Specific binding of antigenic peptides to cell-associated MHC class I molecules

T lymphocytes recognize antigen in the form of peptides that associate with specific alleles of class I or class II major histocompatibility (MHC) molecules. By contrast with the clear MHC allele-specific binding of peptides to purified class II molecules purified solubilized class I molecules either bind relatively poorly or show degenerate specificity. Using photo-affinity labelling, we demonstrate here the specific interaction of peptides with cell-associated MHC class I molecules and show that this involves metabolically active processes.     

Characterization of the rough endoplasmic reticulum ribosome-binding activity.

The rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.     

Genotoxic effects of dithane M-45 on the bone marrow cells of mice in vivo

Genotoxic effects of dithane M-45 were studied on the bone marrow cells of male albino mice (Lacca strain) in vivo. Different doses (30 mg, 40 mg and 300 mg/kg b.wt) of dithane M-45 were injected intraperitoneally and their effects were investigated after time intervals of 1, 2, 5 and 10 days. The chromosomal aberrations observed in the bone marrow cells of male mice after treatment with dithane M-45 were fragments, rings, dicentric chromosomes, terminal chromatid deletions, chromatid gaps and breaks. In addition to these chromosomal aberrations, physiological effects such as uneven stretching of chromatin material, end-to-end chromosomal associations, exchange configurations, clumping, stickiness and centromeric associations were also observed.     

大学生口算能力的实验研究

为考察大学生的口算能力,我们随机选取30名大学生为被试进行口算的实验研究,主要结论如下:(1)大学生的口算速率在加、减、乘、除法之间存在差异,从快到慢依次为:除法,借位减法,不借位减法,不进位加法,进位加法,乘法;(2)口算的广度从大到小依次为:不进位的加法广度,进位加法广度,不借位减法广度,借位减法广度,除法广度,乘法广度;(3)大学生口算的速度和广度既无显著的专业差异,也无显著的性别差异.     

秋水仙素诱导青虾次级卵母细胞二倍体初探

以青虾卵巢为材料，采用秋水仙素诱导多倍体次级卵母细胞．经滴片试验，确立此实验最佳滴片高度为1．5cm，通过设置5个秋水仙素浓度(0．08～0．40mg／mL)及3个作用时间(6～24h)，观察发生染色体加倍的细胞相对数目．实验结果表明：在使用秋水仙素的浓度为0．08～0．40mg／mL，处理6～24h时都可以获得多倍体卵细胞．在秋水仙素浓度是0．32mg／mL，处理6h可得到最高二倍体突变率(32．58％)．还测试了0．32mg／mL COM作用6h时ATPase活性与生理盐水对照组的变化，结果显示COM会在一定程度上影响细胞的新陈代谢．但是50％左右的成活率表明，这种诱导突变的方法仍具有一定的应用价值．     

创造性思维的系统化存在

创造性思维的被激发以及被实施的整个过程可视为特定系统。这个系统从基础逻辑认知开始，经由灵感和直觉等非逻辑思维形式，最终被逻辑思维整合。     

ADO意义.NET在并发控制方面存在的问题及解决方案

针对ADO.NET在处理"丢失的修改"问题时,不支持"关键字和已修改字段"的并发控制问题提出了相应的解决办法;同时对ADO.NET架构本身带来的异常更新问题提供了解决思路.     

对英语专业八级口语口译考试评分的探讨

本文通过对英语专业八级口语口译考试的评分标准的考察，指出八级口语口译考试评分中两个突出的特点，即突出口语考试和口译考试评分“信息单元化”，从而影响口译质量评估的有效性。适当改变评分比例和口译评分标准的改进将有助于改善测评效果。