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961.
Recent studies of n-type semiconductors have demonstrated spin-coherent transport over macroscopic distances, with spin-coherence times exceeding 100 ns; such materials are therefore potentially useful building blocks for spin-polarized electronics ('spintronics'). Spin injection into a semiconductor (a necessary step for spin electronics) has proved difficult; the only successful approach involves classical injection of spins from magnetic semiconductors. Other work has shown that optical excitation can provide a short (<500 ps) non-equilibrium burst of coherent spin transfer across a GaAs/ZnSe interface, but less than 10% of the total spin crosses into the ZnSe layer, leaving long-lived spins trapped in the GaAs layer (ref. 9). Here we report a 'persistent' spin-conduction mode in biased semiconductor heterostructures, in which the sourcing of coherent spin transfer lasts at least 1-2 orders of magnitude longer than in unbiased structures. We use time-resolved Kerr spectroscopy to distinguish several parallel channels of interlayer spin-coherent injection. The relative increase in spin-coherent injection is up to 500% in the biased structures, and up to 4,000% when p-n junctions are used to impose a built-in bias. These experiments reveal promising opportunities for multifunctional spin electronic devices (such as spin transistors that combine memory and logic functions), in which the amplitude and phase of the net spin current are controlled by either electrical or magnetic fields. 相似文献
962.
Mutations in PTPN11, encoding the protein tyrosine phosphatase SHP-2, cause Noonan syndrome. 总被引:23,自引:0,他引:23
M Tartaglia E L Mehler R Goldberg G Zampino H G Brunner H Kremer I van der Burgt A H Crosby A Ion S Jeffery K Kalidas M A Patton R S Kucherlapati B D Gelb 《Nature genetics》2001,29(4):465-468
Noonan syndrome (MIM 163950) is an autosomal dominant disorder characterized by dysmorphic facial features, proportionate short stature and heart disease (most commonly pulmonic stenosis and hypertrophic cardiomyopathy). Webbed neck, chest deformity, cryptorchidism, mental retardation and bleeding diatheses also are frequently associated with this disease. This syndrome is relatively common, with an estimated incidence of 1 in 1,000-2,500 live births. It has been mapped to a 5-cM region (NS1) [corrected] on chromosome 12q24.1, and genetic heterogeneity has also been documented. Here we show that missense mutations in PTPN11 (MIM 176876)-a gene encoding the nonreceptor protein tyrosine phosphatase SHP-2, which contains two Src homology 2 (SH2) domains-cause Noonan syndrome and account for more than 50% of the cases that we examined. All PTPN11 missense mutations cluster in interacting portions of the amino N-SH2 domain and the phosphotyrosine phosphatase domains, which are involved in switching the protein between its inactive and active conformations. An energetics-based structural analysis of two N-SH2 mutants indicates that in these mutants there may be a significant shift of the equilibrium favoring the active conformation. This implies that they are gain-of-function changes and that the pathogenesis of Noonan syndrome arises from excessive SHP-2 activity. 相似文献
963.
A defined range of guard cell calcium oscillation parameters encodes stomatal movements. 总被引:59,自引:0,他引:59
G J Allen S P Chu C L Harrington K Schumacher T Hoffmann Y Y Tang E Grill J I Schroeder 《Nature》2001,411(6841):1053-1057
Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environmental and endogenous signals to regulate the aperture of stomatal pores and [Ca2+]cyt oscillations are a fundamental component of stomatal closure. Here we systematically vary [Ca2+]cyt oscillation parameters in Arabidopsis guard cells using a 'calcium clamp' and show that [Ca2+]cyt controls stomatal closure by two mechanisms. Short-term 'calcium-reactive' closure occurred rapidly when [Ca2+]cyt was elevated, whereas the degree of long-term steady-state closure was 'calcium programmed' by [Ca2+]cyt oscillations within a defined range of frequency, transient number, duration and amplitude. Furthermore, in guard cells of the gca2 mutant, [Ca2+]cyt oscillations induced by abscisic acid and extracellular calcium had increased frequencies and reduced transient duration, and steady-state stomatal closure was abolished. Experimentally imposing [Ca2+]cyt oscillations with parameters that elicited closure in the wild type restored long-term closure in gca2 stomata. These data show that a defined window of guard cell [Ca2+]cyt oscillation parameters programs changes in steady-state stomatal aperture. 相似文献
964.
When acrylic dope ( polyacrylonitrile-sodium thiocya-nate aqueous solution system) is extruded from a capillary, the fluctuation phenomenon in the extrusion pressure is discovered. The influences of extrusion shear rate, temperature and ratio of length to diameter of capillary on this phenomenon are investigated. It is shown that the dope sample recovers its elastic deformation partially during the course of its flowing through the capillary , and this part of elasticity energy is dissipated in the form of the heat produced by viscous resistance. And the heat in turn makes changes in the sample's elasticity, finally leads to the occurrence of pressure' s fluctuation. 相似文献
965.
