Growth hormone receptor and serum binding protein: purification, cloning and expression

A putative growth hormone receptor from rabbit liver and the growth hormone binding protein from rabbit serum have the same amino-terminal amino-acid sequence, indicating that the binding protein corresponds to the extracellular hormone-binding domain of the liver receptor. The complete amino-acid sequences derived from complementary DNA clones encoding the putative human and rabbit growth hormone receptors are not similar to other known proteins, demonstrating a new class of transmembrane receptors.     

更好地理解量子理论的新方法

采用与通常教科书不同的方式,讨论了量子力学中的一些对初学者很难理解的概念,如量子化、对易关系、用矩阵表示力学量、引进复数i等.解决在实轴上“挖洞”、映射到实数以及找出作为动力学变量的生成元,都是与对上述概念的理解相联系的.     

Dependence of 6β-acetoxy-7α-hydroxyroyleanone block of Kv1.2 channels on C-type inactivation

Voltage-gated K⁺ (Kv) channels exhibit slow or C-type inactivation during continuous depolarization. A selective pharmacological agent targeting C-type inactivation is hitherto lacking. Here, we report that 6β-acetoxy-7α-hydroxyroyleanone (AHR), a diterpenoid compound isolated from Taiwania cryptomerioides, can selectively modify C-type inactivation of Kv1.2 channels. Extracellular, but not intracellular, AHR (50 μM) dramatically accelerated the slow decay of Kv currents and left-shifted the steady-state inactivation curve. AHR blocked Kv currents with an IC₅₀ of 17.7 μM. AHR did not affect the kinetics and voltage-dependence of Kv1.2 channel activation. Channel block by AHR was independent of intracellular K⁺ concentration. In addition, effect of AHR was much attenuated in a Kv1.2 V370G mutant defective in C-type inactivation. Therefore, block of Kv1.2 channels by AHR did not appear to involve direct occlusion of the outer pore but depended on C-type inactivation. AHR could thus be a probe targeting Kv channel C-type inactivation gate.     

Theoretical Analysis of the Factors Affecting Splice Loss During Fabric Spreading

Material utilization is mainly concerned by industrialpractitioners since it contributes to minimize the totalproduction costs for garment manufacturing. Apart fromthe marker loss, splice loss (the fabric loss during thejoining of different fabric rolls) is one of the primarycauses of fabric loss, but it has not been considered spe-cifically in the past literatures. The amount of splice lossduring fabric spreading is governed by various factors in-cluding the marker length, the number of fabric rolls,and the number of splicing intervals for laying-up. Inour study, these three effects on splice loss during fabricspreading were computed and analysed.     

MATRIX-AGENT FRAMEWORK： A VIRTUAL PLATFORM FOR MULTI-AGENTS

1. Introduction The system development life cycle in software engineering has gradually moved from traditional pipelining-like processes towards more open and flexible processes. Multi-agent technology has become one of the major directions of future software engineering development as it has the capacity to play a key role in current and future computer science and its application (Weiss, 1999). In the modern business and technology environment, organisational information systemsare facing co…     

高延性永久模板在建造耐久混凝土结构中的应用

介绍了高延性水泥基复合材料(PDCC)永久性模板在建造耐久混凝土结构中的应用.在预浇筑的PDCC永久性模板上浇筑普通混凝土,两者共同组成结构构件.低水灰比永久性模板具有的低渗透性和抗开裂能力使其成为防止钢筋锈蚀的有效保护层.在初步应用上有2种途径:使用素PDCC基体制作永久性模板和使用玻璃纤维增强塑料筋(GFRP reinforcement)加固PDCC模板从而为实际构件提供有效的承载力.进一步的应用可在构件中配合使用GFRP Bar和钢筋从而同时获得足够的承载力和延性破坏形式.介绍了PDCC永久性模板的制作过程和力学性能,比较了在不同表面处理方法下模板与普通混凝土之间的粘结力,并且分析了使用GFRP Bar加固模板制作构件的4点弯曲的理论计算和实验结果.初步的实验结果显示了该技术在实际应用上的广阔前景.     

Investigation on the Consumption Values of Hong Kong People on Tie Products

Introduction Overtheyears,comparingwithanyotheraccessoinmale swardrobe,necktieisregardedasthestron indicationtoaman spersonalityandthesoulforthem fashion.Inretailbusiness,certainly,accessoriesoffe greatopportunityforadd onsales.Asthefirstkncravat(referredtotodayasthenecktie)evolved,itt onnumerousshapes,fabricdesigns,specialeffects colours.Yet,thenecktieremainsanessentialparto man swardrobebecauseitunitesalltheelementsofam outfit,giveshiminstantrespectabilityand,aboveall, theultimatesymbolofi…     

Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer

Bacteriophage Mu is the largest and most efficient transposable element known. The Mu transposase (A protein) of relative molecular mass 75,000 is a central component of the transposition machinery. We report here that the N-terminal region of Mu transposase contains two distinct DNA-binding domains, one which binds the two Mu DNA ends, and another which binds an internal operator region. This internal operator is required for the transposase-mediated synapsis and nicking of Mu ends in vitro, and stimulates transposition more than 100-fold in vivo. The orientation of the operator with respect to the ends is critical to its function, whereas its distance from the ends seems to be relatively unimportant. We propose that the operator enhances transposition by transiently interacting with the transposase and Mu DNA end(s) to form a complex in which synapsis of the ends occurs.     

A new approach to forecasting intermittent demand based on the mixed zero-truncated Poisson model

This paper proposes a new approach to forecasting intermittent demand by considering the effects of external factors. We classify intermittent demand data into two parts—zero value and nonzero value—and fit nonzero values into a mixed zero-truncated Poisson model. All the parameters in this model are obtained by an EM algorithm, which regards external factors as independent variables of a logistic regression model and log-linear regression model. We then calculate the probability of occurrence of zero value at each period and predict demand occurrence by comparing it with critical value. When demand occurs, we use the weighted average of the mixed zero-truncated Poisson model as predicted nonzero demands, which are combined with predicted demand occurrences to form the final forecasting demand series. Two performance measures are developed to assess the forecasting methods. By presenting a case study of electric power material from the State Grid Shanghai Electric Power Company in China, we show that our approach provides greater accuracy in forecasting than the Poisson model, the hurdle shifted Poisson model, the hurdle Poisson model, and Croston's method.