Plcd1转基因小鼠的制备

通过制备Plcd1转基因小鼠研究Plcd1基因功能.首先逆转录PCR获得约2.2kb的Plcd1基因全长,T载体克隆后测序验证;以pMD19-T-Plcd1为模板,通过设计引物及PCR扩增引入酶切位点,与pEF6/V5-His同时进行酶切、连接,构建pEF6/V5-His-Plcd1表达载体;经真核表达验证后,酶切获得目标片段,显微注射681枚受精卵,在82只仔鼠中获得转基因阳性首建鼠15只,其中13只稳定遗传并建系.PLCD1-HIS融合蛋白在睾丸组织中表达,在皮肤组织中无表达.Plcd1转基因小鼠为Plcd1功能研究奠定了基础.     

Einfluß antianaphylaktisch wirkender Stoffe auf die Antikörperproduktion

Summary To produce antisera of high antibody concentration animals after sensitization with protein have been reinjected with lethal doses of the antigen, protected against the shock-effect by substances with antihistamin properties (as example Antistin). The antibody concentration was considerably higher in these animals as in the other non-protected group reinjected with the highest tolerated antigen dosage.     

复杂设备运行的协同服务支持

针对现有的设备服务模式存在的问题，提出支持复杂设备优化运行的协同服务模式．为了使复杂设备能充分发挥其潜在价值，指出复杂设备的运行应该与必需的技术服务关联起来，形成“产品服务捆绑体”．解释了支持复杂设备运行的协同服务内涵，并对组织结构形式、协同服务业务模式识别和过程建模关键技术进行系统论述，提出了建立嵌入式在线服务标准（embeddedo nline service，EOS）作为支持协同服务运行的设备接入技术．     

SiC纤维（Nicalon）增强玻璃陶瓷的蠕变行为

考察了文题材料的蠕变行为，发现其以取决于所施加的应力与温度而出现的蠕变第一、第二、第三阶段为特征。蠕变过程中出现的主要结构变化为纤维重排、富碳界面层的氧化以及基质中晶体的生长。虽然复合材料的受力极限取决于残余玻璃相，但纤维的引入使得材料的热机械性能得以大幅度地提高。     

Bromocriptine/SKF38393 ameliorates islet dysfunction in the diabetic (db/db) mouse

Dysfunction of pancreatic islets plays a crucial role in the etiology of type II diabetes. Chronic hyperglycaemia or hyperlipidaemia may impair islet function. Previous studies by our laboratory have demonstrated that dopaminergic agonists ameliorated hyperglycaemia and hyperlipidaemia in obese and diabetic rodents. In the present study, we investigated the effect of a treatment with the dopamine D₂ /D₁ receptor agonists (bromocriptine/SKF38393, BC/SKF) on islet dysfunction in db/db mice. Our results show that a 2-week BC/SKF treatment markedly reduced hyperglycaemia and hyperlipidaemia, and significantly improved islet dysfunction demonstrated by an increase of secretagogue-stimulated insulin release from islets of db/db mice to levels observed in islets from lean mice. There was also a fourfold increase of insulin content in the pancreas of BC/SKF-treated db/db mice compared with that in untreated controls. The effect of BC/SKF on islet function cannot be mimicked in pair-fed animals. BC/SKF had no direct stimulatory effect on islet insulin secretion, suggesting BC/SKF treatment improved islet function via an indirect mechanism. This treatment markedly improved the abnormally elevated daily levels of corticosterone, blood glucose and plasma lipids, supporting the view that BC/SKF may affect the neuroendocrine system that in turn regulates peripheral metabolism and thereby improves islet function. Received 3 April 1998; accepted 27 April 1998     

Expression of endogenous murine leukaemia viruses in AKR/J streaker mice

MICE of the AKR/J strain show high rates of spontaneous thymic lymphoma and are chronically infected with murine leukaemia viruses. Total thymectomy in young mice of this strain results in a marked decrease in the incidence of leukaemia. The finding of athymic mutant mice on the AKR/J background (referred to as streaker mice) is of importance in the study of genetic factors which influence virus expression and leukaemogenesis. Here we report that mice of both normal and mutant genotypes vary in the expression of a particular class of murine leukaemia virus. This lower virus expression in conjunction with the absence of a thymus leads to a lower tumour incidence in streaker mice.     

Zusammenhang zwischen Konzentration der 7-Methylbisdehydrodoisynolsäure im Uterus und ihrem Wachstumseffekt

Summary After administration of methylbisdehydrodoisynolic acid (1-ethyl-2-methyl-7-methoxy-tetrahydro-phenantryl-2-carbonic acid) the content of this oestrogen in the uterus of the spayed rat has been determined. It has been found, that a concentration of 3–4/100 g uterus tissue is necessary for the growth reaction of the organ. The concentration in the uterus lies between the concentration of muscle and liver. So the specific sensitivity of the uterus must be responsable for his reaction to an oestrogen.     

Protective action of irradiated bone marrow cells

Zusammenfassung Wurdein vitro röntgenbestrahltes Knochenmark (475r, 950 r, 1425 r) in letal bestrahlte Mäuse (LD 100/11=950 r) injiziert, so ergab sich: Isologes Knochenmark, mit 950 r bestrahlt, besitzt noch beachtliche Schutzwirkung; bestrahltes homologes und heterologes Knochenmark war weniger oder ganz inaktiv.