Regularization strategy for the Cauchy problem of Laplace’s equation from the viewpoint of regularization theory

Within the framework of the regularization theory, a spectral regularization method is introduced and analyzed. The convergence estimate under an appropriate choice of regularization parameter is obtained. A numerical implementation is described. Numerical examples show that the proposed method is effective and stable.     

Cyclin-dependent kinases prevent DNA re-replication through multiple mechanisms.

The stable propagation of genetic information requires that the entire genome of an organism be faithfully replicated once and only once each cell cycle. In eukaryotes, this replication is initiated at hundreds to thousands of replication origins distributed over the genome, each of which must be prohibited from re-initiating DNA replication within every cell cycle. How cells prevent re-initiation has been a long-standing question in cell biology. In several eukaryotes, cyclin-dependent kinases (CDKs) have been implicated in promoting the block to re-initiation, but exactly how they perform this function is unclear. Here we show that B-type CDKs in Saccharomyces cerevisiae prevent re-initiation through multiple overlapping mechanisms, including phosphorylation of the origin recognition complex (ORC), downregulation of Cdc6 activity, and nuclear exclusion of the Mcm2-7 complex. Only when all three inhibitory pathways are disrupted do origins re-initiate DNA replication in G2/M cells. These studies show that each of these three independent mechanisms of regulation is functionally important.     

渣油热接触改质反应机理——Ⅰ.不同热载体反立性能的考察

采用X光衍射、程序升温脱附、扫描电镜、微活性测试及表面结构测试等方法,对不同热载体的性能与结构进行了考察。结果表明,热载体是一种具有低比表面、低催化活性的情性物质,孔径范围大多在15～60nm,热载体的孔结构类似于平行壁的狭缝状毛细孔结构。用胜利常压渣油作原料,在固定流化床反应器中考察不同热载体的反应性能,发现热载体结构对反应结果有很大影响,同时,热载体还有较强的抗重金属的污染能力。     

渣油热接触改质反应机理——Ⅱ.胶质和沥青质的转化

以添加不同数量胶质或沥青质的胜利减二线为原料油,采用2~#热载体,在固定流化床反应装置上考察了胶质、沥青质的转化。结果表明,在通常的反应条件下,胶质大部分转化为液体,沥青质大部分转化为焦炭。采用热接触改质,可脱除渣油中40～60%的S、N,90%左右重金属Ni以及85%以上的残炭,约90%的胶质和近乎100%的沥青质。     

基于造影图对的血管三维重建显示

在某些医学诊断和治疗过程中需要了解生理结构的三维信息,本文介绍了血管三维重建的工作.首先讨论了几种处理方法,着重讨论三维重建的匹配过程,并提出了一种新的基于二叉树数据结构的匹配方法.该方法能有效地解决匹配过程中一点对应多点的问题.最后介绍了一种对血管进行三维描述和显示的技术.     

数值积分的padé逼近方法

本文将Pade′逼近方法应用于定积分的数值计算。讨论了收敛性,给出了若干近似计算公式。当应用这些公式计算某些定积分时,得到了较为精确的结果。     

履带车辆软地转向性能理论分析

建立了一个履带车辆软地稳态转向模型,模型中考虑到了车辆沉陷、纵向倾角等因素;对模型参数进行了数值计算,并以此分析了土壤、车辆结构诸多参数对转向性能的影响.     

尿激酶粗制品的提取技术

本文在叙述尿激酶的药用价值及发现历程的同时,结合生产实践,提供了以硅胶-G及celitet—545这两种不同吸附剂提取尿激酶粗品的方法。     

立体预测中的地质几何方法的原理和应用

本文研究了当前正在发展的立体定量预测中的地质几何方法的基本原理和数学模型及其应用。作者采用多项式函数和趋势-剩余分析对控矿地质界面(如地层界面、不整合面、断裂面及侵入接触界面等)进行形态模拟,并根据模拟所得地质几何参数对矿体进行空间定位和深边部立体预测,然后采用三维趋势分析或克里金法对预测地段的矿床值进行定量估计。四个矿床的预测应用证明该方法实际效果较好。     

Preferential DNA secondary structure mutagenesis in the lagging strand of replication in E. coli

When present in single-stranded DNA, palindromic or quasi-palindromic sequences have the potential to form complex secondary structures, including hairpins, which may facilitate interstrand misalignment of direct repeats and be responsible for diverse types of replication-based mutations, including deletions, additions, frameshifts and duplications. In regions of palindromic symmetry, specific deletion events may involve the formation of a hairpin or other DNA secondary structures which can stabilize the misalignment of direct repeats. One model suggests that these deletions occur during DNA replication by slippage of the template strand and misalignment with the progeny strand. The concurrent DNA replication model, involving an asymmetric dimeric DNA polymerase III complex which replicates the leading and lagging strands, has significant implications for mutagenesis. The intermittent looping of the lagging strand template, and the fact that the lagging strand template may contain a region of single-stranded DNA the length of an Okazaki fragment, provides an opportunity for DNA secondary-structure formation and misalignment. Here we report our design of a palindromic fragment to create an 'asymmetric palindromic insert' in the chloramphenicol acetyltransferase gene of plasmid pBR325. The frequency with which the insert was deleted in Escherichia coli depends on the orientation of the gene in the plasmid. Our results suggest that replication-dependent deletion between direct repeats may occur preferentially in the lagging strand.