全文获取类型
收费全文 | 613篇 |
免费 | 11篇 |
国内免费 | 19篇 |
专业分类
系统科学 | 11篇 |
丛书文集 | 2篇 |
教育与普及 | 2篇 |
理论与方法论 | 4篇 |
现状及发展 | 136篇 |
研究方法 | 82篇 |
综合类 | 396篇 |
自然研究 | 10篇 |
出版年
2021年 | 4篇 |
2019年 | 6篇 |
2018年 | 14篇 |
2017年 | 9篇 |
2016年 | 4篇 |
2015年 | 10篇 |
2014年 | 17篇 |
2013年 | 14篇 |
2012年 | 59篇 |
2011年 | 64篇 |
2010年 | 25篇 |
2009年 | 19篇 |
2008年 | 42篇 |
2007年 | 46篇 |
2006年 | 43篇 |
2005年 | 42篇 |
2004年 | 37篇 |
2003年 | 30篇 |
2002年 | 27篇 |
2001年 | 18篇 |
2000年 | 17篇 |
1999年 | 9篇 |
1997年 | 3篇 |
1994年 | 2篇 |
1992年 | 4篇 |
1991年 | 8篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 7篇 |
1971年 | 4篇 |
1970年 | 2篇 |
1969年 | 4篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 7篇 |
1965年 | 3篇 |
排序方式: 共有643条查询结果,搜索用时 15 毫秒
51.
With the rapid growth of e-commerce, customers increasingly write online reviews of the product they purchase. These customer reviews are one of the most valuable sources of information affecting selection of products or services. Summarizing these customer reviews is becoming an interesting area of research, inspiring researchers to develop a more condensed, concise summarization for users. However, most of the current efforts at summarization are based on general product features without feature’s relationship. As a result, these summaries either ignore feedback from customers or do a poor job of reflecting the opinions expressed in customer reviews. To remedy this summarization shortcoming, we propose a feature network-driven quadrant mapping that captures and incorporates opinions from customer reviews. Our focus is on construction of a feature network, which is based on co-occurrence and sematic similarities, and a quadrant display showing the opinions polarity of feature groups. Moreover, the proposed approach involves clustering similar product features, and thus, it is different from standard text summarization based on abstraction and extraction. The summarized results can help customers better understand the overall opinions about a product. 相似文献
52.
Horlings HM Bergamaschi A Nordgard SH Kim YH Han W Noh DY Salari K Joosse SA Reyal F Lingjaerde OC Kristensen VN Børresen-Dale AL Pollack J van de Vijver MJ 《Nature genetics》2008,40(7):809; author reply 810-809; author reply 812
53.
Twigger SN Pruitt KD Fernández-Suárez XM Karolchik D Worley KC Maglott DR Brown G Weinstock G Gibbs RA Kent J Birney E Jacob HJ 《Nature genetics》2008,40(5):523-527
It has been four years since the original publication of the draft sequence of the rat genome. Five groups are now working together to assemble, annotate and release an updated version of the rat genome. As the prevailing model for physiology, complex disease and pharmacological studies, there is an acute need for the rat's genomic resources to keep pace with the rat's prominence in the laboratory. In this commentary, we describe the current status of the rat genome sequence and the plans for its impending 'upgrade'. We then cover the key online resources providing access to the rat genome, including the new SNP views at Ensembl, the RefSeq and Genes databases at the US National Center for Biotechnology Information, Genome Browser at the University of California Santa Cruz and the disease portals for cardiovascular disease and obesity at the Rat Genome Database. 相似文献
54.
Genome sequencing in microfabricated high-density picolitre reactors 总被引:21,自引:0,他引:21
Margulies M Egholm M Altman WE Attiya S Bader JS Bemben LA Berka J Braverman MS Chen YJ Chen Z Dewell SB Du L Fierro JM Gomes XV Godwin BC He W Helgesen S Ho CH Ho CH Irzyk GP Jando SC Alenquer ML Jarvie TP Jirage KB Kim JB Knight JR Lanza JR Leamon JH Lefkowitz SM Lei M Li J Lohman KL Lu H Makhijani VB McDade KE McKenna MP Myers EW Nickerson E Nobile JR Plant R Puc BP Ronan MT Roth GT Sarkis GJ Simons JF Simpson JW Srinivasan M Tartaro KR Tomasz A Vogt KA Volkmer GA Wang SH Wang Y Weiner MP 《Nature》2005,437(7057):376-380
The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments. The apparatus uses a novel fibre-optic slide of individual wells and is able to sequence 25 million bases, at 99% or better accuracy, in one four-hour run. To achieve an approximately 100-fold increase in throughput over current Sanger sequencing technology, we have developed an emulsion method for DNA amplification and an instrument for sequencing by synthesis using a pyrosequencing protocol optimized for solid support and picolitre-scale volumes. Here we show the utility, throughput, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasma genitalium genome with 96% coverage at 99.96% accuracy in one run of the machine. 相似文献
55.
