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131.
Plant biomarkers in aerosols record isotopic discrimination of terrestrial photosynthesis 总被引:3,自引:0,他引:3
Carbon uptake by the oceans and by the terrestrial biosphere can be partitioned using changes in the (12)C/(13)C isotopic ratio (delta(13)C) of atmospheric carbon dioxide, because terrestrial photosynthesis strongly discriminates against (13)CO(2), whereas ocean uptake does not. This approach depends on accurate estimates of the carbon isotopic discrimination of terrestrial photosynthesis (Delta; ref. 5) at large regional scales, yet terrestrial ecosystem heterogeneity makes such estimates problematic. Here we show that ablated plant wax compounds in continental air masses can be used to estimate Delta over large spatial scales and at less than monthly temporal resolution. We measured plant waxes in continental air masses advected to Bermuda, which are mainly of North American origin, and used the wax isotopic composition to estimate Delta simply. Our estimates indicate a large (5 6 per thousand) seasonal variation in Delta of the temperate North American biosphere, with maximum discrimination occurring in late spring, coincident with the onset of production. We suggest that the observed seasonality arises from several factors, including seasonal shifts in the proportions of production by C(3) and C(4) plants, and environmentally controlled adjustments in the photosynthetic discrimination of C(3)-plant-dominated ecosystems. 相似文献
132.
Hall N Pain A Berriman M Churcher C Harris B Harris D Mungall K Bowman S Atkin R Baker S Barron A Brooks K Buckee CO Burrows C Cherevach I Chillingworth C Chillingworth T Christodoulou Z Clark L Clark R Corton C Cronin A Davies R Davis P Dear P Dearden F Doggett J Feltwell T Goble A Goodhead I Gwilliam R Hamlin N Hance Z Harper D Hauser H Hornsby T Holroyd S Horrocks P Humphray S Jagels K James KD Johnson D Kerhornou A Knights A Konfortov B Kyes S Larke N Lawson D Lennard N Line A Maddison M 《Nature》2002,419(6906):527-531
Since the sequencing of the first two chromosomes of the malaria parasite, Plasmodium falciparum, there has been a concerted effort to sequence and assemble the entire genome of this organism. Here we report the sequence of chromosomes 1, 3-9 and 13 of P. falciparum clone 3D7--these chromosomes account for approximately 55% of the total genome. We describe the methods used to map, sequence and annotate these chromosomes. By comparing our assemblies with the optical map, we indicate the completeness of the resulting sequence. During annotation, we assign Gene Ontology terms to the predicted gene products, and observe clustering of some malaria-specific terms to specific chromosomes. We identify a highly conserved sequence element found in the intergenic region of internal var genes that is not associated with their telomeric counterparts. 相似文献
133.
Massive gene decay in the leprosy bacillus 总被引:73,自引:0,他引:73
Cole ST Eiglmeier K Parkhill J James KD Thomson NR Wheeler PR Honoré N Garnier T Churcher C Harris D Mungall K Basham D Brown D Chillingworth T Connor R Davies RM Devlin K Duthoy S Feltwell T Fraser A Hamlin N Holroyd S Hornsby T Jagels K Lacroix C Maclean J Moule S Murphy L Oliver K Quail MA Rajandream MA Rutherford KM Rutter S Seeger K Simon S Simmonds M Skelton J Squares R Squares S Stevens K Taylor K Whitehead S Woodward JR Barrell BG 《Nature》2001,409(6823):1007-1011
Leprosy, a chronic human neurological disease, results from infection with the obligate intracellular pathogen Mycobacterium leprae, a close relative of the tubercle bacillus. Mycobacterium leprae has the longest doubling time of all known bacteria and has thwarted every effort at culture in the laboratory. Comparing the 3.27-megabase (Mb) genome sequence of an armadillo-derived Indian isolate of the leprosy bacillus with that of Mycobacterium tuberculosis (4.41 Mb) provides clear explanations for these properties and reveals an extreme case of reductive evolution. Less than half of the genome contains functional genes but pseudogenes, with intact counterparts in M. tuberculosis, abound. Genome downsizing and the current mosaic arrangement appear to have resulted from extensive recombination events between dispersed repetitive sequences. Gene deletion and decay have eliminated many important metabolic activities including siderophore production, part of the oxidative and most of the microaerophilic and anaerobic respiratory chains, and numerous catabolic systems and their regulatory circuits. 相似文献
134.
