水轮发电机组H∞状态反馈综合最优控制系统

根据实际工程应用的特点，改进了Ｈ_∞状态反馈控制的算法，并给出了一种选择Ｈ_∞权矩阵的方法；以此为基础设计及研究了一种能综合控制水轮发电机组的励磁和导叶开度，集励磁调节器、调速器功能于一体的综合控制系统．结果表明，该控制器在基本不影响系统响应性能的情况下，能明显地改善系统的鲁棒性．     

矩形板极限承载能力的探讨

船舶及钻井平台等的上层建筑和潜水器内部平面舱壁板，大多采用弹塑性设计．考虑到这种舱壁板是一次性承载，为提高舰船或平台的承载能力，减轻重量，以提高经济性和增加有效载荷，采用屈服线分析法设计上述壁板，并从理论计算和试验两个方面对该方法进行了探讨，得到了板的极限承载能力的数据并提出了该设计方法下的安全系数．     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)