Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

再辉效应对T9A钢显微组织和机械性能的影响

研究了T9A钢铅浴淬火时,再辉效应对其显微组织和机械性能的影响.在珠光体转变温度范围内等温时,随着等温温度的降低,引起塑性下降的原因是再辉效应促发颗粒状或短片状珠光体形成;在上贝氏体转变温度区,由于出现再辉效应产生的温度爬升现象,使试样温度升至屈氏体转变温度区从而形成上贝氏体和屈氏体的混合组织,使得强度增高曲线出现平台或稍有下降,且塑性发生下降,同时,还对等温淬火的定义提出了新的看法,绘出了反映实际情况的共析钢C-曲线上的组织形成图。     

骨髓干细胞的可塑性及其在栓塞性疾病中的应用

栓塞性血管阻塞在急性心肌梗死、脑梗塞、血栓闭塞性脉管炎、糖尿病足等发病过程中起着重要作用。目前临床上针对这些疾病的治疗手段主要有：药物溶栓、血管旁路移植和介入手术等,多年来其治疗方法无实质性进展。上个世纪九十年代人们发现了干细胞新的生物学特性—可塑性,对干细胞可塑性的研究以及相关技术的发展为心脏病、老年痴呆、帕金森等的治疗带来了希望,也为栓塞性疾病的治疗开辟了新途径,有望成为治疗手段之一。     

中晚期高危妊娠引产32例分析及经验总结

目的：分析中晚期妊娠合并症引产病例的特征，适当引产方式的选择。总结引产处理成功的经验。方法：总结32例中晚期妊娠合并内外科疾病选择引产病例，分析各病种分布以及引产方式、引产并发症。结果：32例病例中肝肾疾病占31％，选择利凡诺引产15例，水囊引产12例，药物引产4例。均无内外科并发症发生。结论：做好有妊娠合并症的中晚期引产的术前术中准备工作，选择合适的引产方式是引产成功安全的关键。     

非有效市场条件下证券交易税实证研究

主要分析在股市存在泡沫的情况下,证券交易税税率提高对采取正反馈投资策略的噪音交易者和理性投资的被动交易者及套利者的影响,在理论不支持征收交易税降低市场波动的宏观调节政策.