Sakamoto Y Kaneda M Terasaki O Zhao DY Kim JM Stucky G Shin HJ Ryoo R 《Nature》2000,408(6811):449-453
Mesostructured composite materials, with features ranging from 20 to 500 A in size, are obtained by the kinetically controlled competitive assembly of organic and inorganic species into nanostructured domains. Short-range order is limited, and long-range order is determined by weak forces such as van der Waals or hydrogen-bonding. Three-dimensional mesoporous materials obtained by removing the organic phase are of particular interest for applications such as catalysis and chemical sensing or separation, for which structural features such as cavity shape, connectivity and ordered bimodal porosity are critical. But atomic-scale structural characterization by the usual diffraction techniques is challenging for these partially ordered materials because of the difficulty in obtaining large (> 10 microm) single crystals, and because large repeat spacings cause diffraction intensities to fall off rapidly with scattering angle so that only limited small-angle data are available. Here we present a general approach for the direct determination of three-dimensional mesoporous structures by electron microscopy. The structure solutions are obtained uniquely without pre-assumed models or parametrization. We report high-resolution details of cage and pore structures of periodically ordered mesoporous materials, which reveal a highly ordered dual micro- and mesoscale pore structure. 相似文献
56.
Genome shuffling leads to rapid phenotypic improvement in bacteria 总被引:51,自引:0,他引:51
For millennia, selective breeding, on the basis of biparental mating, has led to the successful improvement of plants and animals to meet societal needs. At a molecular level, DNA shuffling mimics, yet accelerates, evolutionary processes, and allows the breeding and improvement of individual genes and subgenomic DNA fragments. We describe here whole-genome shuffling; a process that combines the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes normally associated with conventional breeding. We show that recursive genomic recombination within a population of bacteria can efficiently generate combinatorial libraries of new strains. When applied to a population of phenotypically selected bacteria, many of these new strains show marked improvements in the selected phenotype. We demonstrate the use of this approach through the rapid improvement of tylosin production from Streptomyces fradiae. This approach has the potential to facilitate cell and metabolic engineering and provide a non-recombinant alternative to the rapid production of improved organisms. 相似文献
57.
Charged groups play a critical role in the stability of the helix formed by the isolated C-peptide (residues 1-13 of ribonuclease A) in aqueous solution. One charged-group effect may arise from interactions between charged residues at either end of the helix and the helix dipole. We report here that studies of C-peptide analogues support the helix dipole model, and provide further evidence for the importance of electrostatic interactions not included in the Zimm-Bragg model for alpha-helix formation. 相似文献
58.
讨论了在平面坐标系上摄像机的标定和利用单个摄像机获取三维视觉信息的一种方法。实验结果表明,采用单个摄像机获取三维视觉信息的精度与摄像机相对目标物体的高度及对象物本身的厚度有关,在视场范围内,对象物的厚度越小,摄像机坐标中心相对于目标物体表面中心的距离越远,定位精度越好。 相似文献
59.
Montgomery KT Lee E Miller A Lau S Shim C Decker J Chiu D Emerling S Sekhon M Kim R Lenz J Han J Ioshikhes I Renault B Marondel I Yoon SJ Song K Murty VV Scherer S Yonescu R Kirsch IR Ried T McPherson J Gibbs R Kucherlapati R 《Nature》2001,409(6822):945-946
Our sequence-tagged site-content map of chromosome 12 is now integrated with the whole-genome fingerprinting effort. It provides accurate and nearly complete bacterial clone coverage of chromosome 12. We propose that this integrated mapping protocol serves as a model for constructing physical maps for entire genomes. 相似文献
60.
Choi M Lee SH Kim Y Kang SB Shin J Kwak MH Kang KY Lee YH Park N Min B 《Nature》2011,470(7334):369-373
Controlling the electromagnetic properties of materials, going beyond the limit that is attainable with naturally existing substances, has become a reality with the advent of metamaterials. The range of various structured artificial 'atoms' has promised a vast variety of otherwise unexpected physical phenomena, among which the experimental realization of a negative refractive index has been one of the main foci thus far. Expanding the refractive index into a high positive regime will complete the spectrum of achievable refractive index and provide more design flexibility for transformation optics. Naturally existing transparent materials possess small positive indices of refraction, except for a few semiconductors and insulators, such as lead sulphide or strontium titanate, that exhibit a rather high peak refractive index at mid- and far-infrared frequencies. Previous approaches using metamaterials were not successful in realizing broadband high refractive indices. A broadband high-refractive-index metamaterial structure was theoretically investigated only recently, but the proposed structure does not lend itself to easy implementation. Here we demonstrate that a broadband, extremely high index of refraction can be realized from large-area, free-standing, flexible terahertz metamaterials composed of strongly coupled unit cells. By drastically increasing the effective permittivity through strong capacitive coupling and decreasing the diamagnetic response with a thin metallic structure in the unit cell, a peak refractive index of 38.6 along with a low-frequency quasi-static value of over 20 were experimentally realized for a single-layer terahertz metamaterial, while maintaining low losses. As a natural extension of these single-layer metamaterials, we fabricated quasi-three-dimensional high-refractive-index metamaterials, and obtained a maximum bulk refractive index of 33.2 along with a value of around 8 at the quasi-static limit. 相似文献