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931.
Chao Gu YongRui Du Yan Gao Zhi Yao Xin Gu QiuYue Zhang JingJing Xu WeiMin Deng 《科学通报(英文版)》2012,57(11):1288-1297
There is accumulating evidence that cancer stem cells (CSCs) play an important role in tumor progression. Novel strategies targeting CSCs have been widely researched. In the present study, we explored whether such CSCs existed in human ovarian cancer (OVCA) cell line and whether anti-CD44 antibody had effects on such subpopulation. We isolated and identified spheroid cells from SKOV-3. Then we used A3D8, an anti-CD44 mAb to treat spheroid cells with so-called stemness. Effects of A3D8 on spheroid cells’ biological behaviors were examined. Our findings showed that there was a small subpopulation that had so-called stemness in SKOV-3 cell line. Against spheroid cells, A3D8 can (1) inhibit cell proliferation; (2) change cell cycle distribution and expression of p21, CDK2 and cyclinA; (3) enhance cisplatin (DDP)-induced apoptosis; (4) promote cell differentiation; (5) inhibit clone formation efficiency; (6) reduce invasive efficacy; (7) inhibit tumorigenicity. Thus, to sum up points which we have just showed, spheroid cells isolated from SKOV-3 can be used as an appropriate in vitro model for relevant study of human ovarian CSCs. And our results reasoned that anti-CD44 therapy may become a potential promising strategy for OVCA treatment. 相似文献
932.
The mechanism of oxidative damage to deoxyribonucleic acid (DNA) by iron-containing mesoporous molecular sieves (MCM-41) irradiated with visible light was elucidated. Fe-loaded MCM-41 (Fe/MCM-41) was used as a photocatalyst and the damage to calf thymus DNA caused by hydrogen peroxide (H2O2) was studied. The damage and extent of oxidation of DNA were measured by high-performance liquid chromatography (HPLC) and intermediate products were detected by HPLC/electrospray ionization tandem mass spectrometry. Electron spin resonance was used to detect changes in reactive oxygen species and peroxidase catalytic spectrophotometry was used to determine the concentration of H2O2. The results indicated that Fe/MCM-41 efficiently activated H2O2 in solution at pH 4.0-8.0 under irradiation with visible light. The photocatalytic system degraded DNA most effectively at pH 5.0-6.0 but also operated at pH 8.0. At pH 4.2, the degree of DNA damage reached 25.65% after 5 h and the kinetic constant was 5.89×10 2 min 1. Damage to DNA was predominantly caused by hydroxyl radicals generated in the system. The mechanism of DNA damage is of potential concern to human health because it can occur in neutral solutions irradiated by visible light. 相似文献
933.
934.
本文从实用角度讨论了MCS-51系列单片机几条指令的实质,指出对汇编语言指令的理解与应用不能只停留在指令字面意义的简单表达上,而应该了解指令的详细执行过程,掌握其实质. 相似文献
935.
报道了一种用纸色谱选择展开剂范围,薄层色谱确定展开剂,干层柱层析法提纯高分子显色剂的新方法.通过薄层色谱,元素分析及红外光谱测定证明,提纯的结果令人满意 相似文献
936.
叙述人工神经元调节器的结构和原理,给出联接权训练算法的实现方法,设计人工神经元作调节器的双闭环直流调速系统的动态结构,并进行系统仿真研究。 相似文献
937.
用Excel电子表格软件对一元和二元函数的物性数据进行模拟。模拟用的方程来自原始公式、化工热力学、物理化学或经验模型,在多数情况下计算值和实验值之间的误差小于1%,从而为化工计算提供了新的实用手段。 相似文献
938.
对桑塔纳330K8BLOL型轿车以1.9LTDI柴油机为配套动力时,从动力性和经济性角度对传动系统的参数进行了优化,提出了合适的变速器传动比和主传动比.使用SantanaLX五档变速器,且主传动比为3.889,可使轿车动力性有所提高,并且使油耗减少30%左右. 相似文献
939.
细茎大豆(G.gracilis)rbcS基因结构与分子进化分析 总被引:4,自引:0,他引:4
从细茎大豆的嫩叶中提取总DNA,用PCR方法扩增得到包含完整编码区的rbcS基因并将其克隆到pBLUESCRIPT载体中。完成全基因1089个核苷酸测序后,运用PCGENE进行顺序编辑和同源比较,并应用MEGA1.021软件中的Neighbor-joining方面画出Rubisco小亚基仓的系统进化树。 相似文献
940.
This work reported on the surface modification of high strength polyethylene(HSPE)fiber by chemical etching in chromic acid.The surface chemistry,the functional group contents, the wetting ability, the surface morphology and the tensile strength of modified fibers were investigated. The results show that, the surface roughness,surface wet properties and active functional group can be introduced on the HSPE fiber surface by this method,which will improve the interface adhesion in the composites, and the decrease of the tensile strength of modified fibers can be controlled. 相似文献