Cloning of the hepatitis B virus genome in Escherichia coli]

The whole genome of the hepatitis B virus (Dane particles) was inserted in vitro in the genome of the bacteriophage lambda gtWES . LAMBDA B. The recombinant DNA molecule was cloned in E. coli. Amplification of the hybrid bacteriophage enables the preparation of large amounts of hepatitis B virus DNA. The possibilities offered by the utilization of this recombinant bacteriophage are discussed.     

Synthesis of N-aryl-N-glycolyl hydroxamic acids: Nonmicrosomal metabolites of nitrosoaromatics

Summary A method for the synthesis of N-aryl-N-glycolyl hydroxamic acids is described. This method consists of N-acylation of an arylhydroxylamine by glycolic acid in the presence of dicyclohexylcarbodiimide.This study was supported by grant No. CA 21668 from the National Cancer Institute, DHEW.     

Effect of hormones on adenyl cyclase activity of rabbit mammary gland in vitro

Ovarian hormones namely beta-estradiol and progesterone were observed to stimulate the activity of adenyl cyclase of the mammary gland from pregnant rabbit in vitro, unlike the lactating tissue where it was inhibited. On the other hand, non-ovarian hormones like hydrocortisone, prolactin and insulin did not have a similar effect on this enzyme.