Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system

Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only inO. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect inO. danica were judged comparable to those found in animal cells whereas the resultsE. coli were not entirely equivalent. TheO. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.     

(−)-Epigallocatechin gallate,a polyphenolic tea antioxidant,inhibits peroxynitritemediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine

Reaction with peroxynitrite at pH 7.4 and 37°C was found to increase the 8-oxodeoxyguanosine levels in calf thymus DNA 35-38-fold. This oxidation of deoxyguanosine, as well as the peroxynitrite-mediated nitration of tyrosine to 3-nitrotyrosine, was significantly inhibited by ascorbic acid, glutathione and (–)-epigallocatechin gallate, a polyphenolic antioxidant present in tea. For 50% inhibition of the oxidation of deoxyguanosine to 8-oxodeoxyguanosine, 1.1, 7.6 of 0.25 mM ascorbate, glutathione or (–)-epigallocatechin gallate, respectively, was required. For 50% inhibition of tyrosine nitration, the respective concentrations were 1.4, 4.6 or 0.11 mM. Thus, (–)-epigallocatechin gallate is a significantly better inhibitor of both reactions than either ascorbate or glutathione. Reaction of (–)-epigallocatechin gallate with peroxynitrite alone resulted in the formation of a number of products. Ultraviolet spectra of two of these suggest that the tea polyphenol and/or its oxidation products are nitrated by peroxynitrite.     

Growth ages of ferromanganese crusts from the western and central Pacific： Comparison between nannofossil analysis and ^10Be dating

Based on results of nannofossil analysis and 10Be dating in ferromanganese crusts M1-1 and A1-1 (no nannofossils were found in it),from the western and central Pacific respectively,it is found that the crust growth ages from nannofossil biostrati-graphy agree well with those based on 10Be isotope analysis. Both crusts have three growth layers,and the oldest layer was deposited in Miocene at about 12.80 Ma. The maximum,minimum,and average growth rates of crust A1-1 (from the central Pacific) are 8.11,1.92 and 3.47 mm/Ma,and those of crust M1-1 (from the western Pacific) are 2.93,0.47,and 0.94 mm/Ma.     

Growth of Magnetite Nanoparticles under Magnetic Fields

1 Introduction Over the past several years, the preparation and characterization ofnanoscale magnetic materials, especially one-dimensional (1D) nanostructure, have attracted much attention as the nanomaterials would allow investigating the fundamental aspects of magnetic-ordering phenomena in magnetic materials with reduced dimensions and could lead to new potential applications such as data storage technology[1-6].     

略论唐朝治桂政策实施的背景

唐朝以前，桂东地区的封建庄园经济。土地私有制已有所发展，而桂西地区大体上仍停留在前封建制阶段，社会经济相当落后，尤其是农业经济与桂东有着相当大的差距。唐朝统治者审时度势，根据当时广西社会发展的变化，调整了其治桂政策，在桂东地区设立正州正县，而在桂西地区则继续沿袭前代的的羁縻政策。     

MERGING OPTIMALITY CONDITIONS WITH GENETIC ALGORITHM OPERATORS TO SOLVE SINGLE MACHINE TOTAL WEIGHTED TARDINESS PROBLEM

1.Introduction Production sequencing and scheduling is one of the most important activities in production planning and control.Sequencing is defined as the order in which the jobs are processed through the machines.The allocation of machines over time to process a collection of jobs is defined as Scheduling.Several methods have been developed to solve the scheduling problem which can be classified as follows:1)efficient optimal methods,2)implicit and explicit,or complete enumerative methods,a…     

低温等离子体促进水稻种子生长效应及NIR光谱分析

使用亚大气压下介质阻挡放电低温等离子体,对水稻糙米种子进行处理,不同气体、功率、处理时间下观测低温等离子体对糙米生长活力的影响;采用近红外光谱技术对处理后的糙米进行初步的理化性质分析.结果显示:空气等离子体放电对糙米生长活力的促进要优于氮气等离子体放电,空气实验组的最佳处理条件为(360W,5min),而氮气实验组的最佳处理条件为(360W,10min).另外,近红外光谱的主成分分析结果显示空气实验组(360W,5min)和氮气实验组(360W,10min)的糙米光谱与对照组光谱均存在明显差异.低温等离子体对水稻糙米种子的生长活力具有促进作用,能利用近红外光谱技术对作用效果进行初步评估.     

Effect of cold rolling process on microstructure and mechanical properties of high strength β titanium alloy thin sheets

The microstructure and mechanical properties of Ti-3.5Al-5Mo-6V-3Cr-2Sn-0.5Fe high strength titanium alloy sheets prepared by unidirectional cold rolling and two-step cross cold rolling were investigated. Results showed that the β phase grains were refined significantly by cold rolling followed by solution treatment for a short time.Compared to unidirectional cold rolling, the short time solution treatment after two-step cross rolling could significantly reduce the non-uniformity of the microstructure of the alloy sheets. After aging treatment at 550 ℃,the anisotropy of the mechanical properties still existed in the unidirectional rolled sheets, and the tensile strength was highest along the rolling direction. After solution and aging treatment, the anisotropy of the mechanical properties of the two-step cross rolling process sheet was not obvious than unidirectional cold rolling,and alloy had good strength and plasticity matching.     

Minimum information about a microarray experiment (MIAME)-toward standards for microarray data.

Microarray analysis has become a widely used tool for the generation of gene expression data on a genomic scale. Although many significant results have been derived from microarray studies, one limitation has been the lack of standards for presenting and exchanging such data. Here we present a proposal, the Minimum Information About a Microarray Experiment (MIAME), that describes the minimum information required to ensure that microarray data can be easily interpreted and that results derived from its analysis can be independently verified. The ultimate goal of this work is to establish a standard for recording and reporting microarray-based gene expression data, which will in turn facilitate the establishment of databases and public repositories and enable the development of data analysis tools. With respect to MIAME, we concentrate on defining the content and structure of the necessary information rather than the technical format for capturing it.