Enhanced expression of H-2K and H-2D antigens on reticulocytes infected with Plasmodium yoelii 总被引:6,自引:0,他引:6
The 17XNL strain of Plasmodium yoelii induces a highly effective and permanent T-cell dependent immunity in mice of the CBA strain; the lethal variant P. yoelii 17XL and P. berghei (ANKA) fail to activate an effective immune response in the same host. These differences in immunogenicity are unexplained. We recently observed that in CBA/CaJ mice the intracellular blood stages of P. yoelii 17XNL were almost exclusively within reticulocytes whereas lethal P. yoelii 17XL and P. berghei (ANKA), at comparable stages of infection, were predominantly erythrocytic. Induction of a reticulocytosis converted the normally lethal P. yoelii 17XL infection into a nonlethal one, and reticulocytic P. yoelii was shown to be more immunogenic than the erythrocytic form. Since one of the differences between reticulocytes and erythrocytes that might have influenced the development of immunity was greater expression of MHC antigens of the former cell type we examined the expression of H-2K, H-2D and Ia on reticulocytes infected with P. yoelii 17XNL. These cells showed a very marked increase in H-2K and D antigen expression compared to normal reticulocytes or erythrocytes. No Ia was detected. Red blood cells (RBC) infected with lethal P. yoelii 17XL or P. berghei showed no increase in H-2K or H-2D antigen expression. Finally, the level of expression of H-2K on P. yoelii 17XNL parasitized red blood cells from different strains of mice correlated closely with the ability of these strains to control the infection. 相似文献
135.
136.
D. E. Redmond Jr. J. Baulu D. L. Murphy 《Cellular and molecular life sciences : CMLS》1976,32(9):1213-1215
Summary Plasma amine oxidase activity increased from 23.4 nmol/ml/h during pregnancy to 49.5 nmol/ml/h during an extended post partum period in 10 rhesus monkeys. Comparison with non-pregnant control monkeys sampled at similar times indicated that the significant differences were in the extended post partum period.We thank Dr.C. H. Conaway and Dr.J. D. Loy of the Caribbean Primate Research Center andJ. G. Vandenbergh andW. Post of the North Carolina Department of Mental Health for the use of their facilities and animals. We also acknowledge the technical assistance ofC. H. Donnelly andMs. Suzanne Baulu. This work was supported by a grant from Dr.H. C. Robinson, Jr., through Yale University Medical School and by NIH Contract No. 71-2003. 相似文献
137.
Zusammenfassung Wird bei einem künstlich synchronen Plasmodium des MyxomycetenPhysarum polycephalum die Mitose promitotischer Kerne durch deren Verlagerung in postmitotische (=S phase) Zonen des gleichen Plasmodiums verhindert, so behält die nucleoläre DNS, im Gegensatz zur extranukleolären DNS, weiterhin die Fähigkeit,3H-Thymidin einzubauen. Es wird die Hypothese vorgeschlagen, dass die Synthese der nukleolären DNS einem anderen Kontrollmechanismus unterliegt als die der extranukleolären DNS.
This investigation was supported in part by PHS Research Grant No. GM 18221. 相似文献
This investigation was supported in part by PHS Research Grant No. GM 18221. 相似文献
138.
139.
A fragment of the SV40 large T-antigen gene transforms 总被引:29,自引:0,他引:29
140.
Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity 总被引:20,自引:0,他引:20
A J Docherty A Lyons B J Smith E M Wright P E Stephens T J Harris G Murphy J J Reynolds 《Nature》1985,318(6041):66-69
Collagen fibres form the stable architecture of connective tissues and their breakdown is a key irreversible step in many pathological conditions. The destruction of collagen is usually initiated by proteinases, the best known of which is the metalloproteinase collagenase (EC 3.4.24). Collagenase and related metalloproteinases are regulated at the level of their synthesis and secretion, through the action of specific stimuli such as hormones and cytokines, and also at the level of their extracellular activity through the action of a specific inhibitor, TIMP (tissue inhibitor of metalloproteinases), which irreversibly forms inactive complexes with metalloproteinases. Although the mechanisms governing the production of TIMP are unknown, immunologically identical forms of this glycoprotein have been detected in a wide variety of human body fluids and cell and tissue culture media. We therefore suggested that under physiological conditions this ubiquitous inhibitor predominates over active metalloproteinases and that tissue destruction may arise when any perturbation of this controlling excess arises. However, further progress towards testing this theory has been hindered by a lack of knowledge about the structure of TIMP and insufficient material for studying it in model systems. Here we describe the structure of TIMP predicted from its complementary DNA, its synthesis in Escherichia coli and transfected animal cells, and the finding that it is identical to a protein recently reported to have erythroid-potentiating activity (EPA). 相似文献