Comparative Study on Shearing Edge Quality Influence of the Change of Tool Geometry in Fine-Blanking for Non-homogeneous Materials

Excellent quality of shearing edge implies that a s mo oth cutting edge without tearing will be observed on the whole edge surface. Thi s is one of the most significant features of the Fine-blanking process. To achi eve such a superb blanking edge quality in fine-blanking, there actually involv es quite a large number of factors, such as blanking speed, processing material, product shape, lubrication and tool geometry, to be considered simultaneously d uring the operation. Nevertheless, the thorough invest...     

A high–low model of daily stock price ranges

We observe that daily highs and lows of stock prices do not diverge over time and, hence, adopt the cointegration concept and the related vector error correction model (VECM) to model the daily high, the daily low, and the associated daily range data. The in‐sample results attest to the importance of incorporating high–low interactions in modeling the range variable. In evaluating the out‐of‐sample forecast performance using both mean‐squared forecast error and direction of change criteria, it is found that the VECM‐based low and high forecasts offer some advantages over alternative forecasts. The VECM‐based range forecasts, on the other hand, do not always dominate—the forecast rankings depend on the choice of evaluation criterion and the variables being forecast. Copyright © 2008 John Wiley & Sons, Ltd.     

The gene for theta-globin is transcribed in human fetal erythroid tissues

A new gene like the alpha-globin gene has been identified in higher primates at the 3' end of the alpha-globin gene cluster. There is some controversy as to whether this gene, theta, is a functional globin gene or a non-functional pseudogene. The high degree of sequence conservation displayed by theta between primates and various mammals, such as horse and rabbit, suggests that this gene is functional in some species. Furthermore, theta encodes a 141-amino-acid polypeptide in sequence similar to alpha-globin and appears to possess functional RNA-processing signals. But the promoter region of theta is unlike the other globin genes because its CCAAT and ATA box sequences are displaced from the coding sequence by the insertion of a 200-base-pair GC-rich sequence. We demonstrate here the presence of theta-globin messenger RNA in human fetal erythroid tissue, but not in adult erythroid or other non-erythroid tissues. Furthermore, theta-globin mRNA is detectable in significant amounts in a human erythroleukaemic cell line. These results predict that theta-globin protein will be found in the early stages of human fetal development. Surprisingly, the promoter sequence of theta-globin does not correspond to the CCAAT and ATA box sequences of the gene but rather lies within the adjacent GC-rich sequence, resulting in a heterogeneous series of mRNA 5' ends 50-10 base pairs to 5' of the initiation codon. This type of promoter is reminiscent of that found in housekeeping genes such as adenine deaminase and hypoxanthine-guanine phosphoribosyl-transferase.     

Nucleolar proteome dynamics

The nucleolus is a key organelle that coordinates the synthesis and assembly of ribosomal subunits and forms in the nucleus around the repeated ribosomal gene clusters. Because the production of ribosomes is a major metabolic activity, the function of the nucleolus is tightly linked to cell growth and proliferation, and recent data suggest that the nucleolus also plays an important role in cell-cycle regulation, senescence and stress responses. Here, using mass-spectrometry-based organellar proteomics and stable isotope labelling, we perform a quantitative analysis of the proteome of human nucleoli. In vivo fluorescent imaging techniques are directly compared to endogenous protein changes measured by proteomics. We characterize the flux of 489 endogenous nucleolar proteins in response to three different metabolic inhibitors that each affect nucleolar morphology. Proteins that are stably associated, such as RNA polymerase I subunits and small nuclear ribonucleoprotein particle complexes, exit from or accumulate in the nucleolus with similar kinetics, whereas protein components of the large and small ribosomal subunits leave the nucleolus with markedly different kinetics. The data establish a quantitative proteomic approach for the temporal characterization of protein flux through cellular organelles and demonstrate that the nucleolar proteome changes significantly over time in response to changes in cellular growth conditions.     

Bmi1 is essential for cerebellar development and is overexpressed in human medulloblastomas

Overexpression of the polycomb group gene Bmi1 promotes cell proliferation and induces leukaemia through repression of Cdkn2a (also known as ink4a/Arf) tumour suppressors. Conversely, loss of Bmi1 leads to haematological defects and severe progressive neurological abnormalities in which de-repression of the ink4a/Arf locus is critically implicated. Here, we show that Bmi1 is strongly expressed in proliferating cerebellar precursor cells in mice and humans. Using Bmi1-null mice we demonstrate a crucial role for Bmi1 in clonal expansion of granule cell precursors both in vivo and in vitro. Deregulated proliferation of these progenitor cells, by activation of the sonic hedgehog (Shh) pathway, leads to medulloblastoma development. We also demonstrate linked overexpression of BMI1 and patched (PTCH), suggestive of SHH pathway activation, in a substantial fraction of primary human medulloblastomas. Together with the rapid induction of Bmi1 expression on addition of Shh or on overexpression of the Shh target Gli1 in cerebellar granule cell cultures, these findings implicate BMI1 overexpression as an alternative or additive mechanism in the pathogenesis of medulloblastomas, and highlight a role for Bmi1-containing polycomb complexes in proliferation of cerebellar precursor cells.     

正交各向异性板Ⅲ型裂纹问题的William′s一般解

引入参数β=μx/μy,将正交各向异性板反平面裂纹问题的基本边值问题转换为正交各向同性的形式,反平面裂纹问题的位移和应力在正交各向同性和正交各向异性这两种情况之间的比拟关系非常简单,使问题的求解更为方便.为了说明这个比拟方法,分别求导了含有内部裂纹和边缘裂纹的正交各向异性板Ⅲ型二维裂纹问题的William′s一般解.这些William′s一般解对于用FFEM和其他数值方法来求解正交各向异性板反平面裂纹问题是一个非常重要的基础.研究结果表明这种比拟变换方法能有效地简化正交各向异性板Ⅲ型裂纹问题的求解.     

Effect of Fabric Elongation on the Making Up of Pressure Garments

Pressure garments are widely used in Hong Kong andmany other countries for burn rehabilitation.Thesegarments are mainly made of elastic Lycra fabrics andtailor-made to individual patient's measurement toprovide an appropriate amount of skin-garment in-terface pressure for medical treatment.However,thefabric tension would be reduced due to fabric elonga-tion under prolonged period of stress,and thus theskin-garment interface pressure cannot be main-tained after repeated use of the garment.This paperaims to study the behaviour of fabric elongation of thefabrics commonly used for making pressure garmentsin U.K.and/or Hong Kong.Attempts to correct theexisting practice of drafting the pressure garments forproviding a more effective clinical treatment.