random Interleaver Design for Turbo Code Based on Chaos Model

A new method to design interleaver based on Henon chaos model is presented. The designed interleaver is with rather random behavior. Experimental results show that the proposed S-henon interleaver make a magnitude of im provement in bit error rate （BER） performance by 0.4 dB compared with the S-random interleaver for AWGN channel respectively. The S-henon interleaver may be adapted to 3G mobile communication systems.     

初中生学习化学兴趣的发展趋势

针对目前初中化学教学中学生因缺乏应有的学习兴趣而导致学习动力不足和影响其个性和谐发展的问题，运用现代教育学、心理学的理论，结合初中生的心理特点和化学学科特点，提出把初中生学习化学兴趣提到学生稳定的个性心理品质上来研究，是符合马克思主义关于人的学说和现代教育心理学理论以及初中化学教学实际的；初中生学习化学兴趣的形成和发展是一个立体发生的过程，其纵向趋势为：直感兴趣→解析兴趣→探索兴趣；其横向品质为：指向性、持久性、广泛性和有效性，初中生学习化学兴趣水平的提高应当在有效的教学中进行     

氯离子诱导的镍在硝酸溶液中的电流振荡

报道了氯了子诱导的镍在0．5mol.dm^-2硝酸溶液中活化－钝化过渡区电流振荡，在恒电位条件下，随着氯离子浓度的升高或者电极电位的降低，电流振荡由周期性振荡逐渐向非周期性振荡转变，根据实验结果，对该体系的电流振荡机理进行了讨论。     

分子间作用力对制备含有机废弃之水泥浆体影响研究

研究以水泥处理固体有机物时，有机物对水泥水化的影响。以膜流模式说明水份在水泥浆体内传输的现象。水膜存在半干之水泥浆体，其厚度小于100nm，膜流由离分压所驱动，而离分压来自分子间作用力。膜拟结果发现，水泥浆体内局部位置可用以进行水化之水量为Q=-Aslv／6vπ。当有机物添加在水泥浆体内时，会影响Hamaker常数，Aslv因此可能使膜流不稳定或完全抑制膜流，因而使水泥浆体内局部缺水，影响水化进行，造成成品强度降低，影响品质。     

回转式电火花展成加工的计算机仿真

介绍了回转式电火花展成加工的原理及特点，并对该加工方法的成形运动进行较深入的分析，给出了在Delphi6环境中实现加工过程的计算机仿真。