化学发光自显影免疫印迹法（Western Blotting）检测绒山羊皮肤组织中催乳素受体蛋白

采集不同季节绒山羊皮肤组织样品,提取总蛋白,经聚丙烯酰胺凝胶电泳,将电泳结果转移到醋酸纤维薄膜,然后与催乳素抗体结合、辣根过氧化物酶标记二抗结合,后者作用于化学发光底物,暗室中置X-光胶片上自显影数分钟,经显影、定影,得到黑色曝光斑,根据胶片上曝光斑的位置确定出催乳素受体的分子量为87kD。根据不同时期样品黑斑的大小得出各时期催乳素受体表达量与绒毛生长趋势是一致的。     

Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry

A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme EcoRI and cloned into the phage lambda gtWES.lambda B. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified.     

道路交通驾驶员差异性分类定量研究

驾驶员个体差异是影响城市道路交通运营、效率及安全的一个重要因素之一。为了实现对驾驶员差异性的影响分析,采用驾驶模拟舱研究平台对驾驶员样本进行归类。研究分别选取了反映驾驶员特征的静态驾驶适性和动态驾驶适性两类,共计15个指标。采用因子分析的方法将相关指标归纳为驾驶员的5个类因子,并通过主成份分析的方法找出每类因子中的主导指标,并以此指标作为聚类分析的输入变量。结果表明聚类分析得到的三类驾驶员具有明显的各自特征。     

Evolution and maintenance of cooperation via inheritance of neighborhood relationship

Cooperative behaviors are ubiquitous in nature and human society. It is very important to understand the internal mechanism of emergence and maintenance of cooperation. As we know now, the offsprings inherit not only the phenotype but also the neighborhood relationship of their parents. Some recent research results show that the interactions among individuals facilitate survival of cooperation through network reciprocity of clustering cooperators. This paper aims at introducing an inheritance mechanism of neighborhood relationship to explore the evolution of cooperation. In detail, a mathematical model is proposed to characterize the evolutionary process with the above inheritance mechanism. Theoretical analysis and numerical simulations indicate that high-level cooperation can emerge and be maintained for a wide variety of cost-to-benefit ratios, even if mutation happens during the evolving process.     

Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1

Spinocerebellar ataxia type 1 (SCA1) is a dominantly inherited neurodegenerative disease caused by expansion of a glutamine-encoding repeat in ataxin 1 (ATXN1). In all known polyglutamine diseases, the glutamine expansion confers toxic functions onto the protein; however, the mechanism by which this occurs remains enigmatic, in light of the fact that the mutant protein apparently maintains interactions with its usual partners. Here we show that the expanded polyglutamine tract differentially affects the function of the host protein in the context of different endogenous protein complexes. Polyglutamine expansion in ATXN1 favours the formation of a particular protein complex containing RBM17, contributing to SCA1 neuropathology by means of a gain-of-function mechanism. Concomitantly, polyglutamine expansion attenuates the formation and function of another protein complex containing ATXN1 and capicua, contributing to SCA1 through a partial loss-of-function mechanism. This model provides mechanistic insight into the molecular pathogenesis of SCA1 as well as other polyglutamine diseases.     

播种方式对紫花苜蓿＋无芒雀麦人工草地土壤剖面养分分布的影响

采用田间调查取样和室内分析相结合的方法,2007年在内蒙古民族大学农学院试验农场以2年生单播紫花苜蓿、单播无芒雀麦、隔行混播和同行混播人工草地为研究对象,采用分层取样法研究了不同播种方式对土壤理化性质的影响,结果表明：在牧草生长期间单播无芒雀麦草地土壤容重最小,紫花苜蓿草地土壤容重最大;单播无芒雀麦、隔行混播和同行混播草地0～20cm土层有机质含量大于单播紫花苜蓿草地,20～30cm不显著,30～40cm单播紫花苜蓿草地显著增加,分别是隔行混播草地、同行混播和单播无芒雀麦草地的1.11、1.72和1.94倍;速效N单播无芒雀麦草地最低,速效P、速效K单播紫花苜蓿草地最低.     

基于CVaR的能源互补联合系统优化配置模型研究

由于风电、光电出力具有不确定性,因此会引起电网频率的较大波动,严重影响电能质量.为了保证含有新能源电力系统的稳定运行,特提出风光水储联合调度优化模型.首先,建立含新能源的电力系统优化调度数学模型,该模型追求系统机组耗量最小.在进行含新能源发电的电力系统优化调度中,可被用来调度的出力实际为满足负荷功率后,扣除新能源输出功率的那部分火电机组出力.算例结果表明,本文采取CVaR具有良好的性能;风电与光伏清洁能源机组并入系统中增加了日前调度的不确定性风险,但通过CVaR评估计算系统风险,采取有效的措施会使系统发电成本减少与碳排放量减少.