Comparative studies on phenotypic plasticity of two herbs, Changium smyrnioides and Anthriscus sylvestris

The endangered medicinal herb, Changium smyrnioides can only be found in deciduous forest gaps within the middle to northern subtropical broad-leaved evergreen forest zone of China. The considerable plasticity of its shoot and root structure helps it to capture light more effectively in winter and early spring, and to adapt to the soil moisture conditions in its narrow habitat. Another medicinal plant, Anthriscus sylvestris, is of similar economic importance but commonly distributed widely. In contrast to C. smyrnioides, it has low structural plasticity. It is also specialized to adapt to the moist and sunny environment, where habitat, such as the banks of creeks and rivers, is abundant.     

Chromosome cohesion is regulated by a clock gene paralogue TIM-1

Faithful transmission of the genome requires that a protein complex called cohesin establishes and maintains the regulated linkage between replicated chromosomes before their segregation. Here we report the unforeseen participation of Caenorhabditis elegans TIM-1, a paralogue of the Drosophila clock protein TIMELESS, in the regulation of chromosome cohesion. Our biochemical experiments defined the C. elegans cohesin complex and revealed its physical association with TIM-1. Functional relevance of the interaction was demonstrated by aberrant mitotic chromosome behaviour, embryonic lethality and defective meiotic chromosome cohesion caused by the disruption of either TIM-1 or cohesin. TIM-1 depletion prevented the assembly of non-SMC (structural maintenance of chromosome) cohesin subunits onto meiotic chromosomes; however, unexpectedly, a partial cohesin complex composed of SMC components still loaded. Further disruption of cohesin activity in meiosis by the simultaneous depletion of TIM-1 and an SMC subunit decreased homologous chromosome pairing before synapsis, revealing a new role for cohesin in metazoans. On the basis of comparisons between TIMELESS homologues in worms, flies and mice, we propose that chromosome cohesion, rather than circadian clock regulation, is the ancient and conserved function for TIMELESS-like proteins.     

d(f)=i的平面图

Ｓａｃｈｓ,Ｋｏｚｙｓｅｖ和Ｇｒｉｈｂｅｒｙ指出平面图具有Ｈａｍｉｌｔｏｎ图的一个必要条件是ｎｉ＝３（ｉ－２）ｉ＝ｎｉ＝３（ｉ－２）′ｉ＝ｎ－２,其中ｉ和′ｉ分别为Ｈａｍｉｌｔｏｎ圈内、外度为ｉ的面数。本文探讨面的度相等的平面图及面并成顶点在边界上的连通区域的面数．     

T7启动子促发下的人尿激酶原在大肠杆菌中的高效表达

将人工全化学合成的尿激酶原（ｐｒｏ－ｕｒｏｋｉｎａｓｅ）ｃＤＮＡ克隆到表达载体ｐＥＴ３ｄ中，转化大肠杆菌ＢＬ２１（ＤＥ３）ＬｙｓＳ，经ＩＰＴＧ诱导，获得了占菌体总蛋白１８％的高表达。经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎ－ｂｌｏｔ鉴定，表达产物主要为单链尿激酶，并且在菌体内基本以无活性的包涵体形式存在。经体外变复性，从ＩＬ培养基中可获得３０００００单位的活性尿激酶原。     

锥形血管中的血液流动

本文研究了带有锥度的血管的发展流动问题,导得了一组相应的速度分布、压力分布公式。分析表明,锥度角对血管流场是有影响的。当略去锥度角时,本文公式便变为和无锥度角的等圆截面直管的Targ公式相一致。     

An estimation of ozone flux in a stratosphere-troposphere exchange event

A new method based on mass fluxes and observed ozone profiles was developed to estimate crosstropopause ozone flux. Using this method, we estimated the cross-tropopause ozone flux in a stratospheric-tropospheric exchange event that occurred over East Asia in March 2001.The result revealed that the ozone flux across the tropopause in this event was an order of magnitude higher than the global and hemispheric average. Compared to the traditional method using a linear relationship between ozone mixing ratio and potential vorticity near the tropopause, the cross-tropopause ozone flux evaluated with ozonesonde data was somewhat higher, although the orders of the two values were the same.     

Effects of several preoperative medications on fat cell lipolysis,and activity of adipose tissue cyclic AMP phosphodiesterase

Summary Effects were examined of atropine, diazepam, pethidene, promethazine, scopolamine, omnopon and papaverine on basal and noradrenaline-stimulated lipolysis in rat isolated fat cells and on rat adipose tissue cyclic AMP phosphodiesterase activity. Papaverine at high concentration (1 mM) inhibited both basal and hormone-stimulated lipolysis, whereas diazepam enhanced basal lipolysis. At a clinical dose, omnopon increased both basal and noradrenaline-stimulated lipolysis. Adipose tissue cAMP phosphodiesterase activity was strongly inhibited by 1 mM diazepam, papaverine, promethazine and omnopon (280 g ml^–1). Lack of enhancement of lipolysis by the established cAMP phosphodiesterase antagonist papaverine, is compartible with simultaneous inhibition also of adipose adenyl cyclase. Diazepam-stimulated lipolysis is compatible with its phosphodiesterase inhibitory activity. It is proposed that papaverine-containing omnopon may offer some survival advantages during surgical stress by facilitating a caloric supply.The authours are grateful to Dr D. C. Williams for his continued support and encouragement.     

热水浸提法提取库拉索芦荟花多糖的研究

采用热水浸提法提取库拉索芦荟花多糖,对温度、时间、加水量、芦荟花粒度4个工艺条件进行了研究.本实验条件下,芦荟花多糖提取的最佳条件为:温度:100℃;粒度:40-60目;时间:4 h;加水量:100 mL.分两次提取比单次提取多糖收率可提高10%.     

瞬态诱发耳声发射信号近似熵的分析及应用

为研究有无对侧刺激声 (CAS)作用下 ,瞬态诱发耳声发射 (TEOAE)信号的变化特征 ,根据耳蜗的非线性特性 ,提出了将近似熵的分析方法引入到耳声发射信号的研究中 ,并将近似熵的分析方法从时间序列复杂性的度量中引入到频率序列复杂性的度量中。结果表明 :无 CAS作用下 ,TEOAE频域信号的近似熵在各频带处 ,蜗性病变耳的近似熵明显低于正常耳的近似熵 ,蜗后病变耳的近似熵与正常耳的近似熵无明显差别。在 CAS作用下 ,听力正常耳的近似熵下降 ,而感音神经性耳聋的近似熵上升 ,且近似熵上升的频带与耳蜗或蜗后神经患病处频带相关 .根据研究结果 ,提出了利用有无对侧刺激声作用下耳声发射信号近似熵的变化来诊断感音神经性耳聋的方法