The constitution of filixic acid

Zusammenfassung Es wird die Vermutung ausgesprochen, dass Filixsäure, ein Inhaltsstoff von Farnwurzeln, die Konstitutionsformel (V) hat.     

Restoration of type VII collagen expression and function in dystrophic epidermolysis bullosa

Dystrophic epidermolysis bullosa (DEB) is a family of inherited mechano-bullous disorders caused by mutations in the human type VII collagen gene (COL7A1). Individuals with DEB lack type VII collagen and anchoring fibrils, structures that attach epidermis and dermis. The current lack of treatment for DEB is an impetus to develop gene therapy strategies that efficiently transfer and stably express genes delivered to skin cells in vivo. In this study, we delivered and expressed full-length type VII collagen using a self-inactivating minimal lentivirus-based vector. Transduction of lentiviral vectors containing the COL7A1 transgene into recessive DEB (RDEB) keratinocytes and fibroblasts (in which type VII collagen was absent) resulted in persistent synthesis and secretion of type VII collagen. Unlike RDEB parent cells, the gene-corrected cells had normal morphology, proliferative potential, matrix attachment and motility. We used these gene-corrected cells to regenerate human skin on immune-deficient mice. Human skin regenerated by gene-corrected RDEB cells had restored expression of type VII collagen and formation of anchoring fibrils at the dermal-epidermal junction in vivo. These studies demonstrate that it is possible to restore type VII collagen gene expression in RDEB skin in vivo.     

用多因素方差分析法研究光谱标样的均匀性

用多因素方差分析法研究了光谱标准样品的均匀性．实验结果表明，除横向因素、纵向因素及二者的交互作用外，应将时间效应作为一个独立的因素，从而揭示出标样中较轻微的不均匀性．     

Recent progresses of polymer crystallization studied by AFM

The crystallization of polymers has been one of the major topics in polymer physics. Spherulites are common crystalline structures observed in semi-crystalline polymers. Many studies[1-5] have been performed to investigate the detailed structures and formation mechanisms. It is generally accepted that the three-dimensional geometry of spherulites develops from a stack of lamellae.     

平衡方法及平衡和解冻温度对牛体外受精胚胎玻璃化冷冻保存效果的研究

以体外成熟、体外受精并体外发育的牛囊胚为实验材料进行玻璃化冷冻保存，分别探讨了防冻剂的平衡方法以及平衡和解冻温度对胚胎冷冻效果的影响．实验结果显示，在室温下（１８℃）以二步法或三步法添加防冻剂的冷冻效果，明显优于在４℃下，以二步法添加防冻剂的效果，其冷冻—解冻胚的发育率分别为６２．８％（５９／９４）、５３．１％（５２／９８）和１４．６％（１２／８２）（Ｐ＜０．０１）；另外，室温下以二步法玻璃化冷冻的胚胎，在２０℃水浴中解冻的发育率明显高于３７℃解冻的效果，６２．７％（３７／６２）、２６．２％（１６／６１）（Ｐ＜０．０１）．结果表明：体外成熟、体外受精的牛胚胎可以进行玻璃化冷冻保存，并能得到较高的培养存活率，在室温下，以二步法添加防冻剂，并于２０℃下解冻的玻璃化冷冻保存方法是一种快速、简便而有效的方法．     

Activation of human CD4+ cells with CD3 and CD46 induces a T-regulatory cell 1 phenotype

The immune system must distinguish not only between self and non-self, but also between innocuous and pathological foreign antigens to prevent unnecessary or self-destructive immune responses. Unresponsiveness to harmless antigens is established through central and peripheral processes. Whereas clonal deletion and anergy are mechanisms of peripheral tolerance, active suppression by T-regulatory 1 (Tr1) cells has emerged as an essential factor in the control of autoreactive cells. Tr1 cells are CD4+ T lymphocytes that are defined by their production of interleukin 10 (IL-10) and suppression of T-helper cells; however, the physiological conditions underlying Tr1 differentiation are unknown. Here we show that co-engagement of CD3 and the complement regulator CD46 in the presence of IL-2 induces a Tr1-specific cytokine phenotype in human CD4+ T cells. These CD3/CD46-stimulated IL-10-producing CD4+ cells proliferate strongly, suppress activation of bystander T cells and acquire a memory phenotype. Our findings identify an endogenous receptor-mediated event that drives Tr1 differentiation and suggest that the complement system has a previously unappreciated role in T-cell-mediated immunity and tolerance.     

Identification of Stk6/STK15 as a candidate low-penetrance tumor-susceptibility gene in mouse and human

Linkage analysis and haplotype mapping in interspecific mouse crosses (Mus musculus x Mus spretus) identified the gene encoding Aurora2 (Stk6 in mouse and STK15 in human) as a candidate skin tumor susceptibility gene. The Stk6 allele inherited from the susceptible M. musculus parent was overexpressed in normal cells and preferentially amplified in tumor cells from F(1) hybrid mice. We identified a common genetic variant in STK15 (resulting in the amino acid substitution F31I) that is preferentially amplified and associated with the degree of aneuploidy in human colon tumors. The Ile31 variant transforms rat1 cells more potently than the more common Phe31 variant. The E2 ubiquitin-conjugating enzyme UBE2N was a preferential binding partner of the 'weak' STK15 Phe31 variant form in yeast two-hybrid screens and in human cells. This interaction results in colocalization of UBE2N with STK15 at the centrosomes during mitosis. These results are consistent with an important role for the Ile31 variant of STK15 in human cancer susceptibility.     

5 种两栖爬行动物首次在广东省发现

记述了广东省两栖动物新记录2种：大花角蟾Megophrys giganticus、黑眼睑小树蛙Phylautus gracilipes以及爬行动物新记录3种：半叶趾虎独山亚种Hemiphylodactylus yunnanensis dushangnensis、广西后棱蛇Oligodon guangxinsis、侧条后棱蛇Oligodon Lateralis、半叶践虎属Hemiphylodactylus亦为广东省新记录．