966.
Crnković-Mertens I Wagener N Semzow J Gröne EF Haferkamp A Hohenfellner M Butz K Hoppe-Seyler F 《Cellular and molecular life sciences : CMLS》2007,64(9):1137-1144
Cancer cells are typically characterized by apoptosis deficiency. In order to investigate a possible role for the anti-apoptotic
livin gene in renal cell cancer (RCC), we analyzed its expression in tumor tissue samples and in RCC-derived cell lines. In addition,
we studied the contribution of livin to the apoptotic resistance of RCC cells by RNA interference (RNAi). Livin gene expression was detected in a significant portion of RCC tumor tissue specimens (13/14, 92.9%) and tumor-derived cell
lines (12/15, 80.0%). Moreover, targeted inhibition of livin by RNAi markedly sensitized RCC cells towards proapoptotic stimuli, such as UV irradiation or the chemotherapeutic drugs
etoposide, 5-fluorouracil, and vinblastine. These effects were specific for livin expressing tumor cells. We conclude that livin can contribute significantly to the apoptosis resistance of RCC cells. Targeted inhibition of livin could represent a novel therapeutic strategy to increase the sensitivity of renal cancers towards pro-apoptotic agents.
Received 30 November 2006; received after revision 22 February 2007; accepted 20 March 2007 相似文献
967.
Alzheimer disease (AD), while chronic and progressive with an average progression of 7 – 10 years, is both multifactorial
and heterogeneous. Thus, AD offers a large window of opportunity and a large number of therapeutic targets to inhibit it.
The selection of a therapeutic target, however, is one of the biggest challenges in developing a pharmacological treatment
of this multifactorial disease. Inhibition of a pivotal downstream event is likely to benefit more patients than inhibition
of an upstream event in AD pathogenesis. Neurofibrillary degeneration of abnormally hyperphosphorylated tau offers such a
pivotal therapeutic target. Abnormal hyperphosphorylation of tau and not its aggregation into filaments appears to be the
most deleterious step in neurofibrillary degeneration. Tau can be abnormally hyperphosphorylated by downregulation of protein
phosphatase-2A activity or by upregulation of more than one tau kinase. Restoration of the phosphatase activity which is downregulated
in AD brain or inhibition of GSK-3β and cdk5, which are required for AD-type abnormal hyperphosphorylation of tau, are among
the most promising therapeutic strategies. 相似文献
968.
The RecQ family of DNA helicases is highly conserved throughout evolution and plays an important role in the maintenance of
genomic stability in all organisms. Mutations in three of the five known family members in humans, BLM, WRN and RECQL4, give rise to disorders that are characterized by predisposition to cancer and premature aging, emphasizing the importance
of studying the RecQ proteins and their cellular activities. Interestingly, three autosomal recessive disorders have been
associated with mutations in the RECQL4 gene: Rothmund-Thomson, RAPADILINO, and Baller-Gerold syndromes, thus making RECQL4 unique within the RecQ family of DNA
helicases. To date, however, the molecular function of RECQL4 and the possible cellular pathways in which it is involved remain
poorly understood. Here, we present an overview of recent findings in connection with RECQL4 and try to highlight different
directions the field could head, helping to clarify the role of RECQL4 in preventing tumorigenesis and maintenance of genome
integrity in humans.
Received 31 October 2006; received after revision 4 January 2007; accepted 5 February 2007 相似文献
969.
The computational prediction of gene and protein function is rapidly gaining ground as a central undertaking in computational biology. Making sense of the flood of genomic data requires fast and reliable annotation. Many ingenious algorithms have been devised to infer a protein's function from its amino acid sequence, 3D structure and chromosomal location of the encoding genes. However, there are significant challenges in assessing how well these programs perform. In this article we explore those challenges and review our own attempt at assessing the performance of those programs. We conclude that the task is far from complete and that a critical assessment of the performance of function prediction programs is necessary to make true progress in computational function prediction. 相似文献
970.
Ras GTPases mediate a wide variety of cellular processes by converting a multitude of extracellular stimuli into specific biological responses including proliferation, differentiation and survival. In mammalian cells, three ras genes encode four Ras isoforms (H-Ras, K-Ras4A, K-Ras4B and N-Ras) that are highly homologous but functionally distinct. Differences between the isoforms, including their post-translational modifications and intracellular sorting, mean that Ras has emerged as an important model system of compartmentalised signalling and membrane biology. Ras isoforms in different subcellular locations are proposed to recruit distinct upstream and downstream accessory proteins and activate multiple signalling pathways. Here, we summarise data relating to isoform-specific signalling, its role in disease and the mechanisms promoting compartmentalised signalling. Further understanding of this field will reveal the role of Ras signalling in development, cellular homeostasis and cancer and may suggest new therapeutic approaches. 相似文献