Chromatin fluorescence by pyronin staining

Human, chicken and mouse cells from different tissues show a bright red-orange fluorescence of the chromatin after staining with pyronin Y. The possibility that intercalation of the dye into double helical nucleic acids accounts for this fluorescence pattern is briefly discussed.     

From forecasting to ‘la prospective’ a new way of looking at futures

Although‘La Prospective’is not well known in the Anglo-Saxon forecasting literature, it has been for many years widely used in France and other Latin countries with considerable success. Lately, because of the inaccuracy of forecasting and the large forecasting errors that have been experienced, it is suggested that the Prospective approach can be used as a way of dealing with these problems. The main characteristics of‘La Prospective’are that it does not look at the future as a continuation of the past but rather as the outcome of the wishes of various actors and the constraints imposed on them by the environment. Its purpose is to assist in creating alternative futures and then select some alternative that allows for maximum freedom of action.     

Vesicles carrying nuclear material in matureCyprinus carpio erythrocytes

Zusammenfassung Chromatinhaltige feulgenpositive Bläschen wurden im Cytoplasma der Erythrocyten vonCyprinus carpio gefunden. Sie haben ihren Ursprung in den unmittelbar an der Kernmembran anliegenden Mitochondrien, die während der Chromatinaufnahme allmählich ihre Struktur verlieren.

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The authors wish to thank Mrs.Vera Mondin Weisz and Mr.C. A. Gonçalves Silva for their technical assistance and Mrs.Sibylle Heller for her editorial aid and translation. This research has been supported by the Conselho Nacional de Pesquisas (Proc. No. 10112/71) and Fundo Especial de Despesas do Instituto Butantan.     

The turnover of F-actin-bound ADP in vivo.

A procedure for estimating the rate of turnover of F-actin-bound ADP in vivo is described. A turnover rate of 0.88 h-1 was determined for mouse muscle F-actin. The validity of the method when used to estimate the turnover rate of F-actin per se is discussed in relation to the possible exchange of F-actin-bound ADP.