全文获取类型
收费全文 | 341篇 |
免费 | 0篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 9篇 |
现状及发展 | 37篇 |
研究方法 | 74篇 |
综合类 | 205篇 |
自然研究 | 17篇 |
出版年
2021年 | 1篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 3篇 |
2015年 | 2篇 |
2014年 | 1篇 |
2013年 | 4篇 |
2012年 | 30篇 |
2011年 | 58篇 |
2010年 | 3篇 |
2008年 | 29篇 |
2007年 | 20篇 |
2006年 | 35篇 |
2005年 | 38篇 |
2004年 | 22篇 |
2003年 | 21篇 |
2002年 | 26篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1981年 | 1篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1961年 | 1篇 |
排序方式: 共有342条查询结果,搜索用时 15 毫秒
341.
A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity 总被引:1,自引:0,他引:1
Many bacterial pathogens of plants and animals use a type III secretion system to deliver diverse virulence-associated 'effector' proteins into the host cell. The mechanisms by which these effectors act are mostly unknown; however, they often promote disease by suppressing host immunity. One type III effector, AvrPtoB, expressed by the plant pathogen Pseudomonas syringae pv. tomato, has a carboxy-terminal domain that is an E3 ubiquitin ligase. Deletion of this domain allows an amino-terminal region of AvrPtoB (AvrPtoB(1-387)) to be detected by certain tomato varieties leading to immunity-associated programmed cell death. Here we show that a host kinase, Fen, physically interacts with AvrPtoB(1-387 )and is responsible for activating the plant immune response. The AvrPtoB E3 ligase specifically ubiquitinates Fen and promotes its degradation in a proteasome-dependent manner. This degradation leads to disease susceptibility in Fen-expressing tomato lines. Various wild species of tomato were found to exhibit immunity in response to AvrPtoB(1-387 )and not to full-length AvrPtoB. Thus, by acquiring an E3 ligase domain, AvrPtoB has thwarted a highly conserved host resistance mechanism. 相似文献
342.
Stream denitrification across biomes and its response to anthropogenic nitrate loading 总被引:11,自引:0,他引:11
Mulholland PJ Helton AM Poole GC Hall RO Hamilton SK Peterson BJ Tank JL Ashkenas LR Cooper LW Dahm CN Dodds WK Findlay SE Gregory SV Grimm NB Johnson SL McDowell WH Meyer JL Valett HM Webster JR Arango CP Beaulieu JJ Bernot MJ Burgin AJ Crenshaw CL Johnson LT Niederlehner BR O'Brien JM Potter JD Sheibley RW Sobota DJ Thomas SM 《Nature》2008,452(7184):202-205
Anthropogenic addition of bioavailable nitrogen to the biosphere is increasing and terrestrial ecosystems are becoming increasingly nitrogen-saturated, causing more bioavailable nitrogen to enter groundwater and surface waters. Large-scale nitrogen budgets show that an average of about 20-25 per cent of the nitrogen added to the biosphere is exported from rivers to the ocean or inland basins, indicating that substantial sinks for nitrogen must exist in the landscape. Streams and rivers may themselves be important sinks for bioavailable nitrogen owing to their hydrological connections with terrestrial systems, high rates of biological activity, and streambed sediment environments that favour microbial denitrification. Here we present data from nitrogen stable isotope tracer experiments across 72 streams and 8 regions representing several biomes. We show that total biotic uptake and denitrification of nitrate increase with stream nitrate concentration, but that the efficiency of biotic uptake and denitrification declines as concentration increases, reducing the proportion of in-stream nitrate that is removed from transport. Our data suggest that the total uptake of nitrate is related to ecosystem photosynthesis and that denitrification is related to ecosystem respiration. In addition, we use a stream network model to demonstrate that excess nitrate in streams elicits a disproportionate increase in the fraction of nitrate that is exported to receiving waters and reduces the relative role of small versus large streams as nitrate sinks. 相似文献