网络环境下图书馆数字参考咨询服务的模式与特点

文章对目前网络环境下数字参考咨询服务的几种主要模式与特点，进行了阐述与探讨。     

Structure of astacin and implications for activation of astacins and zinc-ligation of collagenases.

Astacin, a digestive zinc-endopeptidase from the crayfish Astacus astacus L., is the prototype for the 'astacin family', which includes mammalian metallo-endopeptidases and developmentally regulated proteins of man, fruitfly, frog and sea urchin. Here we report the X-ray crystal structure of astacin, which reveals a deep active-site cleft, with the zinc at its bottom ligated by three histidines, a water molecule and a more remote tyrosine. The third histidine (His 102) forms part of a consensus sequence, shared not only by the members of the astacin family, but also by otherwise sequentially unrelated proteinases, such as vertebrate collagenases. It may therefore represent the elusive 'third' zinc ligand in these enzymes. The amino terminus of astacin is buried forming an internal salt-bridge with Glu 103, adjacent to His 102. Astacin pro-forms extended at the N terminus, as observed for some 'latent' mammalian astacin homologues, did not exhibit this 'active' conformation, indicating an activation mechanism reminiscent of trypsin-like serine proteinases.     

Exocytotic fusion is activated by Rab3a peptides.

Studies of intracellular traffic in yeast and mammalian systems have implicated members of the Rab family of small GTP-binding proteins as regulators of membrane fusion. We have used the patch clamp technique to measure exocytotic fusion events directly and investigate the role of GTP-binding proteins in regulating exocytosis in mast cells. Intracellular perfusion of mast cells with GTP-gamma S is sufficient to trigger complete exocytotic degranulation in the absence of other intracellular messengers. Here we show that GTP is a potent inhibitor of GTP-gamma S-induced degranulation, indicating that sustained activation of a GTP-binding protein is sufficient for membrane fusion. We have found that synthetic oligopeptides, corresponding to part of the effector domain of Rab3a, stimulate complete exocytotic degranulation, similar to that induced by GTP-gamma S. The response is selective for Rab3a sequence and is strictly dependent on Mg2+ and ATP. This suggests that sustained activation of a Rab3 protein causes exocytotic fusion. The peptide response can be accelerated by GDP-beta S, suggesting that Rab3a peptides compete with endogenous Rab3 proteins for a binding site on a target effector protein, which causes fusion on activation.     

矛盾的根本属性与对外开放

矛盾根本属性原理是我们做好的对外开放工作的指导思想。在对外开放过程中，社会主义中国同国际资本主义双方的矛盾同一性是占据主导地位的，同时也必然存在着矛盾斗争性，但这是第二位的。我们应正确处理对外开放中矛盾同一性与斗争性的关系，着眼于矛盾双方的同一性，使必要的斗争性服务与服从于同一性，以利于对外开放基本国策的实施，加速中国社会主义现代化建设的进程。     

Which survey indicators are useful for monitoring consumption? Evidence from European countries

This paper assesses the information content of two survey indicators for consumption developments in the near future for eight European countries in the period 1985–1998. Empirical work on this topic typically focuses on consumer confidence, the perceptions of buyers of consumption goods. This paper examines whether perceptions of sellers of consumption goods, measured by retail trade surveys, may also improve short‐term monitoring of consumption. We find that both consumer confidence and retailer confidence embody valuable information, when analysed in isolation. For France, Italy and Spain we conclude that adding retail confidence does not improve the indicator model once consumer confidence has been included. For the UK the reverse case is obtained. For the remaining four countries we show that combining consumer sentiment and retail trade confidence into a composite indicator leads to optimal results. Our results suggest that incorporating information from retail trade surveys may offer significant benefits for the analysis of short‐term prospects of consumption. Copyright © 2004 John Wiley & Sons, Ltd.     

Environmental Issues of Geothermal Development in Yangbajing, Tibet and the Countermeasures

The pollution source and pollution state of surface water is respectively assessed with equal-standard-pollution-load index and Nemero index, and the habitans' health is assessed as well by comparison with pre-development of geothermal field. Results show that: 1 ) Three pollutive factors obviously fail to attain the required standards and their order sorted by impact degree is As＞F- ＞S2-. 2) The Nemero index of Donggua cross section of Duilongqu River is 0. 725and the water quality is at mildly-polluted level. 3) In Lhasa River, the Nemero indexes of cross sections affected by geothermal fields are much higher than that of the unaffected one, which shows the present and potential impacts of geothermal development. 4) Much more inhabitants nearby got trichomadesis sickness after geothermal development, which shows that the surface water pollution in this area has threatened the inhabitants' health. In addition, other environmental issues caused by Yangbajing geothermal development are analyzed and some countermeasures are proposed.